Vermicomposting agricultural waste into value- p g g added - - PowerPoint PPT Presentation

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Vermicomposting agricultural waste into value- p g g added - - PowerPoint PPT Presentation

Cyprus conference, 2016 Vermicomposting agricultural waste into value- p g g added products: 30yr s R & D in China Zh Zhenjun SUN j SUN China Agricultural University Beijing Vermitech Institute Email sun108@cau.edu.cn @


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Cyprus conference, 2016

Vermicomposting agricultural waste into value- p g g added products: 30yr’s R & D in China Zh j SUN Zhenjun SUN China Agricultural University Beijing Vermitech Institute Email:sun108@cau.edu.cn

College of Resources and Environmental Sciences, CAU

@

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Cyprus conference, 2016

The Status of Agricultural Waste Utilization in China Total agricultural waste resources:1 billion tons, including g , g Crop straws: 0.7 billion tons; livestock manures: 0.18 billion tons

Manure fertilizer 10% Directly returning to field 10% returning to field 50% Bi Directly returning to field Burning 40% Biogas production 3% returning to field 20%

straws manures

eloped rea

Industrial Organic fertilizer 5%

manures

U t t d 40

Deve ar

materials 3% Surplus 30-40%

Surplus 70

Environmental Untreated 40- 45%

College of Resources and Environmental Sciences, CAU

Surplus 70- 80%

Environmental pollution

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Cyprus conference, 2016 A i lt l Ci l E D l t P tt Coordination Agricultural Circular Economy Development Pattern

Agr

Small Conventional Live Resource Coordination

ricultu

Internal circulation Circular agriculture Ecological

Production Environment

ural w

L t l Circular agriculture g agriculture Agriculture and ecology Life Society Economy

waste

Large external circulation Agriculture and industry combination Life Economy

Circular society

College of Resources and Environmental Sciences, CAU

Circular society

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Cyprus conference, 2016 Use strategy Methods Problems Results Methods Problems Results

Large Quantity

Efficient Quantity

Poor Quality

High quality Quality

Quality Low value

Industrial

Value

Harmful

Recycle Harm College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

Ecological Utilization

Straw returning Methane production Methane production Edible fungi g Vermicomposting Agriculture and livestock composting Multiple integration

College of Resources and Environmental Sciences, CAU

Multiple integration

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Hi h Q lit U High Quality Use

Commercialization and Marketization New products: Bi l i l i (Bi l i l f ili Biological preparation(Biological fertilizer、 Biological pesticides Etc ) Biological pesticides Etc.) Biochemical preparation(Amino acids Biochemical preparation(Amino acids、 Enzyme、Oligosaccharides Etc.)

College of Resources and Environmental Sciences, CAU

Enzyme、Oligosaccharides Etc.)

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产品 15000 16000 产品 12000 14000 6000 8000 10000 750 4000 6000 150 300 750 2000 原料 传统堆肥 商品有机肥 叶面肥或酶制剂 木寡糖或抗菌肽 原料 传统堆肥 商品有机肥 叶面肥或酶制剂 木寡糖或抗菌肽

Comparison of the added value of the products developed Comparison of the added value of the products developed from agricultural waste from agricultural waste

College of Resources and Environmental Sciences, CAU

Comparison of the added value of the products developed Comparison of the added value of the products developed from agricultural waste from agricultural waste

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Cyprus conference, 2016

Vermiculture in China

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

f ff i i l i ld factors affecting vermiculture yield

thi k materials thickness density residuals→ earthworm→

Vermiculture

earthworm→ vermicast→ harvest conditions Bed design

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

Simple windrow system p y

大田养殖 大田养殖

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

Plastic Greenhouse System in North China

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

Composting pretreatment

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

Intercroping system: p g y vermiculture in field

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

Intercropping of vermicomposting and banana

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

A ib i l id i h Ei i F id Antibacterial peptides in earthworm Eisenia Fetida

Sun (1995) found an antibacterial peptide that was composed of f i id f h fi i h ld forty amino acids from earthworm at first in the world.

Name Sequence of amino acid Number of amino acid Molecular weight

Antibacterial characteristic

acid amino acid weight

characteristic

EP1 / 40 4832 Bacteria and fungi resistance EP2 Ac-Ala-Met-Val-Ser-Ser 6 535.27 Bacteria resistance EP3 Ac-Ala-Met-Val-Gly-Thr 6 519.27 Bacteria resistance y EP4 / / About 20000 Bacteria resistance ECP5-1 Ala-Cys-Ser-Ala-Gly 5 510.80 Bacteria resistance

College of Resources and Environmental Sciences, CAU The methods of earthworm peptide separation and purification have achieved the patent in 2004.

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Sequence of amino acid in ECP5-1

College of Resources and Environmental Sciences, CAU

Ala-Cys-Ser-Ala-Gly

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Cyprus conference, 2016 Influence of peptide on cancer cells

Th i f EP d EP MGC803 ll The resistance of EP2 and EP3 to MGC803 cell

Scanning electron microsoopical observation on MGC803 Scanning electron microsoopical observation on Scanning electron microsoopical observation on MGC803 cells which was untreated with antibacterial peptides Scanning electron microsoopical observation on MGC803 cells treated with EP2 for 24h Scanning electron microsoopical observation S i l t i i l b ti

College of Resources and Environmental Sciences, CAU

Scanning electron microsoopical observation

  • n MGC803 cells treated with EP2 for 48h

Scanning electron microsoopical observation

  • n MGC803 cells treated with EP3 for 24h
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The character of earthworm antibacterial peptides

  • The antibacterial peptide had stable physicochemical

properties such as heat stability and wide pH scope properties such as heat stability and wide pH scope. There was little influence of pH 3, 5, 7, 9, 11 on the antibacterial activities of antibacterial tetradecapeptide antibacterial activities of antibacterial tetradecapeptide. And the antibacterial tetradecapeptide could keep when storing 180 days in home temperature storing 180 days in home temperature.

  • The antibacterial mechanism and character of the five

earthworm peptides was also researched in our group .

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 Influence of Escherichia coli on the antibacterial function from Eisenia fetida We have a new finding that fat from earthworm have the We have a new finding that fat from earthworm have the function of bacteria resistance. College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 Vermicasts as function fertilizer College of Resources and Environmental Sciences, CAU The resistance of vermicompost to anthracic of cucumber

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Cyprus conference, 2016 Two function microbes from vermicompost 0103A (Streptomyces globisporus) 0104A (Streptomyces syringini) ( y y g )

Fibrillae of 0103A spores Spores of 0103A Spores of 0104A Fibrillae of 0104A spores

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

Bioreactor for waste conversion

College of Resources and Environmental Sciences, CAU

Bioreactor for waste conversion

A Chinese patent

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Function liquid fertilizer Earthworm Products

College of Resources and Environmental Sciences, CAU

Earthworm Products

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Earthworm Products

College of Resources and Environmental Sciences, CAU

Earthworm Products

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Cyprus conference, 2016

Some products developed in CAU

① products processed from earthworm

  • d f

l

  • amino acid for animal nutrition
  • foliar fertilizer with amino acid
  • amino acid biopestcide

② e i o

  • t

② vermicompost:

  • commercial fertilizers
  • soil less culture medium

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

amino acid processing from earthworm

Worm →hydrolyzing with enzyme

→ raw amino acid → adding microelement → raw amino acid → adding microelement →AA additives → chelating → chelated AA →AA foliar fertilizer

g

→AA pesticides

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

amino acid products: biochemical fertilizer

College of Resources and Environmental Sciences, CAU

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Micro‐ecological adjustant and function fertilizer function fertilizer

① Some effective antagomistic microorganisms g g were selected from vermicompost (Streptomyces spp) ( p y pp) ② Micro‐ecological adjust agent and soil conditioner plant rhizogenic agents conditioner plant rhizogenic agents ③ Antibacterial fertilizers and soil regeneration ( Soil‐borne disease of Cucumber seedlings ) ( Soil‐borne disease of Cucumber seedlings ) ④ Multi function fertilizers

College of Resources and Environmental Sciences, CAU

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Bacterium(0103A) from vermicast

College of Resources and Environmental Sciences, CAU mycelium

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Cyprus conference, 2016

Bacterium(0103A) from vermicast

College of Resources and Environmental Sciences, CAU

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E h id d Earthworm peptide and new pesticide‐fertilizer new pesticide‐fertilizer

① 4 peptides extracted from earthworms ① 4 peptides extracted from earthworms ② effective methods for earthworm peptide

processing (3 patents)

③ 3 producing lines in China for the new ③ 3 producing lines in China for the new

fertilizer

④ 2 nation certificates for new organic

fertilizer

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 pesticide-fertilizer made of earthworm in China College of Resources and Environmental Sciences, CAU pesticide-fertilizer made of earthworm in China

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New products and Application of anti New products and Application of anti‐ bacterium fertilizers in China bacterium fertilizers in China bacterium fertilizers in China bacterium fertilizers in China

1 Multi 1 Multi function biological organic fertilizer: function biological organic fertilizer:

  • 1. Multi
  • 1. Multi‐function biological organic fertilizer:

function biological organic fertilizer: Vermicomost and effective microorganism Vermicomost and effective microorganism 2 AA f li f ili 2 AA f li f ili

  • 2. AA foliar fertilizer:
  • 2. AA foliar fertilizer:

Earthworm and microelements Earthworm and microelements 3 A ib i l id f ili 3 A ib i l id f ili

  • 3. Antibacterial peptide fertilizer:
  • 3. Antibacterial peptide fertilizer:

Biochemistry, earthworm Biochemistry, earthworm 4 V i 4 V i 4.Vermicompost tea: 4.Vermicompost tea: Vermocompost and effective microorganism Vermocompost and effective microorganism

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

Antiblastic effect of vermicompost on some plant disease

发病率 (%)

p

d cd a b cd d c a 70 80 鲜重 (0.1g) d d b b b a a 40 50 60 70 株高(0 1 ) 株高 (0.1cm) d cd c c b c c b b c c 10 20 30 发病率(%) 鲜重(0.1g) 株高(0.1cm)

CK 10%V 20%V 30%V 50%V 20%V0 健康ck 健康20%V

处理

College of Resources and Environmental Sciences, CAU (V-Vermicompost)

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Cyprus conference, 2016

prevention and cure effect on cucumber

College of Resources and Environmental Sciences, CAU

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Application on vegetable production

College of Resources and Environmental Sciences, CAU

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On flowers and

  • rnamentals

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 Experiment of Earthworm AA additive on fish College of Resources and Environmental Sciences, CAU p (Qingdao, Shandong, China)

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Cyprus conference, 2016 Experiment of Earthworm AA additive on pig College of Resources and Environmental Sciences, CAU

CK AA additive

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Cyprus conference, 2016 Experiment of vermicompost as culture medium on vegetables (Shunyi, Beijing) College of Resources and Environmental Sciences, CAU

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Application of vermicompost on alone (T h B iji ) (Tongzhou, Beijing)

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

Wide foreground of vermiculture and vermiproducts in organic food production g p

College of Resources and Environmental Sciences, CAU

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Earthworm (cast) and products processing

Bio-organic fertilizer vermicasts Vermicompost tea D d i i Deodorization i ti l Organic waste vermicomposting earthworms vermiceuticals Vermimeal earthworms Vermimeal Amino acids College of Resources and Environmental Sciences, CAU

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earthworm industrial chain

biomedicine biomedicine

feedstuff

Health

Earthworm

fertilizer foods cosmetics College of Resources and Environmental Sciences, CAU

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Medicine enzymes and Cosmetic products

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016

Medicine enzymes from earthworms

College of Resources and Environmental Sciences, CAU

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Cyprus conference, 2016 College of Resources and Environmental Sciences, CAU Cosmetic products made from earthworm

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