INTRODUCTION The 16 th Asian-Australasian Association of Animal - - PDF document

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Effect of Supplementation of Fulvic Acid on the Characteristic of in vitro Ruminal Fermentation

Idat G. Permana, Heri A. Sukria and Dea J. Nurjanah

Department of Animal Nutrition and Feed Technology Faculty of Animal Science – Bogor Agricultural University

The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress Yogyakarta, 11-14 November 2014.

The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress

INTRODUCTION

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The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress

Fulvic acid as supplement influence:

• enzymes secretion
• hormones structure
• absorb heavy metals
• absorb toxic pollutants

Introduction

The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress

Supplementation of Humic and Fulvic Acids in Animal Feed:

• Humic acids improved broiler performance; and

improved perfomance and carcass quality in Pig.

• Fulvic acid as supplement improved

performance and immune system of broiler chickens, so that can produce good quality chicken, safe and healthy.

• Humic acid and fulvic acid in beef cattle

incresead growth rate and feed efficiency (Cusack 2008).

• Humic and fulvic acid in dairy cattle increased

milk fat and decreased mastitis casses.

Introduction

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• The purpose of this study was to determine the

effect of fulvic acid supplementation in ruminant ration on ruminal fermentation characteristic and protozoa population.

Objectives

The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress

MATERIALS & METHODS

• This research was conducted using in vitro technique (Tilley

and Terry, 1963).

• Experiment rations:
• Ration A (TDN 69.4% and CP 16.2%)
• Ration B (TDN 64.7% and CP 15.6%)
• The ration were formulated from nappier grass, rice bran,

corn, pollard, coconut meal, soybean meal and CaCO3

• The ration were suppelemented with fulvic acids. Level of

Fulvic Acid: 0, 2.5, 5.0, 7.5 and 10%.

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• Parameter measured:
• rumen fluid pH, in vitro dry matter digestibility (IVDMD), in vitro
• rganic matter digestibility (IVOMD), total VFAs and NH3

concentrations and protozoa population.

• In vitro Method:
• rumen fluid were collected from local beef cattles.
• experiment rations were incubated in rumen fluid and

McDougall in anaerobic condition at temperature of 39oC for 24 h, followed 24 h in pepsin-HCl solution.

• total VFA was analysed using steam destilation method, while

NH3 concentration was analyzed using Conway’s microdiffusion method.

• rumen protozoa population was counted under microscope

after marked by TBFS soultion

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• Statistical Analysis
• The data obtained from the study were analyzed

using the analysis of variance (ANOVA) and Duncan’s multiple range test.

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Nutrients Ration A Ration B Rumput Gajah 40,0 50,0 Dedak Halus 6,0 8,0 Jagung 20,3 14,3 Pollard 5,8 5,0 Bungkil Kelapa 24,0 18,0 Bungkil Kedelai 2,0 2,9 CaCO3 1,90 1,8 Ca (%) 2.0 2.8 CaCO3 1,9 1,8

Table 1. Feed ingredient composition

The 16th Asian-Australasian Association of Animal Production Societies (AAAP) Congress

Nutrients Ration A Ration B2 Dry matter 86.88 86.76 Ash 10.66 13.14 Crude protein 16.24 15.64 Crude lipid 3.25 3.47 Crude fibre 21.05 24.42 Nitrogen free extract 52.68 46.99 TDN 69.40 64.66 Ca (%) 2.04 2.81 P (%) 0.46 0.73

Table 2. Nutrient composition of experimental rations

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Level of Fulvic Acid Rumen pH Ration A Ration B Control Ration (R0) 6.96 ± 0.09c 6.95 ± 0.08b R0 + 2.5% fulvic acid 7.02 ± 0.11bc 7.02 ± 0.12a R0 + 5.0% fulvic acid 7.10 ± 0.12a 7.07 ± 0.13a R0 + 7.5% fulvic acid 7.03 ± 0.11b 7.07 ± 0.11a R0 + 10% fulvic acid 7.06 ± 0.13ab 7.08 ± 0.10a Average 7.03 ± 0.02 7.04 ± 0.02

RESULTS AND DISCUSION

Table 3. Effect of fulvic acid supplementation

• n rumen pH

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Level of Fulvic Acid IVDMD (%) Ration A Ration B Control Ration (R0) 65.81 ± 0.91 64.81 ± 5.88 R0 + 2.5% fulvic acid 66.92 ± 3.01 65.84 ± 6.71 R0 + 5.0% fulvic acid 67.91 ± 3.09 63.98 ± 1.33 R0 + 7.5% fulvic acid 67.39 ± 3.61 66.97 ± 3.67 R0 + 10% fulvic acid 66.78 ± 2.50 64.78 ± 3.67 Average 66.96 ± 1.04 65.28 ± 2.41

Table 4. Effect of fulvic acid on in vitro dry matter digestibilty (IVDMD)

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Table 5. Effect of fulvic acid on in vitro organic matter digestibilty (IVOMD)

Level of Fulvic Acid IVOMD (%) Ration A Ration B Control Ration (R0) 64.84 ± 3.00 63.35 ± 5.09 R0 + 2.5% fulvic acid 65.55 ± 2.39 64.84 ± 6.45 R0 + 5.0% fulvic acid 67.04 ± 2.71 63.50 ± 0.78 R0 + 7.5% fulvic acid 66.59 ± 3.63 66.20 ± 2.77 R0 + 10% fulvic acid 66.74 ± 2.19 64.40 ± 1.57 Average 66.15 ± 0.57 64.46 ± 2.39

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Table 6. Effect of fulvic acid on total volatile fatty acids (VFAs)

Level of Fulvic Acid VFAs (mM) Ration A Ration B Control Ration (R0) 95.08 ± 12.01 112.97 ± 7.08 R0 + 2.5% fulvic acid 94.63 ± 12.14 108.76 ± 17.31 R0 + 5.0% fulvic acid 108.84 ± 6.63 98.80 ± 15.47 R0 + 7.5% fulvic acid 100.59 ± 13.69 97.21 ± 7.09 R0 + 10% fulvic acid 108.30 ± 6.61 100.95 ± 6.68 Average 101.49 ± 3.35 103.74 ± 5.21

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Table 7. Effect of fulvic acid on rumen ammonia (NH3)

Level of Fulvic Acid NH3 (mM) Ration A Ration B Control Ration (R0) 3.82 ± 0.60 4.62 ± 0.65 R0 + 2.5% fulvic acid 4.33 ± 0.66 5.14 ± 0.99 R0 + 5.0% fulvic acid 4.38 ± 0.81 4.81 ± 0.76 R0 + 7.5% fulvic acid 4.05 ± 0.93 4.33 ± 1.38 R0 + 10% fulvic acid 4.63 ± 0.49 4.37 ± 0.58 Average 4.24 ± 0.17 4.65 ± 0.32

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Table 8. Effect of fulvic acid on protozoa population

Level of Fulvic Acid Protozoa Population (x 104/ml) Ration A Ration B Control Ration (R0) 1,63 ± 0,54 1,15 ± 0,24 R0 + 2.5% fulvic acid 1,73 ± 0,36 1,25 ± 0,74 R0 + 5.0% fulvic acid 0,83 ± 0,38 1,81 ± 0,72 R0 + 7.5% fulvic acid 1,27 ± 0,61 1,02 ± 0,49 R0 + 10% fulvic acid 1,10 ± 0,81 1,44 ± 1,13 Average 1,31 ± 0,18 1,33 ± 0,33

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• Fulvic acid supplementation in both complete rations

significantly increase rumen pH but still within the normal level.

• However, treatment of fulvic acid supplementation did

not affect on fermentation characteristics (digestibility

• f dry matter and organic matter, total VFA

concentration, NH3 concentration and rumen protozoa population)

CONCLUSION

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