Isobionics Company profile Founded in 2008 Our products Flavour - - PowerPoint PPT Presentation

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• Founded in 2008
• Our products – Flavour & Fragrance isoprenoids:

Valencene / Nootkatone / Beta‐Bisabolene

• F&F product pipeline: Citrus terpenoids, sandalwood,

patchouli, C10 – C20 terpenes

• R&D laboratories for strain engineering, fermentation,

downstream processing, and analytics

• Strong scale‐up and production capabilities

Isobionics ‐ Company profile

Valencene Nootkatone

With Valencene, Isobionics was the first company bringing a fermentation‐based terpene flavour to the market

Beta-Bisabolene

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Our products: isoprenoids

Built from isoprene (C5) units Largest group of natural hydrocarbons >50.000 identified compounds Low natural abundance Isobionics technology: scalable, cost‐effective production via fermentation Isoprenoid ingredients markets:

• Food: Vitamins (A,E), Sweeteners (Steviol)
• Pharmaceuticals (Taxol)
• Chemical building blocks (Isoprene)
• Flavors & Fragrances

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We have developed the technology to allow production of isoprenoids via fermentation

F&F isoprenoids

• Monoterpenes (C10)
• Sesquiterpenes (C15)
• Diterpenes (C20)

Isoprenoids occur naturally in plant essential oils Obtained via:

• Plant material extraction

(often as side products)

• Chemical synthesis (only for

simple isoprenoids)

• Fermentation

C10 – Monoterpenes Ketones e.g. Nootkatone Alcohols e.g. Santalol C20 – Diterpenes C15 – Sesquiterpenes e.g. Valencene, Beta‐Bisabolene

Isoprenoids

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• Constant quality & availability
• Not depending on harvest
• Scalable
• Cost effective supply
• Sustainable & renewable resources
• No pesticides

Fermentative production process

Fermentation Distillation

Glucose

Microorganism with terpene synthase

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Traditional valencene production from orange oil:

• Inefficient: 160.000 Oranges for 1 kg Valencene
• Harvest dependent: Taste – Volume – Price
• High environmental footprint – agricultural footprint / pesticide use
• Shortages due to Citrus greening disease

High efficiency – low carbon footprint

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Microbial platform: Rhodobacter

Rhodobacter sphaeroides

• Natural isoprenoid producer: Carotenoids
• High product tolerance
• Scalable and robust
• No problematic off‐odors

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Additional heterologous pathway increases FPP pool

Rhodobacter terpenoid producer platform

AcCoA

mvaA act hcs mvk pmk mvd

MVA IPP

idi IspA

FPP

DMAPP

glucose

G-3P Pyr DXP Terpene TCA

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• Plug‐in terpene synthases: produce specific

terpene or terpene mixture with high yields

• Access to unique and highly active synthases
• Carbon flux directed to terpenes by tuning

down competing pathways

Plug‐in terpene synthases

FPP

Isoprenoid containing Products

Terpene product

}

Heterologous mevalonate pathway genes

Terpene synthase

Sugar

• R. sphaeroides MEP

pathway

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• Own bioprocess development lab
• Improvement of product titer, rate and yield
• In‐house up‐scaling up to 200 l scale
• First industrial scale up in 2010
• Specific DSP Solutions

Bioprocess development & Scale‐up

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High quality production

• Reliable Scale‐up & robust performance at commercial scale
• Established production partnerships
• Sufficient fermentation capacity for growth
• Established quality systems – ISO 22000 certified
• Kosher + Halal approval

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Products & Product Pipeline

Valencene Nootkatone Beta-Bisabolene Citrus ingredients Patchouli Sandalwood

• Natural aroma compound in oranges
• Flavouring agent in beverages, use level ca. 1 ppm
• Also used for the production of nootkatone

Products Product Pipeline

• Grapefruit aroma
• High value flavour with low natural abundance
• Odor threshold < 1ppm, also used in fragrance
• Lemon & bergamot aroma component
• Pleasant balsamic, green odour
• 90% pure, pesticide‐free

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R&D partnerships & Grants

Strictly Confidential – subject to confidentiality provisions Distribution and copying not allowed

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THANK YOU FOR YOUR KIND ATTENTION !

INNOVATIVE CREATORS OF NATURAL INGREDIENTS