Realization of new lactic ferments Mounir Benkoulouche 1 , Floriane - PowerPoint PPT Presentation

Realization of new lactic ferments Mounir Benkoulouche 1 , Floriane Bretheau 1 , Jean-Rémy Brossier 1 , Laurianne Daniel 1 , Clément Delestre 1 , Jeveta Ghanty 1 , Jonathan Melius 1 , Cécile Quéré 1 ,Alexandre Trichies 1 , Maëva Veyssiere 1 Denis Dupuy 2 , Marie Beurton-Aimar 2 (1) with an equal contribution to this work (2) Instructors

Team Team Presentation Presentation  Team Bordeaux 2013 Introduction Material and Methods Project: Dyes and Floriane Jeveta Flavour Project: Resveratrol Human Jean-Rémy Alexandre Mounir Practice and others who were unable to come … Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Introduction Presentation  The yoghurt's choice Introduction Material and • Vector for lactic acid bacteria in the digestive tract Methods • Ideal support to begin an iGEM project Project: Dyes and • Probiotics are commonly consumed as part of fermented foods Flavour • Example of probiotic : Kefir Project: Resveratrol Human Practice kombuchakefir.com Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Introduction Presentation  Probiotic effects on metabolism and health Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Kefir – a complex probiotic Edward R. Farnworth Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Introduction Presentation  Our project goals Introduction Material and • Production of flavoured yogurts with beneficial effects on the Methods organisms and medical properties without using chemical substances Project: Dyes and • Commercial interest Flavour • Interest for consumer Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Introduction Presentation  Preparation of Yogurts : Introduction Material and Methods • Two species of bacteria • Streptococcus growth Project: Dyes and • Lactobacillus growth Flavour • Fermentation : 3 to 4 hours only Project: Resveratrol • Extracellular polysaccharides Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Material and methods Team Presentation Introduction Material and Methods Insertion in Lactobacillus Promotor HlbA Project: added to the Dyes and Red colonies plasmid Flavour put in a liquid culture Test of the red biobrick Project: on E.coli Resveratrol Make competent E.coli Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Dyes and Flavour Presentation  Raspberry dye : Introduction Material and • One of the most expensive Methods dye • Produce directly in the Project: yogurt Dyes and Flavour • Two enzymes are necessary • Two biobricks needed Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Dyes and Flavour Presentation  Lycopene: red Introduction Material and Methods geranylgeranyl pyrophosphate synthase Project: Dyes and phytoene synthase Flavour phytoene desaturase Project: Resveratrol BBa_K1148000 crtE (phlbA) BBa_K1148001 crtB (phlbA) BBa_K1148002 crtI (phlbA) Human BBa_K1148003 crtE + crtB + crtI (phlbA) Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Dyes and Flavour Presentation  β -carotene and zeaxanthin: orange and yellow Introduction BBa_K1148004 : crtY under hlbA promoter (phlbA) Material and BBa_K1148005 : crtZ under hlbA promoter (phlbA) Methods Project: lycopene beta-cyclase Dyes and crtY Flavour beta-carotene Project: 3-hydroxylase crtZ Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Dyes and Flavour Presentation  Geraniol: rose flavour Introduction Material and Methods BBa_K1148011 : the gene for monoterpenyl-diphosphatase Project: under hlbA promoter (phlbA) Dyes and Flavour Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Dyes and Flavour Presentation  Myrcene: lavender flavour Introduction Material and Methods BBa_K1148010 : TPS10 under hlbA promoter (phlbA) Project: myrcene/ocimene synthase Dyes and Flavour Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Dyes and Flavour Presentation  Limonene : citrus flavour Introduction Material and Methods BBa_K1148008 : the gene for limonene synthase 1 under hlbA promoter (phlbA) Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Dyes and Flavour Presentation  α -terpineol: pine flavour Introduction Material and Methods BBa_K1148009 : TPC-CIN under hlbA promoter (phlbA) Project: 1,8-cineole synthase Dyes and Flavour Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Resveratrol Presentation  What is resveratrol ? Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Resveratrol Presentation  Why resveratrol? Introduction Material and Methods Project: Dyes and Flavour Anti cancer properties Project: Resveratrol Human The French paradox Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Resveratrol Presentation  Resveratrol molecule Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Resveratrol Presentation  Enzymatic pathway Introduction Material and Methods Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Project: Resveratrol Presentation Introduction Material and Methods  What we wanted to do and how? Project: • Include resveratrol pathway in Lactobacillus. Dyes and Flavour Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Human Practice Presentation Introduction Material and Methods  The Virtual Lab Project:  The BioGame Dyes and Flavour  iGEM House Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Human Practice: The Virtual Lab Presentation Introduction  The Virtual Lab Material and Methods Project: Dyes and Flavour • Virtual Lab = a dedicated Online Information Management System to help Igem teams to visualize the progress of their project in real time and share with their off-site team members. Project: Resveratrol • Thanks to our bio-computer engineer students : - Maëva VEYSSIERE Human - Jonathan MELIUS Practice - Clément DELESTRE Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Human Practice: The BioGame Presentation Introduction  The BioGame Who said id Biology ology was boring ring ? … Material and Methods Project: • As for biologists as for non-initiates • Dyes and Goal: Get your project parts • How: Answer Biology questions Flavour Project: Resveratrol Human Practice … play ay Biology ology and have ve FUN ! Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Human Practice: iGEM House Presentation Introduction  iGEM House Material and • Synthetic Biology and House of the futur e. Methods => A house in which many iGEM projects would be every days used tools. Project: Dyes and Flavour Project: Resveratrol Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Conclusion Presentation Introduction Material and  Methods Colors and flavors Design of new potential biobricks  Manage to end these constructions for a transformation in E.coli then L.bacillus Project: Dyes and  Resveratrol Flavour Extraction of RNA from grapevines done  Biobrick construction to produce resveratrol Project:  Resveratrol Human practice Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt

Team Thanks Presentation Introduction Material and Methods Project: - Denis Dupuy 1 Dyes and - Marie Beurton-Aimar 1 Flavour - Cécile Quéré 2 Project: Resveratrol (1) Instructors (2) Doctoral student Human Practice Conclusion iGEM Bordeaux Team 2013 – Super Yoghurt