ODR-10 expressed in human HEK293 cells Ado pte d fro m Y. Zha ng , - - PowerPoint PPT Presentation

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ODR-10 expressed in human HEK293 cells Ado pte d fro m Y. Zha ng , - - PowerPoint PPT Presentation

Mar 30, 2024 558 likes •848 views

- A Synthe tic Pa thwa y fo r Pe st Co ntro l ODR-10 expressed in human HEK293 cells Ado pte d fro m Y. Zha ng , e t a l, 1997. Oute r Me mbr ane Inne r GDP G GT P G G G G MAPK G pa thwa y F US1 F AR1 Ce ll Ma

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SLIDE 1
  • A Synthe tic Pa thwa y fo r Pe st Co ntro l
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SLIDE 2
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SLIDE 3
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SLIDE 4
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SLIDE 5
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SLIDE 6
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SLIDE 7

ODR-10 expressed in human HEK293 cells

Ado pte d fro m Y. Zha ng , e t a l, 1997.

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SLIDE 8

Gα

G β

G γ

F AR1 GT P

Oute r Me mbr ane Inne r

MAPK pa thwa y

Gα

G β

G γ

GDP

F US1 Ce ll c yc le a rre st Ma ting

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SLIDE 9

Rat RI7 odor ant r e c e ptor N N C C ODR- 10 r e c e ptor

1 2 3 4 5 6 7 Oute r Ce llular me mbr ane Inne r

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SLIDE 10

Gpa1 Ga olf Gpa1 Gαolf

G β

G γ

F AR1 GT P

MAPK pa thwa y

G β

G γ

GDP

F US1 Ce ll c yc le a rre st

1 2 3 4 7 5 6 Chime r ic r e c e ptor

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SLIDE 11

GAL 1 Chime r ic r e c e ptor GF P te r minator

Induc e d by Galac tose Not induc e d

T =45min T =90min

(a) (b) (c)

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SLIDE 12

GAL 1 Chime r ic r e c e ptor te r minator GAL 1 Rat RI7 r e c e ptor te r minator

1C 2C 1C 2C 1C 2C 1C 2C 4h 2h 0h 4h 2h 0h 4h 2h 0h 4h 2h 0h

He xanal Diac e tyl

(1) (4) (2) (3)

RI7 r e c e ptor Chime r ic r e c e ptor L igand induc tion Contr

  • l

No He xanal No Diac e tyl

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SLIDE 13

α

fa c tor

α

fa c tor

25 μm

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SLIDE 14

Gα

G β

G γ

F AR1 GT P

Oute r Me mbr ane Inne r

MAPK pa thwa y

Gα

G β

G γ

GDP

F US1 Ce ll c yc le a rre st Ma ting

F AR1 GPA1

Gαolf Gαolf

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SLIDE 15
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SLIDE 16

2-phenylethanol

  • Attra c ts dro so phila
  • Vo la tile
  • Pro duc e d in ye a st e ndo g e no us

pa thwa y

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SLIDE 17

Ar

  • 9p

YDR380W YDL 168W 2- phe nylalanine phe nylpyr uvate phe nylac e talde hyde 2- phe nyle thanol

ARO9

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SLIDE 18

α factor

ARO9 ARO9

α factor

GF P

2-phenylethanol

MAPK MAPK

Odorant Odorant

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SLIDE 19
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SLIDE 20
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SLIDE 21

BinB GST GAL1 GAL10 GST BinA Terminator Terminator

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SLIDE 22

Co nstruc tio n o f the RI

7-ODR-10 c hime ric re c e pto r

L

  • c a liza tio n a nd func tio na l a ssa y o f the

c hime ric re c e pto r in S. c e re visiae

Co nstruc tio n o f a ΔGPA1 , ΔF

AR1, ΔARO9

  • S. c e re visiae stra in

Co ntrib utio n o f Bio Bric ks to the Re g istry Cha ra c te riza tio n o f Bio Bric k Pa rts

(BBa _K 211001;BBa _K 211002)

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SLIDE 23

Optimiza tio n o f the GPCR c o uple d

pa thwa y fo r mo re e ffic ie nt o do ra nt se nsing

Ong o ing c o nstruc tio n o f the a ttra c ta nt

pro duc ing c irc uit a nd c ha ra c te riza tio n

  • f the re po rte r func tio na l a ssa y

Ong o ing c o nstruc tio n o f the to xin

pro duc ing c irc uit a nd b io lo g ic a l te st

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SLIDE 24

L igand binding domain RI7N RI7C

MAPK F US1 pr

  • mote r

F US1 te r minator

Attractant

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SLIDE 25

Curbing the population of pathogen vectors

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SLIDE 26

Model system to screen for GPCRs that bind to certain ligands.

ODR- 10 ligand binding domain RI7N RI7C

MAPK

hormones, odorants, neurotransmitters, medicines…

MAPK GF P

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SLIDE 27

Instr uc tor and Advisor s fr

  • m HKUST

:

  • Pro fe sso r K

ing L . CHOW

  • Gua ng na n T

I AN

  • Mr. Wa i-Pa ng HO

Othe r s:

  • Pro fe sso r Chun L

I ANG a nd Mr. Re ntia n WU, HK UST

  • Pro fe sso r Da vid K

a rl BANF I E L D, HK UST

  • Dr. Je ssic a Ce Mun T

ANG, HK UST

  • Pro fe sso r Stua rt F

I RE ST E I N, Co lumb ia Unive rsity

  • Pro fe sso r Pia li SE

NGUPT A, Bra nde is Unive rsity

  • Dr. Bo o nhia ng PROMDONK

OY, BI OT E C

  • Dr. E

dith PAJOT

  • AUGY, I

NRA- Unive rsité Pa ris-Sud

  • L

e e -Hysa n F

  • unda tio n
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SLIDE 28
  • A Synthe tic Pa thwa y fo r Pe st Co ntro l