L.) Lettuce is an important agricultural commodity worldwide and it - - PDF document

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L.) Lettuce is an important agricultural commodity worldwide and it - - PDF document

Oral Presentation CRDC 5 21/07/54 EFFECTS OF TYPES AND CONCENTRATIONS OF CHITOSAN ON GROWTH OF FRILLICE ICEBERG LETTUCE CULTIVATED ICEBERG LETTUCE CULTIVATED BY HYDROPONIC METHOD Presented by Tanet Jirapornprasert L ETTUCE ( L


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Oral Presentation CRDC 5 21/07/54 1

EFFECTS OF TYPES AND CONCENTRATIONS OF CHITOSAN ON GROWTH OF ‘FRILLICE ICEBERG’ LETTUCE CULTIVATED ICEBERG’ LETTUCE CULTIVATED BY HYDROPONIC METHOD

Presented by Tanet Jirapornprasert

LETTUCE (LACTUCA SATIVA L.)

Lettuce is an important agricultural commodity

worldwide and it is the most popular vegetable in worldwide and it is the most popular vegetable in salads.

With the increase in the consumption of salads,

lettuce can provide a significant proportion of the total antioxidants in the human diet.

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Oral Presentation CRDC 5 21/07/54 2

CHITOSAN

Deacetylation

  • Conc. HCl
  • Conc. NaOH

CHITOSAN

biopolymer prepared by deacetylation reaction of chitin found in the shells of crustaceans, such as lobsters,

crabs, and shrimp, and many other organisms including insects and fungi

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APPLICATION

Nge et al., 2006

APPLICATION

  • PLB production in medium

without chitosan, or with the addition of P-70 (chitosan 1) at 10 and 80 mg/L, and P-90 (chitosan 2) at 20 and 80 mg/L.

  • Photographed cultures are

representative of at least four independent replications, and they were taken after three

Pornpienpakdee et al.,2010

they were taken after three months in culture.

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OBJECTIVE

Investigate the appropriate use of chitosan to

enhance growth of ‘Frillice Iceberg’ lettuce enhance growth of Frillice Iceberg lettuce (Lactuca sativa L.) cultivate with hydroponic method

MATERIALS AND METHODS

Grown the lettuce for 10 days Transplant to nutrient solution for 35 days and Transplant to nutrient solution for 35 days and

change every 7 days

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7 TREATMENTS

O 80 5 mg/L

  • Chitosan

O O P P 80 80 80 80 g/ 10 mg/L 20 mg/L 5 mg/L 10 mg/L

  • Chitosan
  • Chitosan
  • Chitosan
  • Chitosan

P P 80 80 10 mg/L 20 mg/L

  • Chitosan
  • Chitosan
  • Control: Without chitosan

Fresh weight

Store at 8°C for 10 days

Fresh weight after storage Dry weight

Store at 60°C for 3 days

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RESULTS RESULTS

AND

DISCUSSION DISCUSSION

FRESH WEIGHT

50

a a ab ab

10 20 30 40

Fresh weight(g) b b ab ab ab

control O 5 O 10 O 20 P 5 P 10 P 20

O5 = 5 mg/L oligomeric chitosan O10 = 10 mg/L oligomeric chitosan O20 = 20 mg/L oligomeric chitosan P5 = 5 mg/L polymeric chitosan P10 = 10 mg/L polymeric chitosan P20 = 20 mg/L polymeric chitosan

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DRY WEIGHT

2.5 0.5 1.0 1.5 2.0

Dry weight(g)

0.0 control O 5 O 10 O 20 P 5 P 10 P 20

O5 = 5 mg/L oligomeric chitosan O10 = 10 mg/L oligomeric chitosan O20 = 20 mg/L oligomeric chitosan P5 = 5 mg/L polymeric chitosan P10 = 10 mg/L polymeric chitosan P20 = 20 mg/L polymeric chitosan

WEIGHT LOSS PERCENTAGE

6 7 1 2 3 4 5

Weight loss percentage

control O 5 O 10 O 20 P 5 P 10 P 20

O5 = 5 mg/L oligomeric chitosan O10 = 10 mg/L oligomeric chitosan O20 = 20 mg/L oligomeric chitosan P5 = 5 mg/L polymeric chitosan P10 = 10 mg/L polymeric chitosan P20 = 20 mg/L polymeric chitosan

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7 TREATMENTS

O 80 0.1 mg/L

  • Chitosan

O O P P 80 80 80 80 g/ 1 mg/L 5 mg/L 0.1 mg/L 1 mg/L

  • Chitosan
  • Chitosan
  • Chitosan
  • Chitosan

P P 80 80 1 mg/L 5 mg/L

  • Chitosan
  • Chitosan
  • Control: Without chitosan

FRESH WEIGHT

120

ab ab a

20 40 60 80 100

Fresh weight(g) b ab b b b O0.1 = 0.1 mg/L oligomeric chitosan O1 = 1 mg/L oligomeric chitosan O5 = 5 mg/L oligomeric chitosan P0.1 = 0.1 mg/L polymeric chitosan P1 = 1 mg/L polymeric chitosan P5 = 5 mg/L polymeric chitosan

control O5 O1 O0.1 P5 P1 P0.1

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DRY WEIGHT

3.0 0.5 1.0 1.5 2.0 2.5

Dry weight(g)

0.0 control O5 O1 O0.1 P5 P1 P0.1

O0.1 = 0.1 mg/L oligomeric chitosan O1 = 1 mg/L oligomeric chitosan O5 = 5 mg/L oligomeric chitosan P0.1 = 0.1 mg/L polymeric chitosan P1 = 1 mg/L polymeric chitosan P5 = 5 mg/L polymeric chitosan

WEIGHT LOSS PERCENTAGE

5 1 2 3 4

Weight loss percentage

control O5 O1 O0.1 P5 P1 P0.1

O0.1 = 0.1 mg/L oligomeric chitosan O1 = 1 mg/L oligomeric chitosan O5 = 5 mg/L oligomeric chitosan P0.1 = 0.1 mg/L polymeric chitosan P1 = 1 mg/L polymeric chitosan P5 = 5 mg/L polymeric chitosan

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Nge et al 2006 Nge et al., 2006

Chitosan concentration (mg/L) Fresh weight (g) after three months Control

3.02 ± 0.33abcdef 10 4 08 ± 1 26abc

Pornpienpakdee et al.,2010

P-80

10 4.08 ± 1.26abc 20 4.03 ± 1.24abc 40 3.29 ± 1.43abcde 80 0.73 ± 0.16f

O-80

10 2.83 ± 0.97bcdef 20 3.51 ± 0.74abcd 40 1.89 ± 0.30cdef 80 0.77 ± 0.22f

  • Effect of chitosan on light-induced

stomatal opening in tomato leaf epidermis. Note that 10 mM ascorbic acid suppressed the inhibitory effect of chitosan (200 g/mL ) on stomatal

  • pening
  • Results are the averages ± SE

(n = 120) of three to four independent experiments. Lee et al.,1999

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CONCLUSION

1 mg/L oligomeric chitosan with 80 degree of

deacetylation is the appropriate use of chitosan to deacetylation is the appropriate use of chitosan to enhance growth of ‘Frillice Iceberg’ lettuce (Lactuca sativa L.) cultivate with hydroponic method ACKNOWLEDGEMENT

Environment and Plant Physiology Research Environment and Plant Physiology Research

Unit, Department of Botany, Faculty of Science, Chulalongkorn University.

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THANK YOU

FOR YOUR ATTENTIONS