Realization of new lactic ferments Mounir Benkoulouche 1 , Floriane - - PowerPoint PPT Presentation

▶
realization of new lactic ferments
SMART_READER_LITE
LIVE PREVIEW

Realization of new lactic ferments Mounir Benkoulouche 1 , Floriane - - PowerPoint PPT Presentation

Apr 24, 2023 222 likes •490 views

Realization of new lactic ferments Mounir Benkoulouche 1 , Floriane Bretheau 1 , Jean-Rmy Brossier 1 , Laurianne Daniel 1 , Clment Delestre 1 , Jeveta Ghanty 1 , Jonathan Melius 1 , Ccile Qur 1 ,Alexandre Trichies 1 , Mava Veyssiere 1

slide-1
SLIDE 1

Realization of new lactic ferments

Mounir Benkoulouche1, Floriane Bretheau1, Jean-Rémy Brossier1, Laurianne Daniel1, Clément Delestre1, Jeveta Ghanty1, Jonathan Melius1, Cécile Quéré1,Alexandre Trichies1, Maëva Veyssiere1 Denis Dupuy2, Marie Beurton-Aimar2

(1) with an equal contribution to this work (2) Instructors

slide-2
SLIDE 2

Team Presentation Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

Floriane Alexandre Mounir Jean-Rémy Jeveta and others who were unable to come …

  • Team Bordeaux 2013
slide-3
SLIDE 3

Introduction Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

  • Vector for lactic acid bacteria in the digestive tract
  • Ideal support to begin an iGEM project
  • Probiotics are commonly consumed as part of fermented foods
  • Example of probiotic : Kefir
  • The yoghurt's choice

kombuchakefir.com

slide-4
SLIDE 4

Introduction Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

  • Probiotic effects on metabolism and health

Kefir – a complex probiotic Edward R. Farnworth

slide-5
SLIDE 5

Introduction Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

  • Production of flavoured yogurts with beneficial effects on the
  • rganisms and medical properties without using chemical

substances

  • Commercial interest
  • Interest for consumer
  • Our project goals
slide-6
SLIDE 6

Introduction Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

  • Preparation of Yogurts :
  • Two species of bacteria
  • Streptococcus growth
  • Lactobacillus growth
  • Fermentation : 3 to 4 hours
  • nly
  • Extracellular polysaccharides
slide-7
SLIDE 7

Material and methods Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

Make competent E.coli Test of the red biobrick

  • n E.coli

Red colonies put in a liquid culture Promotor HlbA added to the plasmid Insertion in Lactobacillus

slide-8
SLIDE 8

Project: Dyes and Flavour Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

  • Raspberry dye :
  • One of the most expensive

dye

  • Produce directly in the

yogurt

  • Two enzymes are necessary
  • Two biobricks needed
slide-9
SLIDE 9

Project: Dyes and Flavour BBa_K1148000 crtE (phlbA) BBa_K1148001 crtB (phlbA) BBa_K1148002 crtI (phlbA) BBa_K1148003 crtE + crtB + crtI (phlbA)

  • Lycopene: red

geranylgeranyl pyrophosphate synthase phytoene synthase phytoene desaturase

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-10
SLIDE 10

Project: Dyes and Flavour BBa_K1148004 : crtY under hlbA promoter (phlbA) BBa_K1148005 : crtZ under hlbA promoter (phlbA) lycopene beta-cyclase beta-carotene 3-hydroxylase

  • β-carotene and zeaxanthin: orange and yellow

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

crtY crtZ

slide-11
SLIDE 11

Project: Dyes and Flavour BBa_K1148011 : the gene for monoterpenyl-diphosphatase under hlbA promoter (phlbA)

  • Geraniol: rose flavour

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-12
SLIDE 12

Project: Dyes and Flavour BBa_K1148010 : TPS10 under hlbA promoter (phlbA) myrcene/ocimene synthase

  • Myrcene: lavender flavour

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-13
SLIDE 13

Project: Dyes and Flavour BBa_K1148008 : the gene for limonene synthase 1 under hlbA promoter (phlbA)

  • Limonene : citrus flavour

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-14
SLIDE 14

Project: Dyes and Flavour BBa_K1148009 : TPC-CIN under hlbA promoter (phlbA) 1,8-cineole synthase

  • α-terpineol: pine flavour

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-15
SLIDE 15

Project: Resveratrol

  • What is resveratrol ?

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-16
SLIDE 16

Project: Resveratrol The French paradox Anti cancer properties

  • Why resveratrol?

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-17
SLIDE 17

Project: Resveratrol

  • Resveratrol molecule

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-18
SLIDE 18

Project: Resveratrol

  • Enzymatic pathway

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-19
SLIDE 19

Project: Resveratrol

  • What we wanted to do and how?
  • Include resveratrol pathway in Lactobacillus.

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-20
SLIDE 20

Human Practice

  • The Virtual Lab
  • The BioGame
  • iGEM House

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-21
SLIDE 21

Human Practice: The Virtual Lab

  • The Virtual Lab
  • Virtual Lab = a dedicated Online Information Management System to help

Igem teams to visualize the progress of their project in real time and share with their off-site team members.

  • Thanks to our bio-computer engineer students :
  • Maëva VEYSSIERE
  • Jonathan MELIUS
  • Clément DELESTRE

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-22
SLIDE 22

Human Practice: The BioGame

  • The BioGame
  • As for biologists as for non-initiates
  • Goal: Get your project parts
  • How: Answer Biology questions

Who said id Biology

  • logy was boring

ring ? … … play ay Biology

  • logy and have

ve FUN !

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-23
SLIDE 23

Human Practice: iGEM House

  • iGEM House
  • Synthetic Biology and House of the future.

=> A house in which many iGEM projects would be every days used tools.

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-24
SLIDE 24

Conclusion Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

  • Colors and flavors

Design of new potential biobricks  Manage to end these constructions for a transformation in E.coli then L.bacillus

  • Resveratrol

Extraction of RNA from grapevines done  Biobrick construction to produce resveratrol

  • Human practice
slide-25
SLIDE 25

Thanks

  • Denis Dupuy1
  • Marie Beurton-Aimar1
  • Cécile Quéré2

(1) Instructors (2) Doctoral student

Team Presentation Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion

iGEM Bordeaux Team 2013 – Super Yoghurt

slide-26
SLIDE 26