ANAEROBIC CO-DIGESTION OF OLEIC ACID AND WHEY PROTEIN: THE ROLE OF - - PowerPoint PPT Presentation

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ANAEROBIC CO-DIGESTION OF OLEIC ACID AND WHEY PROTEIN: THE ROLE OF - - PowerPoint PPT Presentation

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DEMOCRITUS UNIVERSITY OF THRACE DEPARTMENT OF ENVIRONMENTAL ENGINEERING Laboratory of Wastewater Management and Treatment T echnologies ANAEROBIC CO-DIGESTION OF OLEIC ACID AND WHEY PROTEIN: THE ROLE OF EMULSIFICATION A. Eftaxias, C.

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ANAEROBIC CO-DIGESTION OF OLEIC ACID AND WHEY PROTEIN: THE ROLE OF EMULSIFICATION

  • A. Eftaxias, C. Michailidis, V. Diamantis, A. Aivasidis

Email: alexeftaxias@gmail.com, bdiamant@env.duth.gr Heraklion, June 2019

DEMOCRITUS UNIVERSITY OF THRACE

DEPARTMENT OF ENVIRONMENTAL ENGINEERING Laboratory of Wastewater Management and Treatment T echnologies

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SLIDE 2

Anaerobic digestion of FOG

  • Fats, Oils and Grease (FOG) are preferred co-substrate for

Anaerobic Digestion.

  • High theoretical biomethane yield (1m3 CH4 kg−1).
  • FOG is a desirable substrate to enhance biomethane

production.

  • Increase methane yield up to 250%.
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SLIDE 3

Oleic acid

  • Oleic acid is the most widespread

unsaturated fatty acid in nature.

  • It is suitable for biogas production.
  • Ιs often responsible for process inhibition,

due its high toxicity even at low concentrations.

  • In this study, anaerobic co-digestion of
  • leic acid and whey protein was examined

in continuous (batch-fed) stirred tank reactors.

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SLIDE 4

Anaerobic Digesters Design & Operation

Parameters Digester type CSTR Digester volume 2L T emperature Mesophilic ~ 38

  • C

Mixing velocity 100 rpm Feeding type Fed – batch (daily) MLSS 10.2 ± 2.5 g/L NH4-N 2.5 ± 0.2 g/L Micro- nutrients Eftaxias et al. 2018

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SLIDE 5

Preparation of proteinaceous solution

  • 20gr/L whey protein
  • Pre-heated at 75oC
  • High shear 6000rpm
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SLIDE 6

Preparation of both mixtures

Emulsifjed mixture with

  • leic acid
  • Mixing 5g/L of oleic acid

when proteinaceous solution was performed. No emulsifjed mixture with

  • leic acid
  • The proteinaceus solution

and oleic acid were added separately into the digester

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SLIDE 7

Waste Characteristics

Proteinaceous solution PARAMETER VALUE

pH

  • 6.02 ±

0.04 EC mScm-1 890.5 ± 40.4 COD prot gL-1 28.45 ± 2.36

Emulsifjcation (5gOleic/L)

PARAMETER VALUE

pH

  • 5.78 ±

0.21 EC mScm-1 756.1 ± 65.7 COD total gL-1 41.03 ± 2.77

PARAMETER VALUE

pH

  • 6.20 ±

0.29 EC mScm-1 859.1 ± 104 COD prot gL-1 28.4 ± 2.2 COD oleic gL-1 12.85 ± 0.2 COD total gL-1 40.45 ±

No - Emulsifjcation

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SLIDE 8

Results

  • Emulsifjed
  • No-emulsifjed
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SLIDE 9

Results

  • Emulsifjed
  • No-emulsifjed
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SLIDE 10

Results

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SLIDE 11

Results

  • Emulsifjed
  • No-emulsifjed
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SLIDE 12

Results – Maximum uptake rate for degrading LCFA

  • Emulsifjed (km_fa =

6kg/kgd)

  • No-emulsifjed (km_fa =

1.41kg/kgd)

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SLIDE 13

Conclusions

  • The results of this study demonstrate the importance of emulsifjcation

pre-treatment for high-rate anaerobic treatment of oleic acid based effmuents.

  • Without emulsifjcation procedure the anaerobic digestion of oleic acid was

unstable, presenting high foaming incidents and severe accumulation.

  • Oleic acid solubility plays a critical role on the anaerobic digestion

process.

  • Maximum uptake rate for LCFA degrading microorganisms (km_fa) base on

ADM1 modelling was 6 kg/kgd and 1.41 kg/kd for emulsifjed and non emulsifjed oleic acid respectively.

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SLIDE 14

Acknowledgements The research was supported by the General Secretariat for Research and T echnology (GSRT) and the Hellenic Foundation for Research and Innovation (HFRI).

Thank you