of MALDI Process by Superconducting Ion Detectors Super-I RIIF, AIST - PDF document

Dark matter in our body Fragmentation Analysis � of MALDI Process by Superconducting Ion Detectors Super-I � RIIF, AIST � M. Ohkubo, Y. Shigeri, T. Kinumi, N. Saito, M. Ukibe,Y. E. Chen, A. Kushino, A. Kurokawa, and S. Ichimura 1 � Post-genome research Super-I � RIIF, AIST � � � Junk DNA � � Repeated sequence � � Epigenetic factor nucleus � � … Golgi complex Mitochondria Which protein, Ribosomes Where, 15 � m When, How much 2 �

？ Time-of-flight mass spectrometer Super-I � RIIF, AIST � N 2 laser HV Mass spectrum Ion intensity MCP Time of flight ( m/z ) MALDI 100% STJ, in principle Detection efficiency ~3% for BSA 66.4 kDa 0 Mass of molecule 3 � !"#$%&$'()( 1 MDa Super-I � RIIF, AIST � monomer 4 �

Evidence of 100% detection efficiency Super-I � RIIF, AIST � 1 2 3 4 5 5 � LTD in TOF-MS Super-I � RIIF, AIST � • � STJ, NIS, TES have been applied to MS. (PSI, NIST, LLNL, Comet, …) • � MS community may have impression, very small, slow response, very low temperature, not suitable for top down proteomics. (Intact ion detection is not enough.) 6 �

Our target Super-I � RIIF, AIST � • � * ns time resolution • � 100 pixel array • � Fragmentation analysis (PSD) by kinetic energy (MS/MS) • � Trajectory analysis after dissociation for ionic and neutral molecules (MS/MS) 7 � Fragmentation in top down proteomics Super-I � RIIF, AIST � B. Spengler, JSM 32 1019 (1997) Detector Ion source E=E 1 +E 2 E E 1 E 2 8 �

Fragmentation in MALDI ion source Super-I � RIIF, AIST � &'()* ! +,-)*.$"/ %!! 25 m/Z=(1.363 x t - 5.1161)2 BSA++ + $"! 20 BSA Kinetic energy (512 ch.) 66430 $!! BSA+ 15 Ion count #"! 10 #!! BSA++ 5 "! ! 0 ! "! #!! #"! $!! $"! 0 20000 40000 60000 80000 !"#$%&'( m/z &'()* ! +,-)*.$%/ 100 BSA++ m/Z=(1.363 x t - 5.1161)2 BSA+ 90 $!! 66430 80 Kinetic energy (512 ch.) 70 BSA+ BSA++ 60 Ion count #!! 50 40 30 "!! 20 10 ! 0 ! %! "!! "%! #!! #%! 0 20000 40000 60000 80000 9 � m/z !"#$%&'(