The OSCAR project: a collaborative network throughout Italy 406 V3 - PowerPoint PPT Presentation

The OSCAR project: a collaborative network throughout Italy • 406 V3 sequences with HS Trofile results available have been collected OSCAR has been the first study aimed at evaluating the performances of genotypic tropism testing using ESTA as reference in a so large cohort of patients

The protocol for V3 sequencing set up in the framework of the OSCAR programme showed an overall efficiency of 84.2% C1 V1 C2 V2 C3 V3 C4 V4 C5 nt 1 1401 792 894 aa 1 264 298 467 S2 AS5 (nt 630-650) (nt 1292-1310) S6 (nt 682-702) AS1 (nt 1027-1047) S5 (nt 684-703) AS3bis (nt 1034-1051) Location of the V3 loop amplification and sequencing primers on HIV-1 gp120 env coding region. Primers for PCR are shown in bold black. Primers used for sequencing reaction are shown in grey

Overall Concordance between genotypic test and High Sensitivity Trofile Concordance Geno2pheno at with HS Trofile different FPR N (%/365) 296 (81.1%) G2P (5%) 286 (78.4 %) G2P (10%) Svicher et al., New Microbiologica, 2010

The OSCAR project helped to:  Identify new V3 genetic determinants correlated with different co-receptor usage, crucial to set up even more accurate genotypic prediction of HIV-1 tropism.  Evaluate the impact of FPR on HIV-related markers such as CD4-cell counts and HIV-RNA in ART naïve and experienced patients and on the time-to-achieve CD4 cell-count >350 cells/µl in drug-naïve patients starting a first line ART-regimen .  Define the correlation between FPR obtained by population V3- genotyping and the presence of HIV-1 variants with DM/X4-tropism, detected by both Enhanced-Sensitivity Trofile phenotypic assay (ESTA) and ultra-deep V3-pyrosequencing (UDPS).

The OSCAR project helped to:  Identify new V3 genetic determinants correlated with different co-receptor usage, crucial to set up even more accurate genotypic prediction of HIV-1 tropism.  Evaluate the impact of FPR on HIV-related markers such as CD4-cell counts and HIV-RNA in ART naïve and experienced patients and on the time-to-achieve CD4 cell-count >350 cells/µl in drug-naïve patients starting a first line ART-regimen.  Define the correlation between FPR obtained by population V3- genotyping and the presence of HIV-1 variants with DM/X4-tropism, detected by both Enhanced-Sensitivity Trofile phenotypic assay (ESTA) and ultra-deep V3-pyrosequencing (UDPS).

The OSCAR project helped to:  Identify new V3 genetic determinants correlated with different co-receptor usage, crucial to set up even more accurate genotypic prediction of HIV-1 tropism.  Evaluate the impact of FPR on HIV-related markers such as CD4-cell counts and HIV-RNA in ART naïve and experienced patients and on the time-to-achieve CD4 cell-count >350 cells/µl in drug-naïve patients starting a first line ART-regimen .  Define the correlation between FPR obtained by population V3- genotyping and the presence of HIV-1 variants with DM/X4-tropism, detected by both Enhanced-Sensitivity Trofile phenotypic assay (ESTA) and ultra-deep V3-pyrosequencing (UDPS).