SLIDE 1
2
Top down approach Bottom up approach
The two main approach for the protein identification including
J Proteomic res 2002, 1: 239-52
Endoproteinase and cleaving agents
C-Term E > D High E V8 endopeptidase C-Term S, T Medium C S-ethyl trifluorothioacetate N-Term D and cysteic acid… high E Peptidyl Asp N C-Term R > K High E Trypsin C-Term D Medium C Pentafluoropropionic acid C-Term P>>> medium E Prolyl endopeptidase (recombinante) C-Term P >> A, S Low E Prolyl endopeptidase C-Term and N- Term F, Y, L > E, C Very low E Pepsin C-Term W >> Y High C
- -Iodosobenzoic acid
C-Term W > Y, H medium C N-Halogenosuccinimid C-Term K Very high E Lysyl endopeptidase C-Term M Very high C Cyanogen bromide C-Term R >> K High E Clostripain C-Term W> Y> F>> L >M > H Low E Chymotrypsin C-Term depending to the conditions Recurrent hydrolysis Low C Acidic hydrolysis At the Cleaved residue(s) Spécificity Enzyme or Chemical Cleaving agent