SLIDE 1 Thank you for joining us!
- The presentation will begin momentarily.
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mute by pressing * 6. (To unmute your phone, press * 6 again.)
- We will take questions after the
presentation.
SLIDE 2 NEPDN Advanced First Detector Training: How to Enhance Disease Diagnosis with Digital Images
Mary McKellar, NEPDN Education and Training Coordinator Kent Loeffler Cornell Department of Plant Pathology Photo Lab
SLIDE 3 Today’s Presentation
- Part 1: Disease Diagnosis and Digital Images
– How can digital images help with making a diagnosis? – How to choose the appropriate subject matter.
- Part 2: Digital Photography
– Equipment – Camera Settings – Exposure – Lighting – Post Processing
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Part 1: Disease Diagnosis Part 1: Disease Diagnosis and Digital Images and Digital Images
SLIDE 5 The Diagnostic Process: The Diagnostic Process: Sample Arrival Sample Arrival
Photo Howard F. Schwartz, www.ipmimages.org
SLIDE 6 The Diagnostic Process: The Diagnostic Process: Culture Sample Culture Sample
Photo Edward L. Barnard, www.ipmimages.org
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The Diagnostic Process: The Diagnostic Process: Microscopy Microscopy
SLIDE 8 The Diagnostic Process: The Diagnostic Process: Make Diagnosis Make Diagnosis Fusarium Wilt
Photo Howard F. Schwartz, www.ipmimages.org
SLIDE 9 Why it doesn Why it doesn’ ’t always work like t always work like that that… ….secondary organism growth .secondary organism growth
Photos NPDN First Detector Module 3: Quality and Secure Sample Submission
SLIDE 10 Why it doesn Why it doesn’ ’t always work like t always work like that that… ….wrong part of the plant .wrong part of the plant
Photo USDA Forest Service Archive, www.forestryimages.org
SLIDE 11 Why it doesn’t always work like that…. abiotic causes
Photo Joseph O’Brien, www.forestryimages.org
SLIDE 12 How do digital images help in the How do digital images help in the diagnostic process? diagnostic process?
- Digital images help by providing the
diagnostician with necessary and sufficient visual information so that a quick and accurate diagnosis can be made.
- Preliminary diagnosis may help to
speed up regulatory process. The more quickly a diagnosis can be made the more quickly containment can happen.
SLIDE 13 Taking a Good Photo: Taking a Good Photo: Subject Matter and Beyond Subject Matter and Beyond
- Focus, Focus, Focus
- Context
- Symptom Patterns and Distribution
- Big Picture to Fine Detail
- Avoid Clutter
- Scale
- Progression of Symptoms
- Multiple Angles
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Taking a Good Taking a Good Photo: Focus, Photo: Focus, Focus, Focus Focus, Focus
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That That’ ’s Better! s Better!
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Taking a Good Photo: Context Taking a Good Photo: Context
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Taking a Good Photo: Symptom Taking a Good Photo: Symptom Pattern and Distribution Pattern and Distribution
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Taking a Good Photo: Taking a Good Photo: Big Picture to Fine Details Big Picture to Fine Details
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Taking a Good Photo: Taking a Good Photo: Avoid Clutter Avoid Clutter
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Taking a Good Photo: Taking a Good Photo: Avoid Clutter Avoid Clutter
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Taking a Good Photo: Scale Taking a Good Photo: Scale
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Taking a Good Photo: Taking a Good Photo: Progression of Symptoms Progression of Symptoms
SLIDE 23 Taking a Good Photo: Taking a Good Photo: Multiple Angles Multiple Angles
Photos Joseph O’Brien, www.forestryimages.org
SLIDE 24 Submitting Your Photos Submitting Your Photos
- Contact your land grant university
diagnostic lab
– Contact info can be found at www.nepdn.org
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www.nepdn.org
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SLIDE 27 Submitting Your Photos Submitting Your Photos
Ask questions:
– Would they like digital photos to accompany your sample? – Is there specific information that should be included in the photos? – How do they want to receive the photos: via email or mail them hard copies?
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Part 2: Digital Photography Part 2: Digital Photography
SLIDE 29 Point and shoot – inexpensive but not very adaptable
Digital SLR –
Expensive but
adaptable
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SLIDE 31 Camera Settings
- White balance (WB) – Auto
- ISO – 100 to 400 (probably 400)
- Resolution – highest possible
- Format – JPEG High (Fine)
- Flash (built in) – know how to turn on/off
- Focus – know where camera focuses and
how to turn macro on/off
SLIDE 32 Exposure Settings
- P – Program, camera chooses shutter
speed and aperture
- A – Aperture priority, you choose aperture
and camera chooses shutter speed
- S – Shutter priority, you choose shutter
speed and camera chooses aperture
- M - Manual
- +/- EV – adds or subtracts exposure,
usually a button
SLIDE 33 Exposure Considerations
- Depth of Field – DOF is controlled by aperture
(f/stop) of lens. A higher f/stop (f/16) will produce greater DOF.
- Blurs – Most common problem with bad pictures
is blur caused by camera shake. Try to not use a shutter speed longer that 1/60th second.
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SLIDE 47 1 side light + reflector
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SLIDE 49 2 side lights (copy lighting)
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SLIDE 54 1 side light + reflector
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SLIDE 56 Post Processing
- iPhoto – Mac platform only
- Corel Paint Shop Pro – both platforms
- Adobe Photoshop – both platforms,
expensive but all inclusive
- Adobe Photoshop Elements – both
platforms, inexpensive, probably best for casual user
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SLIDE 80 To save for e-mail
SLIDE 81 Approximately 5x7” @ 200ppi
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SLIDE 84 From 8.3 Mb to 133.5 Kb
SLIDE 85 Digital Work Flow
- Make good exposure at High JPEG setting
- Download files to computer and save all original
files in one folder
- Open files in post-processing program and
adjust levels and color
- Save ‘fixed’ images in separate folder as JPEG
with no compression
- For e-mail change size to 5x7” @ 200ppi and
save as JPEG with ‘medium’ compression in another folder
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Thank you! Thank you! Any questions? Any questions?
