SLIDE 1
Thoughts on diploid inbred potato as a genetics resource
Shelley Jansky USDA-ARS and UW-Madison
Fernie and Klee, 2011
M6 (S. chacoense)
Self-pollinate
Recombinant Inbred Lines
Line 1 Line 2 Line 3 Line 4 Line 5 Line 100
Thoughts on diploid inbred potato as a genetics resource Shelley - - PowerPoint PPT Presentation
Thoughts on diploid inbred potato as a genetics resource Shelley - - PowerPoint PPT Presentation
Thoughts on diploid inbred potato as a genetics resource Shelley Jansky USDA-ARS and UW-Madison Recombinant Inbred Lines M6 ( S. chacoense ) Self-pollinate Line 1 Line 2 Line 3 Line 4 Line 5 Line 100 Fernie and Klee, 2011 Recombinant
SLIDE 2
SLIDE 3
Recombinant Inbred Lines
- Maintained as true seed
- Permanent mapping population
- Genotype each line once
- Phenotype for multiple traits
- Multiple recombination break points
- Improved mapping resolution
SLIDE 4
DM1-3 x M6 F2
SLIDE 5
DM1-3 x M6 F2 US-W4 x M6 F2
SLIDE 6
DM1-3 x M6 F2 C545 x M6 F2 US-W4 x M6 F2
SLIDE 7
DM1-3 x M6 F2 US-W4 x ber F2 C545 x M6 F2 US-W4 x M6 F2
SLIDE 8
SLIDE 9
SLIDE 10
F2 clone F4 family
SLIDE 11
F2 clone F4 family
SLIDE 12
20 40 60 80 100 F1 F2 F3 F4 F5
Percent heterozygous SNPs
DM1-3 x M6
F6
SLIDE 13
20 40 60 80 100 F1 F2 F3 F4 F5
Percent heterozygous SNPs
DM1-3 x M6
F6
SLIDE 14
SLIDE 15
“…the adaptive complexes of two distinct species when split up and segregated in the F1 cannot replace each other because the genetic architecture of the two species is too distinct. Thus after segregation many of the progeny will lack the full adaptive and developmental gene complexes of one or the other parent and genetic breakdown will
- ccur.” Hawkes (1990)
SLIDE 16
Self-pollination of hybrids
- F2 generation
- Hybrid vigor
- Hybrid breakdown
SLIDE 17
Self-pollination of hybrids
- F6 generation
- Inbreeding depression
- Hybrid breakdown
SLIDE 18
Introgression lines
Line 1 Line 2 Line 3 Line 4 Line 5
Backcross
SLIDE 19
Introgression Lines
- Each line carries a single DNA segment from the
donor parent
- All DNA of donor parent is represented in the set of
introgression lines
- Most DNA in each line is cultivated potato
- Improved fertility compared to RILs
- Identify QTL that were not detectable in RILs
SLIDE 20
US-W4 S5-15
SLIDE 21
Introgression lines
Line 1 Line 2 Line 3 Line 4 Line 5
US-W4 S5 M6
Line 50
Backcross
SLIDE 22