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Operation Debug
NCT NCTU_F U_For
- rmosa
mosa
Harmful ful Insect ect Eliminatio mination n Force Headquarters: National Chiao Tung University, Hsinchu, Taiwan
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Operation Debug NCT NCTU_F U_For ormosa mosa Harmful ful - - PowerPoint PPT Presentation
Operation Debug NCT NCTU_F U_For ormosa mosa Harmful ful - - PowerPoint PPT Presentation
Operation Debug NCT NCTU_F U_For ormosa mosa Harmful ful Insect ect Eliminatio mination n Force Headquarters: National Chiao Tung University, Hsinchu, Taiwan 1 Motivation | Insect Damage Current Solution: Pesticide 2 3 Target | Ori
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Insect Damage Current Solution: Pesticide
2
Motivation|
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3
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Target |
Ori Orien ental tal Le Leafw afwor
- rm Moth
Moth (Spo Spodopter doptera li litur tura) Ag Agricultur ricultural al dama amage ge to to
- ver 40
er 40 f famili amilies es of
- f plants
plants
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Tacti ctical cal
Plan A : Biologically Synthesized Pheromone Traps
Plan an
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Regulatory Role of PBAN in Sex Pheromone Biosynthesis of Heliothine Moths ( Russell Jurenka, and Ada Rafaeli, Oct 10 2011 ) SLIDE 7
Tacti ctical cal
Plan B :
Plan an |
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PBAN
(Pheromone Biosynthesis Activating Neuropeptide) Infiltration
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Tacti ctical cal Plan an | Gland Gland
Rec ecep eptor tor PB PBAN AN
Pher Pheromone
- mone
Bi Biosy
- synth
nthesis esis Acti Activa vated ted
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PBAN
Specialties
(1) Peptide (2) Small (3) Stable (4) Specific
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Operation Detail |
Phe Pheromo
- mone
ne
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Biob Biobric rick
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Biobrick |
RBS RBS
PB PBAN AN
Ter er Pcons
cons
PB PBAN AN PB PBAN AN-SL SL
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Results – Making PBAN|
Orien Orienta tal l Le Leafw afwor
- rm Moth
Moth (Spodopte tera litur itura)
PCR
750 bp 500 bp
SDS-PAGE
Control
25 kDa 11 kDa 3.7 kDa
BBa_ BBa_K14151 K1415105 05
11 RBS RBS
PB PBAN AN-SL SL Pcons
cons
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Results-Making PBAN| BBa_K14 BBa_K1415 1520 205
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PBAN-SL
Pcons
BFP
RBS RBS RBS RBS Ter er
Control PBAN-SL
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Ca2+
Adenylate Cyclase
PBAN receptor Calmodulin
PB PBAN AN
G protein
Mechanism |
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Centrifuge
Add Buffer Pour out LB
Vortex
Mash Dilute Autoclave
Obtain Bio-safe PBAN solution
E.coli contain PBAN
Sonicator
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PBAN Cott Cotton
- n
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Con Contr trol
- l
( W ( With ithou
- ut
t PB PBAN AN ) With ith PB PBAN AN
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- |
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Parts of Device |
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Operational Process|
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Operational Process |
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Modeling|
24 Device for light preference
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Result - PCR |
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SL BM MB AI LD HAH AS SI AA
750 bp MB AI LD SL HAH AS SI AA 500 bp 250 bp BM
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Result - SDS PAGE |
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Control BM
AA LD AS
25 kDa 11 kDa
AI MB HAH SI Control
2-4 kDa
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Control SL BM MB AI LD HAH AS SI AA
PBAN-SL
Pcons
BFP
RBS RBS RBS RBS Ter er
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Hu Human man Pr Prac actic tice
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Cooperation|
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Industry Collaboration
Team Exchange
iGEM Promoting
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Organic Farm |
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iGEM Conference |
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Bio-camp|
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Green Market |
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Achievements|
New basic and composite BioBrick parts
We converted 9 PBANs into standard parts and constructed 27 new BioBricks.
Successfully resolving insect damage
We developed a brand new control system and a device for its application.
Real application in industry
Organic farms are currently using our new system and loving it.
Team Meetups
We held an international conference with 22 iGEM teams from China and Taiwan.
Help any registered iGEM team from another school
We assisted TCU Taiwan iGEM Team with their modeling.
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Ackno knowledg ledgement ement
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Ins Instr truc ucto tors: s:
- Prof. Yan-Hwa Wu
- Prof. Yuh-Jyh Jong
- Prof. Hsien-Da Huang
- Prof. Shin-Ying Ho
- Prof. Hsiao-Ching Lee
- Prof. Wen-Liang Chen
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