SELF-CLEARING IMPLANTABLE BIOSENSORS FOR NEURODEGENERATION RESEARCH - PowerPoint PPT Presentation

Towards SELF-CLEARING IMPLANTABLE BIOSENSORS FOR NEURODEGENERATION RESEARCH Hyowon “Hugh” Lee Assistant Professor Weldon School of Biomedical Engineering Purdue University

Acknowledgements first… Grad Students: Qi Yang Hyunsu Park Tran Nguyen James Nolan Angel Enriquez Haesoo Moon Jongcheon Lim Jian Xu – Postdoc Jinjia Xu – Postdoc Collaborators: Riyi Shi, Herman Sintim, Bryan Boudouris

State-of-the-art in implantable sensors Abbott Freestyle Libre CGM: 14 days G6, Dexcom CGM: 10 days LICOX Brain Tissue Oxygenation Monitor Integration Neurosciences: 7-14 days Eversense, Senseonics: 90 days 3

Spinal cord injury statistics and facts 2.5 millions worldwide Demographic 75 years 0 years 347,000 29-42 in the US Long term complication Annual national cost Primary injury Secondary injury Normal spinal cord Severe axon Demyelination $9.7 billions Exctitotoxicity and itoto Spinal cord neuron Immune system cell oxidative damage Astrocyte Cellular debris Apotosis Activated microglia Resting microglia Reactive astrocyte

Nanocomposite-based flexible biosensor 50 µm 100 µm Nguyen et al., Biosens. Bioelectron. 2019 5

Fabrication, in vitro, ex vivo tests a. PtNPs-MWCNT-PEDOT:PSS-Na fj on-GluOx 1600 MWCNT-PEDOT:PSS-Na fj on-GluOx 200 µ M PEDOT:PSS-Na fj on-GluOx 1400 Current Density (nA/mm 2 ) 1200 1000 800 200 µ M 600 50 µ M 400 Device in the 50 µ M 50 µ M 200 spinal cord 0 c. 1200 1300 1400 1500 1600 1700 1800 Time (s) Nguyen et al., Biosens. Bioelectron. 2019 6

Reliability issues à opportunity! Prevent & Disrupt Biofouling! Strategy: Use Active and Passive Approaches

Active anti-biofouling strategy * * 𝜚 H = 16kA/m H = 0 * Yang et al., S&A:B, 2018 𝜚 = 𝑊 $ 𝑁𝐼𝑡𝑗𝑜 + − 𝜚 /k /012 Dynamic responses of microactuators 𝐽 = 𝑥𝑢 = 𝑙 456$ = 𝐹𝐽 𝑀 , Removing blood clot obstruction 12 Large amplitude actuation for self-cleaning Moving backwards Moving forward

Towards self-clearing biosensors Unpublished

Questions?