Oral Presentation CRDC 5 21/07/54 EFFECTS OF TYPES AND CONCENTRATIONS OF CHITOSAN ON GROWTH OF ‘FRILLICE ICEBERG’ LETTUCE CULTIVATED ICEBERG’ LETTUCE CULTIVATED BY HYDROPONIC METHOD Presented by Tanet Jirapornprasert L ETTUCE ( L ACTUCA SATIVA L.) � Lettuce is an important agricultural commodity worldwide and it is the most popular vegetable in worldwide and it is the most popular vegetable in salads. � With the increase in the consumption of salads, lettuce can provide a significant proportion of the total antioxidants in the human diet. 1
Oral Presentation CRDC 5 21/07/54 C HITOSAN Deacetylation Conc. HCl Conc. NaOH C HITOSAN � biopolymer � prepared by deacetylation reaction of chitin � found in the shells of crustaceans, such as lobsters, crabs, and shrimp, and many other organisms including insects and fungi 2
Oral Presentation CRDC 5 21/07/54 A PPLICATION Nge et al ., 2006 A PPLICATION •PLB production in medium without chitosan, or with the addition of P-70 (chitosan 1) at 10 and 80 mg/L, and P-90 (chitosan 2) at 20 and 80 mg/L. • Photographed cultures are representative of at least four independent replications, and they were taken after three they were taken after three months in culture. Pornpienpakdee et al., 2010 3
Oral Presentation CRDC 5 21/07/54 O BJECTIVE � Investigate the appropriate use of chitosan to enhance growth of ‘Frillice Iceberg’ lettuce enhance growth of Frillice Iceberg lettuce ( Lactuca sativa L.) cultivate with hydroponic method M ATERIALS AND M ETHODS � Grown the lettuce for 10 days � Transplant to nutrient solution for 35 days and � Transplant to nutrient solution for 35 days and change every 7 days 4
Oral Presentation CRDC 5 21/07/54 7 TREATMENTS Chitosan O 80 5 mg/L g/ • Chitosan O 80 10 mg/L • Chitosan O 80 20 mg/L • Chitosan P 80 5 mg/L • Chitosan Chitosan P P 80 80 10 mg/L 10 mg/L • • Chitosan P 80 20 mg/L • Control: Without chitosan • Fresh weight Store at 8°C for 10 days Fresh weight after storage Store at 60°C for 3 days Dry weight 5
Oral Presentation CRDC 5 21/07/54 R ESULTS R ESULTS AND D ISCUSSION D ISCUSSION F RESH WEIGHT 50 a a ab ab ab ab 40 ab b b Fresh weight(g) 30 20 10 0 control O 5 O 10 O 20 P 5 P 10 P 20 O5 = 5 mg/L oligomeric chitosan O10 = 10 mg/L oligomeric chitosan O20 = 20 mg/L oligomeric chitosan P5 = 5 mg/L polymeric chitosan P10 = 10 mg/L polymeric chitosan P20 = 20 mg/L polymeric chitosan 6
Oral Presentation CRDC 5 21/07/54 D RY WEIGHT 2.5 2.0 Dry weight(g) 1.5 1.0 0.5 0.0 control O 5 O 10 O 20 P 5 P 10 P 20 O5 = 5 mg/L oligomeric chitosan O10 = 10 mg/L oligomeric chitosan O20 = 20 mg/L oligomeric chitosan P5 = 5 mg/L polymeric chitosan P10 = 10 mg/L polymeric chitosan P20 = 20 mg/L polymeric chitosan W EIGHT LOSS PERCENTAGE 7 6 Weight loss percentage 5 4 3 2 1 0 control O 5 O 10 O 20 P 5 P 10 P 20 O5 = 5 mg/L oligomeric chitosan O10 = 10 mg/L oligomeric chitosan O20 = 20 mg/L oligomeric chitosan P5 = 5 mg/L polymeric chitosan P10 = 10 mg/L polymeric chitosan P20 = 20 mg/L polymeric chitosan 7
Oral Presentation CRDC 5 21/07/54 7 TREATMENTS Chitosan O 80 0.1 mg/L g/ • Chitosan O 80 1 mg/L • Chitosan O 80 5 mg/L • Chitosan P 80 0.1 mg/L • Chitosan Chitosan P P 80 80 1 mg/L 1 mg/L • • Chitosan P 80 5 mg/L • Control: Without chitosan • F RESH WEIGHT 120 a ab ab ab 100 b b b b Fresh weight(g) 80 60 40 20 0 control O5 O1 O0.1 P5 P1 P0.1 O0.1 = 0.1 mg/L oligomeric chitosan O1 = 1 mg/L oligomeric chitosan O5 = 5 mg/L oligomeric chitosan P0.1 = 0.1 mg/L polymeric chitosan P1 = 1 mg/L polymeric chitosan P5 = 5 mg/L polymeric chitosan 8
Oral Presentation CRDC 5 21/07/54 D RY WEIGHT 3.0 2.5 2.0 Dry weight(g) 1.5 1.0 0.5 0.0 control O5 O1 O0.1 P5 P1 P0.1 O0.1 = 0.1 mg/L oligomeric chitosan O1 = 1 mg/L oligomeric chitosan O5 = 5 mg/L oligomeric chitosan P0.1 = 0.1 mg/L polymeric chitosan P1 = 1 mg/L polymeric chitosan P5 = 5 mg/L polymeric chitosan W EIGHT LOSS PERCENTAGE 5 4 Weight loss percentage 3 2 1 0 control O5 O1 O0.1 P5 P1 P0.1 O0.1 = 0.1 mg/L oligomeric chitosan O1 = 1 mg/L oligomeric chitosan O5 = 5 mg/L oligomeric chitosan P0.1 = 0.1 mg/L polymeric chitosan P1 = 1 mg/L polymeric chitosan P5 = 5 mg/L polymeric chitosan 9
Oral Presentation CRDC 5 21/07/54 Nge et al Nge et al ., 2006 2006 Chitosan concentration Fresh weight (g) after three (mg/L) months 0 3.02 ± 0.33 abcdef Control 10 10 4 08 ± 1 26 abc 4.08 ± 1.26 abc 20 4.03 ± 1.24 abc Pornpienpakdee et al., 2010 P-80 40 3.29 ± 1.43 abcde 80 0.73 ± 0.16 f 10 2.83 ± 0.97 bcdef 20 3.51 ± 0.74 abcd O-80 40 1.89 ± 0.30 cdef 80 0.77 ± 0.22 f •Effect of chitosan on light-induced stomatal opening in tomato leaf epidermis. Note that 10 mM ascorbic acid suppressed the inhibitory effect of chitosan (200 g/mL ) on stomatal opening •Results are the averages ± SE (n = 120) of three to four independent experiments. Lee et al .,1999 10
Oral Presentation CRDC 5 21/07/54 C ONCLUSION � 1 mg/L oligomeric chitosan with 80 degree of deacetylation is the appropriate use of chitosan to deacetylation is the appropriate use of chitosan to enhance growth of ‘Frillice Iceberg’ lettuce ( Lactuca sativa L.) cultivate with hydroponic method ACKNOWLEDGEMENT � Environment and Plant Physiology Research � Environment and Plant Physiology Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University. 11
Oral Presentation CRDC 5 21/07/54 T HANK YOU FOR YOUR ATTENTIONS 12
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