Successful STEM Majors: The Mobile DNA Laboratory Christopher M. - - PowerPoint PPT Presentation

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Successful STEM Majors: The Mobile DNA Laboratory Christopher M. - - PowerPoint PPT Presentation

Jan 22, 2023 210 likes •389 views

Preparing High School Students to be Successful STEM Majors: The Mobile DNA Laboratory Christopher M. White and Bethany Henderson-Dean 2009-2013 DNA Day Arcadia Riverdale Findlay Arlington Vanlue Liberty-Benton Cory-Rawson Van Buren

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SLIDE 1

Preparing High School Students to be Successful STEM Majors: The Mobile DNA Laboratory

Christopher M. White and Bethany Henderson-Dean

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SLIDE 2

2009-2013

DNA Day

Arcadia Arlington Cory-Rawson Findlay Liberty-Benton McComb Riverdale Vanlue Van Buren

All nine schools are in Hancock County.

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SLIDE 3

DNA Day Schedule

2009-2013

9:00 am Arrival Lecture on RFLP analysis Micropipetting exercise DNA Extraction Digestion Reaction 11:00 am Lunch Campus Tour 12:30 pm Electrophoresis/Results 2:00 pm Departure

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SLIDE 4

2013-2014

Ohio New Learning Standards: Science (2011) DNA Day program on hiatus University of Findlay Goal-Getter Awards

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SLIDE 5

2014-2015

Mobile DNA Laboratory

Applications sent to 53 schools

Wood Seneca Wyandot Allen Hardin

Continental HS

(Putnam Co.)

Shawnee HS

(Clark Co.)

Upper Scioto Valley HS

(Hardin Co.)

Wynford HS

(Wyandot Co.)

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SLIDE 6

2014-2015

Day 1

  • Fall 2014
  • CMW teaches PCR in

biology classrooms.

  • Spring 2015

Day 2

  • CMW teaches use of

micropipettors and DNA extraction in biology classrooms.

Day 3

  • April 24, 2015
  • BDHD leads

exploration of NCBI database.

  • Lunch/campus tour
  • BDHD leads

experiment on fish muscle proteins.

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SLIDE 7

2014-2015 2014-2015

Tuna Sardines Herring Ladder

200 116.3 97.4 66.3 55.4 36.5 31.0 21.5 14.4

2014-2015

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SLIDE 8

2015-2016

DNA Day program on hiatus Martha Holden Jennings Foundation

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SLIDE 9

2016-2017

Wood Seneca Wyandot Allen Hardin

  • Ft. Jennings HS

(Putnam Co.)

Hopewell-Loudon HS

(Seneca Co.)

Lima Central Catholic HS

(Allen Co.)

Mohawk HS

(Wyandot Co.)

Northwood HS

(Wood Co.)

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SLIDE 10

2016-2017

School #1

Average Pre-test score: 2.03/10 Average Post-test score: 4.62/10 Expanded Paired t-test: p<0.0001 Significant increase

School #2

Average Pre-test score: 0.51/10 Average Post-test score: 0.89/10 Expanded Paired t-test: p=0.154 Not a significant increase

School #3

Average Pre-test score: 1.00/10 Average Post-test score: 2.11/10 Expanded Paired t-test: p=0.0039 Significant increase

School #4

Average Pre-test score: 4.63/10 Average Post-test score: 5.73/10 Expanded Paired t-test: p=0.0018 Significant increase

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SLIDE 11

2016-2017

School #5

Average Pre-test score: 1.36/10 Average Post-test score: 8.00/10 Expanded Paired t-test: p<0.0001 Significant increase

Combined Scores

Average Pre-test score: 1.85/10 Average Post-test score: 2.13/10 Expanded Paired t-test: p<0.0001 Significant increase

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SLIDE 12

2016-2017

School #1

Average Pre-visit Comfort Level: 1.82/10 Average Post-visit Comfort Level: 6.00/10 Expanded Paired t-test: p<0.0001 Significant increase

School #2

Average Pre-visit Comfort Level: 1.26/10 Average Post-visit Comfort Level: 3.32/10 Expanded Paired t-test: p<0.0001 Significant increase

School #3

Average Pre-visit Comfort Level: 1.18/10 Average Post-visit Comfort Level: 5.74/10 Expanded Paired t-test: p<0.0001 Significant increase

School #4

Average Pre-visit Comfort Level: 2.41/10 Average Post-visit Comfort Level: 7.66/10 Expanded Paired t-test: p=0.0018 Significant increase

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SLIDE 13

2016-2017

School #5

Average Pre-visit Comfort Level: 1.36/10 Average Post-test score: 8.00/10 Expanded Paired t-test: p<0.0001 Significant increase

Combined Scores

Average Pre-visit Comfort Level: 1.42/10 Average Post-visit Comfort Level: 5.38/10 Expanded Paired t-test: p<0.0001 Significant increase

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Conclusions/Future Plans

  • Students understanding of PCR and DNA technologies

increased significantly in all but one school measured.

  • Students self-reported comfort level with the use of

micropipettors increased significantly in each school measured.

  • Teachers believe this program is an enormous benefit to

their students. (Additional evidence not presented here)

  • Application for an additional year of funding from

Martha Holden Jennings Foundation in the works.

  • Modifications to the program will coincide with

advice from teacher evaluations. (Additional

evidence not provided here)

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Acknowledgements

  • Dr. Michael Edelbrock for the original idea in 2009
  • Funding Sources:
  • University of Findlay Biology Department
  • University of Findlay Goal-Getter Program
  • Martha Holden Jennings Foundation
  • Teachers who allowed us to have a ball teaching their students:
  • Amanda Baker (Mohawk HS)
  • Tammy Baker (Continental HS)
  • Jeff Jostpille (Ft. Jennings HS)
  • Kristin Meeks (Shawnee HS)
  • Norma Perrone (Upper Scioto Valley HS)
  • Deb Schenk (Lima Central Catholic HS)
  • David Shumaker (Northwood HS)
  • Kelly Wheeler (Wynford HS)
  • Carla Young (Hopewell-Loudon HS)
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SLIDE 16

Questions?