Nagahama About our Institute Nagahama Institute of Bio-Science and - - PowerPoint PPT Presentation

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Nagahama About our Institute Nagahama Institute of Bio-Science and - - PowerPoint PPT Presentation

Nov 25, 2023 48 likes •513 views

Nagahama About our Institute Nagahama Institute of Bio-Science and Technology Japan, Shiga, Nagahama city rice How to solve Plasmid construction-1 RBS MT Cd p Catching E.coli has two merits Cd Cd Cd Catching E. coli is

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Nagahama

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About our Institute

Japan, Shiga, Nagahama city

  • Nagahama Institute of Bio-Science and Technology
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rice

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How to solve

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Plasmid construction-1

MT RBS

Cd p

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Catching E.coli has two merits

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Catching E. coli is not weaken or is killed by absorbed cadmium.

Cd²⁺ Cd²⁺ Cd²⁺ Cd²⁺ Cd²⁺ Cd²⁺ Cd²⁺

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Recyclable system of cadmium

Cd²⁺ Cd²⁺ Cd²⁺ Cd²⁺ Cd²⁺ Cd²⁺

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We have constructed fusion protein

MT RBS

Cd p

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But we have problems

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  • E. coli may escape from cadmium

by negative chemotaxis.

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Thanks for

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Modeling by UT-Tokyo

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Swarming assay

  • E. coli
  • E. coli

Cd ddH2O

  • E. coli go

away from cadmium!!!

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If use only catching E. coli

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How to solve?

Gathering to cadmium ion →Positive chemotaxis Positive chemotaxis factor →Aspartic acid(Asp)

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Go to Asp?

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  • E. coli

Asp_concentration

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  • E. coli go to Asp!!!
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Using AspA

RBS AspA

fumafic acid ammonium ion aspartic acid

Cd p IM OM

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Aspartic acid synthesis by Gathering E. coli

+ Cd2+ − Cd2+

1 50 1 200 1 100 1 50 1 1 100

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Overview

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protease

ClpXP ClpAP Protease:

Digested!!

AspA LVA tag

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Remove LVA tag

AspA LVA tag

BBa_C0083

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Policy & Practice

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“The 86th Annual Meeting of The Genetics Society of Japan”

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Policy&Practice

We conducted a presentation

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Policy&Practice

We did a poster session

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Policy&Practice

We did a poster session

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Policy&Practice

We did a poster session

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Ｎｉｃｅ Ｓｍｉｌｅ！

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Acknowledgement

  • Instructors

Assosiate Prof. Shoji Usami

  • Prof. Yoshisuke Nishi
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Acknowledgement

・Advisors Ryuhei Minei

Third degree of undergraduate student

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Acknowledgement

  • Swarming assay :
  • NIB. Plant molecular Physiology laboratory
  • Fusion protein :
  • Prof. Atsushi Ohshima
  • Cd handling :
  • Prof. Yasushi Kawai
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Acknowledgement

Camera :

  • Prof. Sanji Matsushima

TLC : Assosiate Prof.Makoto Hasegawa SDS-PAGE: Assistant staff Takashi Hamada

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Acknowledgement

Laboratory equipment : Assistant staff of Dept. Biosci. Genom ics Course Assistant staff of Dept. Biosci. Protemics Course Assistant staff of Dept. Biosci. Cell Bio Course Assistant staff of Dept. Biosci. Enviro Bio Course Assistant staff of Dept. Animal Biosci

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Acknowledgement

  • Presentation support

Assosiate Prof.Kazue Asou Hayato Saigo

  • Modeling support

UT-Tokyo

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Sponsors

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Thank you for listening of our presentation

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