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Nov 25, 2023 •48 likes •508 views

Nagahama About our Institute Nagahama Institute of Bio-Science and Technology Japan, Shiga, Nagahama city rice How to solve Plasmid construction-1 RBS MT Cd p Catching E.coli has two merits Cd Cd Cd Catching E. coli is

Nagahama
About our Institute • Nagahama Institute of Bio-Science and Technology Japan, Shiga, Nagahama city
rice
How to solve
Plasmid construction-1 RBS MT Cd p
Catching E.coli has two merits
Cd² ⁺ Cd² ⁺ Cd² ⁺ Catching E. coli is not weaken or is killed by absorbed cadmium. Cd² ⁺ Cd² ⁺ Cd² ⁺ Cd² ⁺
Recyclable system of cadmium Cd² ⁺ Cd² ⁺ Cd² ⁺ Cd² ⁺ Cd² ⁺ Cd² ⁺
We have constructed fusion protein RBS MT Cd p
But we have problems
E. coli may escape from cadmium by negative chemotaxis.
Thanks for
Modeling by UT-Tokyo
Swarming assay E. coli E. coli E. coli go away from cadmium!!! Cd ddH2O
If use only catching E. coli
How to solve? Gathering to cadmium ion → Positive chemotaxis Positive chemotaxis factor → Aspartic acid(Asp)
Go to Asp?
E. coli Asp_concentration
E. coli go to Asp!!!
Using AspA OM IM fumafic acid aspartic acid ammonium ion RBS AspA Cd p
Aspartic acid synthesis by Gathering E. coli − Cd 2+ + Cd 2+ 1 50 1 200 1 100 1 100 1 50 1
Overview
protease ClpXP Protease: ClpAP Digested!! AspA LVA tag
Remove LVA tag AspA LVA tag BBa_C0083
Policy & Practice
“The 86 th Annual Meeting of The Genetics Society of Japan”
We conducted a presentation Policy&Practice
We did a poster session Policy&Practice
We did a poster session Policy&Practice
We did a poster session Policy&Practice
ＮｉｃｅＳｍｉｌｅ！
Acknowledgement • Instructors Assosiate Prof. Shoji Usami Prof. Yoshisuke Nishi
Acknowledgement ・ Advisors Ryuhei Minei Third degree of undergraduate student
Acknowledgement • Swarming assay : NIB. Plant molecular Physiology laboratory • Fusion protein : Prof. Atsushi Ohshima • Cd handling : Prof. Yasushi Kawai
Acknowledgement Camera : Prof. Sanji Matsushima TLC : Assosiate Prof.Makoto Hasegawa SDS-PAGE: Assistant staff Takashi Hamada
Acknowledgement Laboratory equipment : Assistant staff of Dept. Biosci. Genom ics Course Assistant staff of Dept. Biosci. Protemics Course Assistant staff of Dept. Biosci. Cell Bio Course Assistant staff of Dept. Biosci. Enviro Bio Course Assistant staff of Dept. Animal Biosci
Acknowledgement • Presentation support Assosiate Prof.Kazue Asou Hayato Saigo • Modeling support UT-Tokyo
Sponsors
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