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Volatile Metabolites as Early Diagnostics for Blood-Borne Pathogens
- Dr. Yatin J. Mange
Syft Technologies Ltd September 2, 2016 yatin.mange@syft.com
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Volatile Metabolites as Early Diagnostics for Blood-Borne Pathogens - - PowerPoint PPT Presentation
Volatile Metabolites as Early Diagnostics for Blood-Borne Pathogens - - PowerPoint PPT Presentation
Volatile Metabolites as Early Diagnostics for Blood-Borne Pathogens Dr. Yatin J. Mange Syft Technologies Ltd September 2, 2016 yatin.mange@syft.com www.syft.com Summary Introduction to SIFT-MS SIFT-MS for the detection of blood-borne
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Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) – Key Attributes
Analytical technique for measuring Volatile Organic Compounds (VOCs) Rapid and real-time measurement Selectivity, sensitivity and speed for a diverse range of chemical species Soft ionization resulting in less fragmentation Detection limit up to pptv Easy to operate
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SIFT-MS – how this soft chemical ionization technique works
Multiple reagent ions Pure reagent ion delivery Ultra-soft sample ionization Mass spectrometer
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SIFT-MS can measure most VOCs and inorganic compounds hydrocarbons
alkanes, alkenes, aromatics, monoterpenes
- xygenates
alcohols, aldehydes, ketones, esters, ethers, carboxylic acids, formaldehyde
nitrogen compounds
amines, amides, nitriles, nitrated organics
sulfur compounds
mercaptans, thioethers, carbonyl sulfide
halogenated compounds
aliphatic and aromatic fluorides, chlorides, bromides and iodides
inorganics
ammonia, hydrogen cyanide, hydrogen sulfide, hydrogen chloride, hydrogen fluoride, carbon dioxide, sulfur dioxide,
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Who is using Syft Instruments
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SIFT-MS is extremely sensitive and has a dynamic range of 6 orders of magnitude
0.01 0.1 1 10 100 1000 10000 100000 10 20 30 40 50 60 70 80 90 100 110 120
Concentration (ppb) Time (Seconds)
DYNAMIC RANGE 1,3-butadiene: 30 ppt benzene: 15 ppb benzene: 30 ppm pptv sensitivity 1,000-fold increase in benzene has no effect
- n 1,3-butadiene
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Concentrations can be monitored in real time
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SIFT-MS for the detection of blood-borne pathogens
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SIFT-MS - Biomedical applications
Breath diagnosis – non-invasive, point-of-care diagnostics and monitoring:
- Diagnosis of Chronic Obstructive Pulmonary Disease (COPD) and asthma.
- Determination of dialysis efficacy
- Diagnosis of acute renal failure and transplant rejection
Laboratory – high throughput, low operator skill:
- Detection and identification of bacteremia in blood culture
- Diagnosis of urinary tract infections
- Detection of solvents and toxic industrial chemicals in urine
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SIFT-MS application – VOCs from Blood-Borne Pathogens
VOCs present as a result of metabolic or disease processes Present in breath, or headspace over body fluids or cultures Conventional automated systems detect CO2 liberated by growing microbes. (Eg: BacT/ALERT techniques) Conventional techniques – Time consuming, trained technician Timely detection enables more accurate treatment In the current study, 5 bacterial species detected from 10 CFUs
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Identification of bacterial pathogens from blood cultures
Bacterial VOCs at 6 hours in BacT/ALERT medium
SIFT-MS decreased time-to-positive result by as much as
- ne half
Enhanced sensitivity enables detection of bacterial growth in blood culture in 6 hrs VOCs from each bacterial culture headspace completed in 30 s without prior sample preparations Profiles of MVOCs allows differentiation between different species of bacteria Potential for integration into automated systems Bacterial Species
Pseudomonsa aeruginosa Streptococcus pneumoniae Escherichia coli Staphylococcus aureus Neisseria meningitidis
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Automated SIFT-MS analysis – VOCs from headspace
Existing BacT/ALERT culture system Selected Headspace “Real-time” results
Automated Syft Voice200ultra analysis workstation
Bacteria 1 Bacteria 2 Bacteria 3 Bacteria 4
12hrs 24hrs 36hrs 48hrs 60hrs 72hrs SIFT-MS enhanced standard
identification
Bacteria 1 Bacteria 2 Bacteria 3 Bacteria 4
Antibiotic response
No change to existing procedure!
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Summary
SIFT-MS technology is adaptable, testing a range of body samples (including breath, blood and urine) Volatile compounds produced by microbes are measured quickly and accurately Unique set of volatile metabolites indicates particular species of bacteria Shorter incubation time and rapid analysis – Powerful early screening tool
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Find out more at Exhibition Hall 1 (1F) Poster No: PA-94
Questions?
Email: yatin.mange@syft.com