Welcome National Reference Centre (NRC) Mieke Reyniers National - - PowerPoint PPT Presentation

Welcome National Reference Centre (NRC)

Mieke Reyniers

National Plant Protection Organization (NPPO-NL) The Netherlands

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NRC: part of the plant health system in the Netherlands

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Importance of good infrastructure

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• Naktuinbouw: the Netherlands Inspection

Service for Horticulture

• BKD: the Flower Bulb Inspection Service
• NAK: the Dutch General Inspection Service
• KCB: the Quality Control Bureau

Plant health system in the Netherlands: government

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National Reference Centre (NRC)

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2500 fellow workers Headquartered in Utrecht Inspection offices throughout the country Wageningen: regional office + laboratories Independent agency in the Ministry of Agriculture, Nature and Food Quality Delivery agency for the Ministry of Health, Welfare and Sport.

Dutch-NPPO/NRC part of the Netherlands Food and Consumer Product Safety Authority (NVWA)

Meat supply chain and Food safety Veterinary medicinal products Export Import Product safety Animal welfare Hospitality industry and artisanal production Alcohol and tobacco Plant Health Nature Fish, fish products and aquaculture Animal testing Crop protection Animal Health Fertilizer Animal feed European subsidies Cross compliance Ground-bound subsidies Industrial production Animal by-products Microbioloy

NVWA covers 23 domains

Head Mieke Reyniers Plant Diseases Maikel Aveskamp

• Bacteriology
• Mycology
• Virology

Plant Pests Anton van der Sommen

• Entomology
• Nematology
• Invasive plants
• Centre for Monitoring vectors

Diagnostic methods Erik Souer

• Administration
• Biosafety/-security
• Molecular Biology
• Greenhouses

National Reference Center – plant health

more than a lab

Organization chart NRC

Head Mieke Reyniers Plant Diseases Maikel Aveskamp

• Bacteriology
• Mycology
• Virology

Plant Pests Anton van der Sommen

• Entomology
• Nematology
• Invasive plants
• Centre for Monitoring vectors

Diagnostic methods Erik Souer

• Administration
• Biosafety/-security
• Molecular Biology
• Greenhouses

National Reference Center – plant health

more than a lab

Tasks of the NRC

Knowledge

NRC has an independent, scientific based position within the NVWA

Contribution to science: diagnostic

methods, diagnostic protocols, taxonomy, biology and epidemiology

Supervision/contribution to research projects: eg H2020 (EU), EUPHRESCO (EPPO),

PPS (Min. EZK)

Knowledge Working on pest risk analysis, quick scans (EU, EPPO, EFSA) Participation in International meetings Maintenance of network (lab visits)

Methods Method development: 1) Test Development

literature, third parties, own research

2) (Lab-)Validation

Robust, specific, reliable

3) Implementation

Diagnosis

• Clients:
• NVWA inspection
• Agricultural Inspection Agencies

(reference samples)

• Third parties

Mainly focus on quarantine-

• rganisms

(risk-based)

Diagnosis

• Microscopy

(Conventional, IF, EM)

• Biochemical tests

(eg DAS-ELISA, MALDI-TOF)

• In planta

(e.g. Koch’s Postulates, fysiodetermination)

• Molecular methods

(eg RealTime PCR, Sangersequencing new: High Throughput Sequencing)

Identification = Combination of at least two independent tests

Facilities Quarantine building Quarantine greenhouses Outdoor fields (open and contained) Diagnosis

Collections

• Collection ~ 230k specimens of

plant pests and pathogens (Reference material)

• Herbarium/ pinned insects /

microscopic slides

• Living collection
• On culture
• Symptoms
• Tissue and DNA

Education

• Training (inspectors of NVWA, inspectors
• f Agricultural Inspection Agencies, EU, public

networks, EUPHRESCO)

• Capacity building and Twinning

(eg Turkey, Vietnam, Egypt, Kenya)

• Internships

(applied-) universities, college

Advice NVWA, Ministry

eg Risk assessments, Legislation 3rd countries (export), elimination scenarios, …

Inspection

Sampling methods, risk-based inspection,…

EPPO, EU, IPPC

Diagnostic protocols, Methodologies, …

Supervision Labs of the Agricultural Inspection Agencies (NAK, Naktuinbouw, BKD, KCB)

• Training
• Advice
• Auditor: ISO 17025 Quality Management

implementation

All labs working with Q-organisms

• Auditor: 2008/61 Biosecurity implementation

Potential threats: Signaling (literature/finding/third country) Quick-scan Pest Risk Analyses (PRA) Risk Assessment

Quality Management System

• ISO17025 accredited
• Fixed scope (since 2014)

Accreditation is limited to predefined host- matrix combinations

• Flexscope (since 2017)

Extension of accreditation is possible for all host-matrix combinations

www.rva.nl

Biosafety & Biosecurity

• Internal

Make sure that organisms stay inside

• External

Advise on working with Q-organisms under 2008/61

• labs of the Agricultural Inspection Agencies
• other phytosanitairy labs,
• breeding companies,
• research institutes,
• universities

Goals workshop:

• expanding professional network
• sharing knowledge via presentations and posters
• identify shared research needs