The Effect of NaCl on the Reaction Rate of Catecholase Background - - PowerPoint PPT Presentation

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Nov 29, 2023 47 likes •151 views

The Effect of NaCl on the Reaction Rate of Catecholase Background Enzymes require a specific environment to function 1 Changes in salt concentration can disrupt form of non-covalent bonds 2 Block active sites Catecholase leads to

SLIDE 1

The Effect of NaCl on the Reaction Rate

f Catecholase

SLIDE 2

Background

❖ Enzymes require a specific environment to function 1 ❖ Changes in salt concentration can disrupt form of non-covalent bonds 2

➢ Block active sites

❖ Catecholase leads to browning of bruised or cut fruit 1 ❖ Enzymes react similarly to salt 3

SLIDE 3

Hypothesis and Prediction

★ Hypothesis

○ The amount (volume) of sodium chloride has an effect on the activity of the enzyme catecholase.

★ Prediction

○ If the amount (percent) of sodium chloride has an effect on the activity of the enzyme catecholase, then the tube with 20% NaCl will have a higher absorbance level at 400 nm.

SLIDE 4

Procedure

Prepare Test Tubes Blanking Spectrophotometer

Initial Reading 3 Final Reading 4

1 See detailed procedure below

SLIDE 5

Constants

➢ Wavelength of 400nm ➢ Amount of NaCl used, if used ➢ Drops of catecholase used - 2 ➢ Time spent in spectrophotometer - 5 min. ➢ Time enzyme was exposed to treatment

SLIDE 6

SLIDE 7

Data table

Mean of Reaction Rates Standard Deviation Control - 0% NaCl 0.0143 0.00527 IV 1 - 5% NaCl 0.0066 0.002315 IV 2 - 20% NaCl 0.0024 0.000589

SLIDE 8

Results

P-Value = 0.002 → Significant

p-value Sig or not sig? Control IV1 .024 Significant IV2 .002 Significant IV1 IV2 .231 Not significant An ANOVA test was run

SLIDE 9

Conclusion

The NaCl has an effect on the enzyme, catecholase
One possible error: not cleaning the tube before the

absorbance was taken

Future Question: how does NaCl affect other enzymes?
How much of the Na compared to the Cl affect the enzyme

activity?

SLIDE 10

Works Cited

1. University of Mary Washington Department of Biological Sciences. (n.d.). Lab Modules to Accompany BIOL 121: Observing and Investigating Biological Systems (Vol. 1). 2. "Salt Concentration Effect on Reaction Rates." UKEssays.com. 11 2013. All Answers Ltd. 10 2018 https://www.ukessays.com/essays/biology/salt-concentration-on-turnip-peroxidase-activity-biology-essay.php?vref=1 3. Greenway, H., and C. B. Osmond. “Alt Responses of Enzymes from Species Differing in Salt Tolerance.” Plant Physiology, U.S. National Library of Medicine, Feb. 1972, www.ncbi.nlm.nih.gov/pmc/articles/PMC365940/?page=3.