p Coumaric acid inhibits anaerobic digestion of lignocellulose, - - PowerPoint PPT Presentation

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p Coumaric acid inhibits anaerobic digestion of lignocellulose, - - PowerPoint PPT Presentation

Aug 19, 2023 46 likes •216 views

p Coumaric acid inhibits anaerobic digestion of lignocellulose, laccase detoxifies Michel Schroyen, Stijn Van Hulle, Han Vervaeren & Katleen Raes Overview (1) Lignocellulose Pretreatment Anaerobic Agricultural waste Thermal digestion

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SLIDE 1

p‐Coumaric acid inhibits anaerobic digestion of lignocellulose, laccase detoxifies

Michel Schroyen, Stijn Van Hulle, Han Vervaeren & Katleen Raes

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SLIDE 2

Overview (1)

Lignocellulose

Agricultural waste Forestry residue

Pretreatment

Thermal Chemical Biological

Anaerobic digestion

2

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SLIDE 3

Lignocellulose

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“You can make anything out of lignin, except money.”

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SLIDE 4

80 130 180 230 280 330 5 10 15 20 BMP after 30 days (Nl/kgVS) Lignin (g/100g)

Lignin & BMP

Substrate Lignin % BMP Ensilage 1 308 Corn Stover 4,5 231 Wheat Straw 6 226 Flax Straw 8,6 232 Hemp Straw 9,2 240 Miscanthus 12 140 Willow 17 92 Schroyen et al. 2015 (Bioresource Technology)

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SLIDE 5

Lignocellulose Lignin Degradation = Lignin degradation

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Pretreatment

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SLIDE 6

TPC & BMP

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5 10 15 20 25 30 50 100 150 200 250 300 Hydrolysis rate (Nl/kgVS/d) Phenolic compounds (mg/l)

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SLIDE 7

Overview (2)

Lignocellulose

Agricultural waste Forestry residue

Pretreatment

Thermal Chemical

Anaerobic digestion

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Inhibitors

Phenolic compounds

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SLIDE 8

Experimental Setup (1)

S

Miscanthus / Hemp straw

PC

p‐Coumaric acid 0 – 2000 mg/l

AD

BMP – determination

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SLIDE 9
  • The hydrolysis rate during anaerobic digestion of

miscanthus is reduced by p‐coumaric acid

Inhibition anaerobic digestion Miscanthus Hemp straw

S PC AD

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SLIDE 10

Experimental Setup (2)

S

Miscanthus / Hemp straw

PC

p‐Coumaric acid 0 – 500 mg/l

E

Laccase / Versatile peroxidase

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SLIDE 11
  • Laccase diminishes the concentration of p‐coumaric

acid

Detoxifying effect of enzymes Laccase Versatile Peroxidase

S PC E

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SLIDE 12
  • 500 mg/l inhibits versatile peroxidase completely

Impact on the enzymes

‐20 20 40 60 80 100 120 Uninhibited activity Laccase Versatile peroxidase Relative enzyme activity

S PC E

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SLIDE 13

Overview (3)

Lignocellulose

Agricultural waste Forestry residue

Pretreatment

Thermal Chemical

Anaerobic digestion

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Detoxification

Laccase

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SLIDE 14

Experimental Setup (3)

S

Miscanthus / Hemp straw

PC

p‐Coumaric acid 0 – 2000 mg/l

E AD

BMP ‐ determination

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SLIDE 15
  • A laccase detoxifying step has a positive effect on

hydrolysis as well as on total biogas production

Connecting the dots

S PC E AD

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SLIDE 16

Conclusion

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  • Lignin has a negative impact on BMP
  • Increase of phenolic compounds inhibits

anaerobic digestion of miscanthus

  • Versatile peroxidase is inhibited
  • Laccase detoxifies and can be used as an extra

step in the production process

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SLIDE 17

Thank you!

Contact details: michel.schroyen@ugent.be