microRNA33 targets the ABCA1 pump and regulates cholesterol - - PowerPoint PPT Presentation

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Nov 07, 2023 134 likes •325 views

Role of miR-33 in lipid metabolism Laure-Alix Clerbaux Universit catholique de Louvain Facult de Mdecine Laboratoire de chimie physiologique de Duve Institute microRNA33 targets the ABCA1 pump and regulates cholesterol metabolism

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microRNA33 targets the ABCA1 pump and regulates cholesterol metabolism

Laure-Alix Clerbaux Promoter : Guido Bommer Co-promoter : Emile Van Schaftingen

Université catholique de Louvain Faculté de Médecine Laboratoire de chimie physiologique de Duve Institute

Role of miR-33 in lipid metabolism Laure-Alix Clerbaux

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Role of miR-33 in lipid metabolism

Introduction

microRNA inhibits translation of their target transcript

mature miRNA

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Role of miR-33 in lipid metabolism

Introduction

mature miRNA

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miR33 is located in an intron

f SREBP gene

Role of miR-33 in lipid metabolism

Results

Gerin*, Clerbaux* et al., 2010 in JBC

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This location and miR33a sequence is well conserved

Role of miR-33 in lipid metabolism

Results

Gerin*, Clerbaux* et al., 2010 in JBC

Collaboration between coding-protein gene and miR ?

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SREBP protein plays a role in lipid homeostasis

Role of miR-33 in lipid metabolism

Results

Gerin*, Clerbaux* et al., 2010 in JBC

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Does miR33 target genes implicated in lipid homeostasis ?

Role of miR-33 in lipid metabolism

Results

Gerin*, Clerbaux* et al., 2010 in JBC

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miR33 targets the ABCA1 pump

Role of miR-33 in lipid metabolism

Results

Gerin*, Clerbaux* et al., 2010 in JBC

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Transporter ABCAI regulates the cellular cholesterol export

Rayner et al., 2010; Najafi-Shoushtari et al., 2010; Horie et al., 2010

ABCAI and cholesterol export

Results

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miR33 targets ABCAI

ABCAI and cholesterol export

Results

Gerin*, Clerbaux* et al., 2010 in JBC

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miR33 targets ABCAI

ABCAI and cholesterol export

Results

Gerin*, Clerbaux* et al., 2010 in JBC

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miR33 targets ABCAI

ABCAI and cholesterol export

Results

Gerin*, Clerbaux* et al., 2010 in JBC

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HepG2 cells overexpressing miR33

ABCAI and cholesterol export

Results

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miR33 targets ABCAI and regulates cholesterol export

ABCAI and cholesterol export

Results

Gerin*, Clerbaux* et al., 2010 in JBC

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miR33 targets ABCAI and regulates cholesterol export

ABCAI and cholesterol export

Results

Gerin*, Clerbaux* et al., 2010 in JBC

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miR33 targets ABCAI and regulates cholesterol export

Gerin*, Clerbaux* et al., 2010 in JBC

ABCAI and cholesterol export

Results

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miR33 collaborates with SREBP to maintain cholesterol homeostasis

Gerin*, Clerbaux* et al., 2010 in JBC

Role of miR-33 in lipid metabolism

Results

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miR33 increases plasma HDL levels

Rayner et al., 2010; Najafi-Shoushtari et al., 2010; Horie et al., 2010; Rayner et al., 2011

ABCAI and cholesterol export

Results

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