The OSCAR project: a collaborative network throughout Italy 406 V3 - - PowerPoint PPT Presentation

• 406 V3 sequences with HS

Trofile results available have been collected

The OSCAR project: a collaborative network throughout Italy

OSCAR has been the first study aimed at evaluating the performances of genotypic tropism testing using ESTA as reference in a so large cohort of patients

S5

(nt 684-703)

1 nt 1401 aa 1 467 792 298 894 264 AS1

(nt 1027-1047)

S6

(nt 682-702)

AS5

(nt 1292-1310)

S2

(nt 630-650)

AS3bis

(nt 1034-1051)

V3 V1 V2 V4 C1 C2 C3 C4 C5

The protocol for V3 sequencing set up in the framework

• f the OSCAR programme showed an overall efficiency
• f 84.2%

Location of the V3 loop amplification and sequencing primers on HIV-1 gp120 env coding

• region. Primers for PCR are shown in bold black. Primers used for sequencing reaction are

shown in grey

Overall Concordance between genotypic test and High Sensitivity Trofile

Geno2pheno at different FPR Concordance with HS Trofile N (%/365) G2P (5%) 296 (81.1%) G2P (10%) 286 (78.4 %)

Svicher et al., New Microbiologica, 2010

Identify new V3 genetic determinants correlated with different co-receptor usage, crucial to set up even more accurate genotypic prediction of HIV-1 tropism.  Evaluate the impact of FPR on HIV-related markers such as CD4-cell counts and HIV-RNA in ART naïve and experienced patients and on the time-to-achieve CD4 cell-count >350 cells/µl in drug-naïve patients starting a first line ART-regimen .  Define the correlation between FPR obtained by population V3- genotyping and the presence of HIV-1 variants with DM/X4-tropism, detected by both Enhanced-Sensitivity Trofile phenotypic assay (ESTA) and ultra-deep V3-pyrosequencing (UDPS).

The OSCAR project helped to:

The OSCAR project helped to:

Identify new V3 genetic determinants correlated with different co-receptor usage, crucial to set up even more accurate genotypic prediction of HIV-1 tropism.  Evaluate the impact of FPR on HIV-related markers such as CD4-cell counts and HIV-RNA in ART naïve and experienced patients and on the time-to-achieve CD4 cell-count >350 cells/µl in drug-naïve patients starting a first line ART-regimen.  Define the correlation between FPR obtained by population V3- genotyping and the presence of HIV-1 variants with DM/X4-tropism, detected by both Enhanced-Sensitivity Trofile phenotypic assay (ESTA) and ultra-deep V3-pyrosequencing (UDPS).

The OSCAR project helped to:

Identify new V3 genetic determinants correlated with different co-receptor usage, crucial to set up even more accurate genotypic prediction of HIV-1 tropism.  Evaluate the impact of FPR on HIV-related markers such as CD4-cell counts and HIV-RNA in ART naïve and experienced patients and on the time-to-achieve CD4 cell-count >350 cells/µl in drug-naïve patients starting a first line ART-regimen .  Define the correlation between FPR obtained by population V3- genotyping and the presence of HIV-1 variants with DM/X4-tropism, detected by both Enhanced-Sensitivity Trofile phenotypic assay (ESTA) and ultra-deep V3-pyrosequencing (UDPS).