Kinetics of Nanoparticles delivery to pancreatic cancer cells - PowerPoint PPT Presentation

Kinetics of Nanoparticles delivery to pancreatic cancer cells NICOLE HOFFMANN

Introduction Nanomaterial ◦ 1 to 100 nanometers Nanoparticles (NPs) ◦ One type Drug delivery ◦ Small ◦ Easily diffuse through the cell ◦ Antibodies

Overview The NPs have great potential for biomedical applications ◦ Size ◦ Fluorescent dye How do we make the particles better? How do we effectively utilize them? Worked with Dr. Korampally and Dr. Elsawa

NP Background Clump together over time ◦ Need to optimize stability Hydrophobic core ◦ Encases dye Hydrophilic shell ◦ Water soluble Traceable in Cells ◦ Fluorescent

Pancreatic Cancer Model ◦ Shows benefits of NPs Panc-1 ◦ Adherent ◦ Previous results

Methods Create cores using PMSSQ, rhodamine chloride dye, and PPG Age 25 days Create shells using ammonium hydroxide Age 25 days Add hydrochloric acid to remove charge Particle vials before recovery

Methods Cont. NPs imaged with flash photography Recovered NPs in DI

Methods Cont. Centrifuge NPs after being centrifuged during the recovery process Add ammonium hydroxide to return charge Grow 24 wells of Panc-1 cancer cells

Methods Cont. Pass cells to continue growth Add different concentrations of NPs to the wells

Methods Cont. Run timed additions of NPs

Analysis Concentration and fluorescence

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Analysis Cont. 5uL

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Analysis Cont. 40uL

Analysis Cont. Time and fluorescence

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Analysis Cont. Ctrl 5 min. 30 min. 1 hr. 2 hrs. 4 hrs. 6 hrs.

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Engineering Results o 80 mg of dye is effectively encased in the particles o Different concentrations of dye are being tested to find the optimal amount o Low concentrations of dye have already proven unsuccessful and quickly coagulate o NPs created remain evenly dispersed throughout the solution

Biological Results o Confirmed the hypothesis that increased quantities of NPs increases the fluorescence o Work will be done to pinpoint the time necessary for NP absorption o Decrease the amount of time wasted o Optimize productivity and increase quantity of experiments

Discussion o Couple NPs with something toxic to pancreatic cancer cells as a possible cancer treatment o See if attaching different compounds to the NPs enhances the delivery o Compute NP retention in cells o Calculate number of dyes per particle o Analyze lifetime of NPs at Argonne National Laboratory

Acknowledgments McKearn Fellows Program OSEEL NIU Everyone at Dr. Elsawa and Dr. Korampally’s labs