Tailored made degradable synthetic polymers for 3D (bio)printing - - PowerPoint PPT Presentation

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“Tailored made degradable synthetic polymers for 3D (bio)printing”

“Maastricht, January 26th 2016”

• Prof. Christian GRANDFILS

www.ceib.ulg.ac.be C.Grandfils@ulg.ac.be

2 Specific expertises

• 1. Polymer synthesis

biodegradable polyesters ; functionalized poly(methyl)acrylate

• 2. Polymer

processing

• Hydrogels
• Film
• Dispersed materials :

micelles, nano- microparticles

• drug immobilisation
• 3. Analytical
• Solubility, Mw

determination

• Stability, releaase

kinetics study

• Particle

characterisation

• 4. In vitro toxicity
• Hemocompatibiliy
• Animal cell tox

www.ceib.ulg.ac.be

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Polymer synthesis

Particle Drug Delivery

Drug formulation

GENERAL ORGANISATION OF THE RESEARCH

VALORISATION : Patents – Close collaboration with industries – Analytical services

In vivo testing

In vitro testing

Cell therapy – Tissue engineering

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Biodegradable polyesters

• Macromolecular engineering with controlled polymerisation mechanism
• f cyclic monomers such as lactides, lactones. Novel architectures: block

graft, random star-branched copolymers

• PolymerTECH : more than 300 different polymer samples available
• Material type : thermoplastics, hydrogels, amphiphilic gels
• Batch size : from g to 100 g ; in the near future :

Kg scale facilities with reactive extrusion polymerisation

Synthetic polyelectrolytes

• Living radical polymerisation of acrylic monomers
• Stimuli-responsive polymers.
• Functionalized polymers with reactive groups for synthesis
• f bioconjugates and fluorescent labelling of biomaterials.
• Strongly adhering polymers
• Bulk polymerisation and purification in aqueous medium

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hydrophobe hydrophile

Increase the softness of the material by increasing it’s hydrophilicity

L - H - L L - H - L

50 100 150 200 250 10 20 30 40 50 60 70

Proportion molaire de PEO [%] Prise de masse [%]

15 min 24 h 1 semaine

H

Amphiphilic Biodegradable and biocompatible Hydrogels

CEIB PolymerTech

About 300 purified polyesters in stock differing in :

• Mw
• composition
• tacticity
• sequence : di- tri- block
• amphilicity

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PEO-P(D,L)LA PEO-P(L,L)LA

code Mw code Mw CK023 350-3000 CK013 350-3000 CK024 350-7500 CK014 350-7500 CK025 350-15000 CK036-6 350-12500 CK028 350-43000 CK016 350-55000 CK029 350-140000 CK194 350-140000

P(D,L)LA-PEO-P(D,L)LA P(L,L)LA-PEO-P(L,L)LA

code Mw code Mw NM008 3400-9000-3400 NM002 3800-9000-3800

Examples of polymer series

SCALE-UP

Applications of the extrusion :

• Continuous polyesters synthesis according to a reactive extrusion

process

• Polymer processing
• Polymer – drug blends

PolyLIFE – First spin-off

• F. Lombart

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Scaling-up of polymer synthesis – Spin-off : PolyLife

GMP – platform for polymer synthesis – material processing – drug encapsulation

• Scale : minimal batch size : 15 g up to Kg
• GMP equipment
• Solid / liquid feeding – control atmosphere
• Mw of PLA : up to 250,000 Da : residence time : from 1 to 25 min.

Polymer deposition by Aerosol Inject Printing technology (AJP) (in collaboration with Sirris)

Actual resolution of the polymer deposition : 40 µm

Porosity of 3D scaffolds analysed by RX µ-CT

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1 2 3 4 5 6 0.000 0.200 0.400 0.600 0.800 1.000 1.200 Pourcentage [%] Taille des pores [mm]

Pore size distribution of 3 scaffolds

51 51/52 52

51 51/52 52 51 51/52 52

Degradable textiles and stem cells.

Multifilament Monofilament Multifilament Monofilament Osteoblast cells Stem cells

Cell appearance after a 24 hours incubation with 3D textile (Confocal microscopy after fibers morphology analysis (LIVE/DEAD Kit´s labeling) Ref : Ortiz et al, J. Biomedical Mat. Research (part B), 2016.

Biocompatible biodegradable composite gel / textile for tissue engineering

New Synthetic Antidote of Heparin – PCT Patent PCT/EP2014/067081 Ch Grandfils, L. Flebus, Ch. Sevrin

Extracorporeal blood circulation

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Thanks for your attention

Welcome to Liège !

• I. Liège

A city in the heart of Europe

A city in the heart of Europe

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a French-speaking city within easy reach of the Flemish and German-speaking communities

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30 minutes from Germany and Holland 60 minutes from Brussels and Luxembourg 2:15 from Paris 3:30 from London

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A thousand-year-old historical city

A thousand-year-old historical city

A thousand-year-old historical city

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At Sart Tilman

At Sart Tilman

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