Tissue Engineering and Regenerative Medicine Alonzo D. Cook, PhD - - PowerPoint PPT Presentation

Tissue Engineering and Regenerative Medicine

Alonzo D. Cook, PhD Chemical Engineering Dept. cook@byu.edu (801) 422-1611

Areas of Interest: Biomedical Engineering; Cardiovascular repair; Stem Cells; Neuroscience; Vision; Renal Function; Orthopedics

• Heart
• Kidney
• Nerve
• Blood Vessel
• Eye
• Pancreas

Cook Lab Projects

Fresh pig heart Decellularized pig heart

Decellularized Pig Heart

Heart Project

• Remove cells from pig hearts

(decellularization)

• Culture human cells

(stem cells, cardiomyocytes)

• Visualize cells in 3D inside heart tissue
• Test recellularized hearts for function

(beating, pumping)

• Prevent thrombosis, hemolysis of blood

IPS Cells Beating on Porcine Matrix

Kidney Project

• Remove cells from pig kidneys
• Culture human cells

(stem cells, epithelial cells, endothelial cells, etc.)

• Visualize cells in 3D inside kidney tissue
• Measure DNA changes after recellularization
• Test recellularized kidneys for function
• Prevent thrombosis, hemolysis of blood

Fresh pig kidney Decellularized pig kidney Fresh kidney Decellularized kidney

Nerve Project

• In situ decellularization of sciatic nerve in rats
• Crush injury of nerve
• Chemical injury of nerve
• Combination of crush and chemical injury
• Measurement of loss of action potential
• Addition of nerve growth factor (NGF)
• Analysis of rate of nerve regeneration
• Application to Diabetic Rat model

Blood Vessel Project

• Printing cells in alginate gels
• Culturing 3D blood vessels
• Crosslinking gels in the presence of cells
• Modifying gels for covalent crosslinking

Cells encapsulated in alginate hydrogel

Nuclear power, nuclear safety, and nuclear reactor design

Matthew Memmott

Chemical Engineering memmott@byu.edu

801-422-6237

Areas of Interest:

Enhancing the passive safety of both current and advanced nuclear reactor technology while improving the economics, fuel utilization, and grid adaptability of current plants

• Large, inter-institutional efforts of reactor

concept designs:

• Advanced LWR (I2S-LWR) – solve Fukushima problems
• Molten Salt Reator – In situ waste removal, liquid fuel
• Sodium Fast Reactor – waste reprocessing, safety
• System Design
• Materials
• Structural Analysis & CAD Modeling
• Chemical Separations
• Design Transients
• Safety Analysis

New Nuclear Reactor Concepts

• utlet

Enhanced Safety

• Development of Passive Safety Systems
• New Components

– Compact Heat Exchangers – Core Shutdown Devices (in addition to control rods)

• New Concepts

– Passive Endothermic Reaction Cooling System (PERCS) – Leidenfrost Pumps (passive water pumps) – Others

Energy Integration Technology

• Thermal Storage

– Supercritical Fluids – Grid Integration – Transient Optimization – Nuclear Integration/Safety

• Thermodynamics

– Modeling – Experiments

• System Design & Process Controls
• Structures & Materials
• Dynamic Optimization

Accident Tolerant Fuel Concepts

• Nuclear fuel that:

– Tolerates higher temperatures – Limits Fission Gas Release – No fuel & clad/coolant interactions – Structurally Stable (vibration) – Easily Fabricated

• CFD
• Heat Transfer
• Neutronic Analysis
• Material/Chemistry/

thermodynamics

Bill Pitt

Chemical Engineering

801-422-2589; pitt@byu.edu

Research area: Drug Delivery I can deliver stuff to the cell cytosol. I am looking for collaborative applications of this technology.

We can deliver drugs to cell cytosol

No emulsions in the liposomes Emulsions in the liposomes

• Delivery of the fluorescent molecule calcein using folated

eLiposomes and 20 kHz ultrasound at 1 W/cm2 for 2 seconds.

Javadi et al., J. Controlled Release 2013

Folate, US, and emulsions are required for internal delivery.

We can deliver plasmids

Confocal image of HeLa cells exposed for 2 hours to ultra eLiposomes containing plasmid, followed by application of 20-kHz ultrasound at 1W/cm2 for 2 seconds. (A) eLiposomes were not folated. (B) eLiposomes contained folate in their phospholipid membrane. (C) Folate receptors were already blocked with extra folate before adding the eLiposomes. Pictures were taken 48 hr after applying the ultrasound.

• A. Non-folated. B. Folated eLiposomes C. Competitive binding by folate

Overcoming Multi-drug Resistance of Cells

We are looking for collaborators who have resistant and sensitive cell lines

Overcoming Multi-drug Resistance of Cells

We are also looking for collaborators who have antibodies to proteins expressed uniquely on cancer cell surfaces.

Contact: Bill Pitt pitt@byu.edu

MRI Imaging of Metastatic Cancer Sites

• Developing strategies to bind MRI contrast

agents to tumor cells and neovascularization

• Prelim results in phantom tissue shows that

we can detect my markers in volumes as small as 1 uL, and at concentrations as low as 20 µM

• Looking for collaborators with unique

ligands targeting cancer tissue

CELL-FREE SYNTHETIC BIOLOGY

BRADLEY C. BUNDY

Associate Professor

• Dept. of Chemical Engineering

Brigham Young University bundy@byu.edu http://bundy.byu.edu

Our Research Motivation = Life

Cell-free Synthetic Biology

RNA Polym- erase

Amino Acids New Peptide

• E. coli

DNA

Messenger RNA

Ribo- some Ribo- some

Energy Source In vitro (cell-free)

Smith MT, Wilding KM, Hunt JM, Bennett AM, Bundy BC. 2014. The Emerging Impact of Cell-free Synthetic Biology. FEBS Letters. 588(15):2755-2761.

Direct Access = Optimization, Transport, No toxicity

Heterologous Machinery

Cell-free Research Projects

Excited about creating or joining research teams!

DNA mRNA RNApol Ribosome Nascent Polypeptide VLP: Vaccines, Imaging, Catalysts Biocatalysts: Immobilization, PEGylation Genetic Recoding: w/ Unnatural Amino Acids Biosensors “Just Add Water” Therapeutics

Interdisciplinary Communication: Precision vs Understanding

Joseph Ekstrom Information Technology School of Technology jekstrom@byu.edu (801) 422-1839 Areas of Interest:

Terminology management, network and systems management, distributed computing, system modeling and architecture, system development, information assurance, penetration testing and IT curriculum and development

Plato’s Academy

The Western University

Are they inevitable?

Communities of Interest

• Form around a common interest
• Evolve a vocabulary to communicate about that interest
• Codify that common vocabulary, often in a glossary
• Reuse terms that are “close”
• Invent multi-word terms which become acronyms
• Specialize meanings (Consider the word process in your discipline)
• Create a new silo

Can Technology Help?

• Libraries no longer have walls.
• Information is a click away.
• Are there ways to facilitate sharing terminology?
• Are there tools to help us identify conflicting terminology?
• Is it possible to avoid “violent agreement” in interdisciplinary

meetings?

Termediator.byu.edu

• Prototype for “terminological mediation”
• 500 Glossaries
• 18 Domains (communities of interest)
• 50000 Terms
• 80000 Definitions
• Mean 1.7 terms/definition
• Some more than 100 terms/definition
• Most common are universal terms
• Some are very polysemous (many meanings)
• How many are synonymous?

Interested?

• Point us to a glossary
• Help us put glossaries into normalized form (XML)
• Help us improve the quality of the corpus
• Help us imagine tools
• Help us implement tools
• Help us test tools

Analog/Mixed Signal Integrated Circuits

Shiuh-hua Wood Chiang Assistant Professor Department of Electrical and Computer Engineering 801-422-6749 wochiang@byu.edu

Speed Network 2015

Does Your Lab Look Like This?

36

Does Your Lab Look Like This?

37

Advantages of going integrated:

• Smaller size
• Less power
• Higher precision and speed

Ultra Low-Noise Ion Detector

38

• Gain: 80 dB
• Input-referred noise voltage: 17 uVrms
• Detects <100 electron charge

Collaborators: Dr. Hawkins, Dr. Milton Lee, PerkinElmer

Ultra Low-Power SAR ADC

39

Yau ISSCC 2014 Liu VLSI 2010 Chang VLSI 2011 Hershberg VLSI 2013 This work

Tech (um)

0.18 0.18 0.18 0.18 0.18

Speed (MHz)

0.004 10 0.031 20 10

SNDR (dB)

59.3 60.3 45.1 75.9 53.4

Power (uW)

0.031 98 0.087 2960 27.5

FoM

(fJ/conv- step) 10.3 11.6 18.9 29.0 7.19

3d printed microfluidics

Gregory P. Nordin Electrical and Computer Engineering nordin@byu.edu (801) 422-1863

Areas of Interest:

3D printed microfluidics, biological and chemical sensors, nanophotonics and integrated optics, micro- and nanofabrication, MEMS, and microfluidics

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python
• 90 nm trench in

1.6 mm wide silicon rib waveguides

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python

60 nm nanochannel array

• n 15 mm wide quartz pedestal

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python

3 mm Diameter Valve 1.5 mm Diameter Valve

Rogers et al. Biomicrofluidics 2015, 9, 016501.

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python

Research Areas

• Device micro- and nanofabrication
• Cleanroom in Clyde Building
• E-beam lithography
• Focused ion beam
• 3D printing
• Microfluidics
• PDMS
• PEGDA (collaboration with Adam Woolley in Chemistry)
• Integrated valves and pumps
• Integrate with silicon, quartz, and glass sensing substrates
• 3D printing for microfluidics
• Sensor technologies
• Nanochannels
• Impedance
• Fluorescence
• Microcantilevers
• Surface functionalization

Other:

• Optics & photonics
• EM & fluid dynamics simulation
• Microcontrollers, sensors, & actuators
• Develop our own instrumentation
• Replace Matlab/LabView with Python

BYU MRI Research Facility

Neal Bangerter Electrical and Computer Engineering neal_bangerter@byu.edu 801.422.4869

Erin Bigler Emeritus Director Professor, Psychology Jonathan Wisco Associate Director Associate Professor, P.D. Biology Neal Bangerter Director Associate Professor, Electrical Engineering Brock Kirwan Associate Director Assistant Professor, Psychology

MRI Research Facility

• Whole body 3 Tesla MRI scanner (Siemens)
• Broad array of coils for imaging
• Extremities
• Knees, hips, wrists, shoulders
• Spine and neck
• Abdomen, pelvis, and heart
• Head
• Mouse coil also available
• Multi-nuclear capabilities
• Can image 1H, 23Na, and other nuclei
• Bangerter group can help with custom pulse sequences, custom coils

for targeted applications

MRI Research Facility - Seed Grant Program

• $10K seed grants
• Over $300K invested to date
• Goal: Enable acquisition of pilot data for extramural

proposals

• Next round: March 2013

Groups with MRI Research Expertise

• Neal Bangerter (Electrical & Computer Engineering, Neuroscience)
• MR physics, MR pulse sequence development
• Protocol development and contrast optimization
• Multi-nuclear MRI (sodium)
• Quantitative MRI techniques
• Focus on musculoskeletal and cardiac applications
• Brock Kirwan (Psychology, Neuroscience)
• fMRI experiment design
• fMRI data analysis
• Focus on memory
• Jonathan Wisco (Physiology and Development Biology, Neuroscience)
• Anatomical imaging
• Small animal imaging
• Study of Alzheimer’s Disease
• Erin Bigler (Psychology, Neuroscience)
• Neuroimaging, including diffusion tensor imaging (DTI)
• Traumatic brain injury
• Mikle South (Psychology, Neuroscience)
• fMRI experiment design
• fMRI data analysis
• Focus on autism
• And others…
• Michael Larson (Psychology, Neuroscience)
• Shawn Gale (Psychology, Neuroscience)
• Business school, Computer Science, Political Science, Exercise Science, Communications, Civil Engineering, Mechanical

Engineering

FLOW Lab: FLight, Optimization and Wind Lab

Andrew Ning Mechanical Engineering aning@byu.edu (801) 422-1815 flow.byu.edu Areas of Interest: Multidisciplinary Optimization; Wind Energy; Aircraft Design; Aerodynamics; Aeroelasticity; Uncertainty Quantification; Computational Methods

Wind Energy

Vertical Axis Wind Turbine Wakes

quietrevolution, qr5

Wind Farm Optimization

Wind Farm Acoustics

Aero-Structural Blade Design

Aeronautics

Passive Flow Control of Small Propellers

Unmanned Aerial Vehicle Trajectory Optimization

Influence of Aspect Ratio on Heat and Mass Transfer

Brian Iverson Mechanical Engineering bdiverson@byu.edu 801-422-7514

Areas of Interest:

• Enhanced convective transport
• Microfabrication of sensors and actuators
• Absorptive surfaces for solar energy collection
• Power systems for renewable energy

Dynamic Radiative Surface Properties with Origami-Inspired Topography

Apparent Absorptivity Apparent Emissivity Net Radiative Heat Exchange 2015, Journal of Heat Transfer

Advection Enhanced Chemical Reactions

200 μm

10 µm

(a) (b) (c) (d) (e) (f)

2015, ACS Nano

Advection Enhanced Chemical Reactions

4 µm

200 nm

IA State, Naval Research Lab

50% w/w H2O2

Advection Enhanced Chemical Reactions

68

Potentiostat

114 μM

Complete utilization of analyte Critical for low concentration solutions

• Condensation at

superhydrophobic surfaces

• Thermal isolation using

nanoporous films

Collaboration:

• D. Maynes, J. Crockett

Collaboration: B.D. Jensen, R. Davis, R. Vanfleet

Surface Structures

Unifying Theme:

• Investigate control of surface composition/structure on transport