Impact of multiple disturbances on microbiological quality of - - PowerPoint PPT Presentation

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Impact of multiple disturbances on microbiological quality of - - PowerPoint PPT Presentation

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Impact of multiple disturbances on microbiological quality of urban and peri-urban lakes Presentation of the thesis project Adlade ROGUET Supervisor : Franoise LUCAS Scientific context Urban and Peri-Urban Enghien Lake Lakes Grand

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Impact of multiple disturbances on microbiological quality of urban and peri-urban lakes

Presentation of the thesis project

Adélaïde ROGUET Supervisor : Françoise LUCAS

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Scientific context

Urban and Peri-Urban Lakes

(Catherine et al., 2008) Châtillon Lake Enghien Lake Grand Marais Lake

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Ecosystem Services

(economical/recreational/educational)

Scientific context

Local scale

Anthropogenic pressures

(urban/industrial pollutions)

Global scale Climatic events changes

(rains, heatwaves)

(Poff et al., 2002; Peters et al., 2008)

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(Bolund & Hunhammar, 1999)

Lake (aquatic ecosystem)

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SLIDE 4

Lake (aquatic ecosystem)

Ecosystem Services

(economical/recreational/educational)

Scientific context

Ecological status (Ecosystem functioning) Sanitary status

(Williamson et al., 2008; Johnson et al., 2010)

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Local scale

Anthropogenic pressures

(urban/industrial pollutions)

Global scale Climatic events changes

(rains, heatwaves)

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SLIDE 5

Urban Lakes (aquatic ecosystem) Scientific context

Ecological status

Bacterial communities

(diversity/density)

Sanitary status

Indicators of faecal pollution

(E. coli/intestinal enterococci)

Environmental pathogens

(nontuberculous mycobacteria NTM) Ecological status

Bacterial communities

(diversity/density)

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Urban Lakes (aquatic ecosystem) Scientific context

Ecological status

Bacterial communities

(diversity/density)

Sanitary status

Indicators of faecal pollution

(E. coli/intestinal enterococci)

Environmental pathogens

(nontuberculous mycobacteria NTM)

Sanitary status

Indicators of faecal pollution

(E. coli/intestinal enterococci)

Environmental pathogens

(NonTuberculous Mycobacteria NTM)

Escherichia coli Mycobacterium chelonae Enterococcus faecalis

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Problematics

Identify factors controlling bacterial and pathogen populations density and diversity Modelize relative impact of global changes on microbial compartment Identify sensitive areas and factors modifying the ecological and sanitary status by modeling Optimise management practices

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PULSE Project

➛ 4 laboratories ➛ abiotic and biotic parameters monitored Material & Methods

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metals light T° conductivity TSS PAH etc. diatoms zooplankton viruses bacterial community pathogen populations etc.

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PULSE Project

➛ numerous laboratories ➛ two approaches :

PULSE Project

➛ 4 laboratories ➛ abiotic and biotic parameters monitored

larger scale (impact of land use) smaller temporal and spatial scale (seasonal changes)

Material & Methods

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✽ Larger scale : impact of land use

  • 48 lakes monitored (Ile-de-France region)
  • once a year (July-August)
  • for three consecutive years

Material & Methods

Sampling design

(Catherine et al., 2008)

Ecological status Sanitary status

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  • once a year (July-August)
  • for three consecutive years
  • 1 pooled sample per lake

Ile-de-France region

contrasted watersheds : size, land use, water management, etc.

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✽ Larger scale : impact of land use ✽ Finner scale : impact of temporal and spatial variability Material & Methods

Créteil Lake

  • monthly monitoring
  • 2 transects (horizontal/vertical)

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Sampling design Ecological status Sanitary status

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Material & Methods

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Bacterial communities

  • diversity : characterization of microbial communities
  • density : cytometry (other laboratory, ESE, Orsay France)

Sampling design Ecological status Sanitary status dHPLC (Denaturing High-Performance Liquid Chromatography) sequencing (one the three 48 lakes campaigns - Créteil Lake)

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Material & Methods

✽ Indicators of faecal pollution :

  • density : Most Probable Number
  • E. coli (NF EN ISO 9308-3)

intestinal enterococci (NF EN ISO 7899-1) ✽ Environmental pathogens : NonTuberculous Mycobacteria (NTM)

  • density : quantitative PCR hightly specific
  • structure : dHPLC

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1/10 dilutions

no dilutions

Sampling design Ecological status Sanitary status

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Results

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Only results on indicators of feacal pollution level

(6 Créteil Lake campaign + 1st 48 lakes campaign)

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Results

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Only results on indicators of feacal pollution level

(6 Créteil Lake campaign + 1st 48 lakes campaign)

[E. coli + intestinal enterococci] ➚ ➚ when

after rain events Lakes connected to the Seine river

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  • First 48 lakes campagn achieved (preparation of the next one)
  • 6 campaigns on Créteil lake
  • Development of protocols for DNA extraction
  • Harvest data for GIS
  • Creation of sampling systems for others projects

Achieved

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February 2012, Créteil Lake Assemblage of metal screen

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Thank you for your attention

Bois-le-Roi, summer 2011