Nature is a great source of inspiration for innovation and - - PowerPoint PPT Presentation

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Oct 25, 2022 29 likes •339 views

S ynthetic U nits for R edirecting F unctionalities Nature is a great source of inspiration for innovation and application Flagella: Conserved function but high variability Allowing more flexibility: SilverSURF B. subtilis S. typhimurium S.

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Synthetic Units for Redirecting Functionalities

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Nature is a great source of inspiration for innovation and application

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Flagella: Conserved function but high variability

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Allowing more flexibility: SilverSURF

B. subtilis
S. typhimurium
S. cerevisiae

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Domain insertion leads to altered flagellum morphology

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Can we use the scaffolding potential of the flagellar filament for other purposes?

DARPin Ec Ec1

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We wanted to investigate the effect of inserting a DARPin into the Flagellin

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We had to think of an alternative…

Streptavidin DARPin Ec1 StrepDARPidin

Improve a brick:

iGEM-Team Harvard 2006

Strep-tagged Filament

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EpCAM

StrepDARPidin binds to EpCAM+ cancer cells

EpCAM+ EpCAM+ EpCAM-

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A binding assay confirms the interaction between Strep-tagged Flagellin and StrepDARPidin

Strep-tagged Flagellin StrepDARPidin

Flagellin StrepDARPidin

St Stre rep-tagged Flagellin & St Stre repDARPidin

StrepDARPidin Strep-tagged Flagellin

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Our system could be used for different medical applications in the future

Induce Apoptosis Fluorescent marking Immunesystem activation

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tRNA binding domain Arc1p-C

Redirecting the whole protein assembly machinery

Grand prize 2013 Heidelberg

Amino acid activating domain PheA Improve a brick:

Heidelberg 2013

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The molecular model enables us to predict well-working fusion proteins

Special thanks to our advisor Florian Altegoer for helping us with the crystallization

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To test our model we synthesized the fusion proteins

Match of model and reality

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An example of a possible new application: Marking of proteins

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What is the use of the best application without safety?

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What happens if a mutation occurs?

Constitutive promoter

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The worst case: Let us assume we lose the essential gene.

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Time course of YvyD concentration

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Mutation safe by three layers of security

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The Big Picture

SilverSURF CancerSURF RiboSURF

SURFkiller

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SynAesthetic Biology:

Science without Sight

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True equals visualisable?

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Cooperation

the mission: capacity building

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Preparation

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BLISTA

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How do you show someone who can not see, the things that you can not see yourself? (and where you rely on visualization techniques)

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Do we really know what we see – or do we see what we know?

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Attributions

AG Marahiel AG Bange AG Bölker AG Maierl AG Steinhoff AG Graumann AG Voigt AG Waldminghaus AG Grosse AG Hoyer

Dr. Gundula Meißner, Bettina Happel, Prof. Dr. Christian Wagner,
Dr. V. Krey (TUMunich), Alex Schmidt and Markus Esswein

BLISTA Marburg Instructors:

Prof. Dr. Michael Bölker and Dr. Gert Bange

Advisor:

Dr. Georg Fritz, Daniel Schindler, Felix Dempwolf and Florian Altegoer

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