microRNA33 targets the ABCA1 pump and regulates cholesterol - PowerPoint PPT Presentation

Role of miR-33 in lipid metabolism Laure-Alix Clerbaux Université catholique de Louvain Faculté de Médecine Laboratoire de chimie physiologique de Duve Institute microRNA33 targets the ABCA1 pump and regulates cholesterol metabolism Laure-Alix Clerbaux Promoter : Guido Bommer Co-promoter : Emile Van Schaftingen

Introduction Role of miR-33 in lipid metabolism microRNA inhibits translation of their target transcript mature miRNA

Introduction Role of miR-33 in lipid metabolism mature miRNA

Results Role of miR-33 in lipid metabolism miR33 is located in an intron of SREBP gene Gerin*, Clerbaux* et al ., 2010 in JBC

Results Role of miR-33 in lipid metabolism This location and miR33a sequence is well conserved � Collaboration between coding-protein gene and miR ? Gerin*, Clerbaux* et al ., 2010 in JBC

Results Role of miR-33 in lipid metabolism SREBP protein plays a role in lipid homeostasis Gerin*, Clerbaux* et al ., 2010 in JBC

Results Role of miR-33 in lipid metabolism Does miR33 target genes implicated in lipid homeostasis ? Gerin*, Clerbaux* et al ., 2010 in JBC

Results Role of miR-33 in lipid metabolism miR33 targets the ABCA1 pump Gerin*, Clerbaux* et al ., 2010 in JBC

Results ABCAI and cholesterol export Transporter ABCAI regulates the cellular cholesterol export Rayner et al., 2010; Najafi-Shoushtari et al., 2010; Horie et al., 2010

Results ABCAI and cholesterol export miR33 targets ABCAI Gerin*, Clerbaux* et al ., 2010 in JBC

Results ABCAI and cholesterol export miR33 targets ABCAI Gerin*, Clerbaux* et al ., 2010 in JBC

Results ABCAI and cholesterol export miR33 targets ABCAI Gerin*, Clerbaux* et al ., 2010 in JBC

Results ABCAI and cholesterol export HepG2 cells overexpressing miR33

Results ABCAI and cholesterol export miR33 targets ABCAI and regulates cholesterol export Gerin*, Clerbaux* et al ., 2010 in JBC

Results ABCAI and cholesterol export miR33 targets ABCAI and regulates cholesterol export Gerin*, Clerbaux* et al ., 2010 in JBC

Results ABCAI and cholesterol export miR33 targets ABCAI and regulates cholesterol export Gerin*, Clerbaux* et al ., 2010 in JBC

Results Role of miR-33 in lipid metabolism miR33 collaborates with SREBP to maintain cholesterol homeostasis Gerin*, Clerbaux* et al ., 2010 in JBC

Results ABCAI and cholesterol export miR33 increases plasma HDL levels QuickTime™ et un décompresseur sont requis pour visionner cette image. Rayner et al., 2010; Najafi-Shoushtari et al., 2010; Horie et al., 2010; Rayner et al., 2011