Kinetics of Nanoparticles delivery to pancreatic cancer cells - - PowerPoint PPT Presentation

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Kinetics of Nanoparticles delivery to pancreatic cancer cells - - PowerPoint PPT Presentation

Dec 03, 2023 29 likes •371 views

Kinetics of Nanoparticles delivery to pancreatic cancer cells NICOLE HOFFMANN Introduction Nanomaterial 1 to 100 nanometers Nanoparticles (NPs) One type Drug delivery Small Easily diffuse through the cell Antibodies

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SLIDE 1

Kinetics of Nanoparticles delivery to pancreatic cancer cells

NICOLE HOFFMANN

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SLIDE 2

Introduction

Nanomaterial

  • 1 to 100 nanometers

Nanoparticles (NPs)

  • One type

Drug delivery

  • Small
  • Easily diffuse through the cell
  • Antibodies
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SLIDE 3

Overview

The NPs have great potential for biomedical applications

  • Size
  • Fluorescent dye

How do we make the particles better? How do we effectively utilize them? Worked with Dr. Korampally and Dr. Elsawa

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SLIDE 4

NP Background

Clump together over time

  • Need to optimize stability

Hydrophobic core

  • Encases dye

Hydrophilic shell

  • Water soluble

Traceable in Cells

  • Fluorescent
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SLIDE 5

Pancreatic Cancer

Model

  • Shows benefits of NPs

Panc-1

  • Adherent
  • Previous results
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SLIDE 6

Methods

Create cores using PMSSQ, rhodamine chloride dye, and PPG Age 25 days Create shells using ammonium hydroxide Age 25 days Add hydrochloric acid to remove charge

Particle vials before recovery

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SLIDE 7

Methods Cont.

NPs imaged with flash photography Recovered NPs in DI

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SLIDE 8

Methods Cont.

Centrifuge Add ammonium hydroxide to return charge Grow 24 wells of Panc-1 cancer cells

NPs after being centrifuged during the recovery process

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SLIDE 9

Methods Cont.

Pass cells to continue growth Add different concentrations of NPs to the wells

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SLIDE 10

Methods Cont.

Run timed additions of NPs

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SLIDE 11

Analysis

Concentration and fluorescence

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SLIDE 12

Analysis Cont.

Ctrl

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SLIDE 13

Analysis Cont.

5uL

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SLIDE 14

Analysis Cont.

10uL

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SLIDE 15

Analysis Cont.

20uL

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SLIDE 16

Analysis Cont.

40uL

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SLIDE 17

Analysis Cont.

Time and fluorescence

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SLIDE 18

Analysis Cont.

Control

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SLIDE 19

Analysis Cont.

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SLIDE 20

Analysis Cont.

30 min.

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SLIDE 21

Analysis Cont.

1 hr.

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SLIDE 22

Analysis Cont.

2 hrs.

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SLIDE 23

Analysis Cont.

4 hrs.

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SLIDE 24

Analysis Cont.

6 hrs.

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SLIDE 25

Analysis Cont.

Ctrl 5 min. 30 min. 1 hr. 2 hrs. 4 hrs. 6 hrs.

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SLIDE 26

Analysis Cont.

Ctrl

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SLIDE 27

Analysis Cont.

0 hrs

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SLIDE 28

Analysis Cont.

2 hrs

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SLIDE 29

Analysis Cont.

4 hrs

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SLIDE 30

Analysis Cont.

6 hrs

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SLIDE 31

Engineering Results

  • 80 mg of dye is effectively encased in the particles
  • Different concentrations of dye are being tested to find the optimal

amount

  • Low concentrations of dye have already proven unsuccessful and

quickly coagulate

  • NPs created remain evenly dispersed throughout the solution
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SLIDE 32

Biological Results

  • Confirmed the hypothesis that increased quantities of NPs increases

the fluorescence

  • Work will be done to pinpoint the time necessary for NP absorption
  • Decrease the amount of time wasted
  • Optimize productivity and increase quantity of experiments
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SLIDE 33

Discussion

  • Couple NPs with something toxic to pancreatic cancer cells as a

possible cancer treatment

  • See if attaching different compounds to the NPs enhances the delivery
  • Compute NP retention in cells
  • Calculate number of dyes per particle
  • Analyze lifetime of NPs at Argonne National Laboratory
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SLIDE 34

Acknowledgments

McKearn Fellows Program OSEEL NIU Everyone at Dr. Elsawa and Dr. Korampally’s labs