OBT Formation in Darkness of Photosynthesis is based one - - PowerPoint PPT Presentation
OBT Formation in Darkness of Photosynthesis is based one re-valuation of ONTARIO HYDRO Report No 84-69-K (1984) by F. S. Spencer Tritiated Water Uptake Kinetics in Tissue- Free Water and Organically Bound Fractions of Tomato Plants. 9 Weeks
ONTARIO HYDRO Report No 84-69-K (1984) by F. S. Spencer Tritiated Water Uptake Kinetics in Tissue- Free Water and Organically Bound Fractions
is based one re-valuation of
9 Weeks HTO exposure ,16 hours illumination, 8 hours darkness
168 336 504 672 840 1008 1176 1344 1512 Hours HTO Exposure 5.000 10.000 15.000 20.000 25.000 30.000 35.000 OBT[pCi/gH] 14d 7d 21d 28d 35d 42d 49d 56d 63d
OBT Data of Tomato Plants During 9 Weeks of HTOExposure
Formation Time Product OBT = B*2t/τ A
Biological formation and mono-molecular kinetics start exponential according to F(t) = A*2**t/τ Biological formation and mono-molecular kinetics start exponential according to F(t) = A*2**t/τ and terminate according to F(t) = A*(1−2−(t−to)/τ) = A − C* 2−t/τ
Both, in biological product formation and in mono-molecular reactions exist a self-accelerating and a self-inhibiting phase
Formation Time OBT = A*(1-2–(t-to)/τ) = A – C*2t/τ A Formation Time OBT = A*(1-2–(t-to)/τ) = A – C*2t/τ OBT = B*2t/τ A
Self- accelerating phase Self- inhibiting phase
168 336 504 672 840 1008 1176 1344 1512
Hours HTO Exposure
5.000 10.000 15.000 20.000 25.000 30.000 35.000 OBT [pCi/gH] 14d 7d 21d 28d 35d 42d 49d 56d 63d 168 336 504 672 840 1008 1176 1344 1512
Hours HTO Exposure
5.000 10.000 15.000 20.000 25.000 30.000 35.000 OBT [pCi/gH] 14d 7d 21d 28d 35d 42d 49d 56d 63d
2 inhibiting phases ! 1 Pathway to CBT by Photosynthesis Late T+/H+ Exch Early T+/H+
The OBT Data of Tomato Plants During 9 Weeks of HTOExposure
2 OBT Pathways to OBT by H+/T+ Exchange
168 336 504 672 840 1008 1176 1344 1512
Hours HTO Exposure
5.000 10.000 15.000 20.000 25.000 30.000 35.000 OBT [pCi/gH] 14d 7d 21d 28d 35d 42d 49d 56d 63d
Late H+/T+ exchange self- accelerating Phase Late H+/T+ exchange self- inhibiting Phase Photosynthesis Early H+/T+ exchange
A look at details of 700 h growth
168 336 504 672 Hours HTO Exposure 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 OBT[pCi/gH] 14d 7d 21d 28d 168 336 504 672 Hours HTO Exposure 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 OBT[pCi/gH] 14d 7d 21d 28d
Photosynthesis inhibiting phase Early H+/T+ Late H+/T+ Photosynthesis accelerating phase by approximation
168 336 504 672 Hours HTO Exposure 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 OBT[pCi/gH] 14d 7d 21d 28d
Dark phases during photosynthesis
A look at details of 24 h growth
24 Hours HTO Exposure 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 OBT[pCi/gH] 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 TFWT [pCi/mL] 24 Hours HTO Exposure 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 OBT[pCi/gH] 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 TFWT [pCi/mL]
1d
24 Hours HTO Exposure 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 OBT[pCi/gH] 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 TFWT [pCi/mL] 24 Hours HTO Exposure 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 OBT[pCi/gH] 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 TFWT [pCi/mL]
1d
Accelerating Photosynthetic Phase Fast T+/H+ Accelerating Photosynthetic Phase There is no OBT formation by photosynthesis at night. But there is OBT formation at night by fast H+/T+ exchange
Steady‐state atmospheric HTO: 95080±11280 pCi/gH Photosynthetic photon flux density: 500→430 mE/s/m2 6 Plants (14‐16 weeks old) Lower = Old Foliage → abrupt exposure Upper = New Foliage → chronic exposure
24 48 72 96 120 144 168 Hours HTO Exposure 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 OBT[pCi/gH] 2d 1d 3d 5d 4d 6d 7d 24 48 72 96 120 144 168 Hours HTO Exposure 1.000 2.000 3.000 4.000 5.000 6.000 7.000 8.000 9.000 10.000 OBT[pCi/gH] 2d 1d 3d 5d 4d 6d 7d
OBT formation during 7 days During accelererating Photosynthesis OBTformation is inferior to fast H+/T+ Early T+/H+
A look at details of 1 day growth
Accelerating Photo-syn- thetic phase