spICP-MS tier 1 method People behind the experiments Anna - PowerPoint PPT Presentation

spICP-MS – tier 1 method “People behind the experiments” Anna Katarzyna Undas (RIKILT) NanoDefine Outreach Event Brussels, September 2017 NanoDefine is funded by the European Community's Seventh Framework Programme under Grant Agreement-604347

RIKILT (Wageningen University & Research) is your innovative partner for safe and authentic food. We are specialized in (forensic) measurements, perform top level research and by arrangement 24/7 available in case of emergencies Wageningen Expertise areas: • Food fraud and Composition • Effects of substances on humans and animals • Measuring and detecting substances • Safe food production • Training & Consultancy

RIKILT (Wageningen University & Research) RIKILT coordinated EU-projects NanoLyse and NanoDefine and was a research partner in many more EU projects focusing on nanotechnology Wageningen

NanoDefine project – people behind experiments

spICP-MS – EU nanomaterial definition Nanomaterial EU definition: “A natural, incidental or manufactured material containing particles, in an unbound state or as an aggregate or as an agglomerate and where, for 50 % or more of the particles in the number size distribution, one or more external dimensions is in the size range 1 nm - 100 nm .” • Determination of size • Determination of size distribution • Number based concentration

spICP-MS method

spICP-MS – method validation Validation based on: • IUPAC “Harmonized Guidelines for Single - Laboratory Validation of Analytical Methods” • Eurachem Guide “Fitness for Purpose of Analytical Methods” • Commision Decision 2002/657/EC 5 days, n=3 (LOD,LOQ, n=5) TiO 2 in sunscreen Al 2 O 3 in toothpaste 20 – 500 nm 48 – 500 nm Working range 2.4 A , 8.0 B , 19.9 C 5.3 A , 5.8 B , 2.3 C Repeatability (RSD r ) [%] Intermediate precision (RSD RL ) [%] 4.0 A , 16.5 B , 21.3 C 15 A , 24 B , 29 C LOD LODs 20 nm LODs 48 nm LODc 12 mg/kg prod. (10 peaks) LODc 61 mg/kg prod. (10 peaks) LOQ LOQs 22 nm LOQs 70 nm LOQc 30 mg/kg prod. (30 peaks) LOQc 180 mg/kg prod. (30 peaks) Measurement uncertainty [%] 5 A , 25 B , 33 C 17 A , 25 B , 33 C A particle diameter, B particle number conc., C particle mass conc.

Analysis of commercially available toothpaste Product Median size [nm] a 154 b 126 c 143 d 140 e 123 f 110 g 143 h 106

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www.NanoDefine.eu This project has received funding from the European Union’s Seventh Programme for research, technological development and demonstration under grant agreement No 604347