Interferon- release assay for Coxiella burnetii Q fever Caused by - - PowerPoint PPT Presentation

▶

Oct 20, 2023 192 likes •300 views

Interferon- release assay for Coxiella burnetii Q fever Caused by bacteria Coxiella burnetii Human infections after shedding by animals (goats, sheep, cattle, etc.) Found worldwide except New Zealand Main type of diagnostics: serology Main

SLIDE 1

Interferon-ɣ release assay for Coxiella burnetii

SLIDE 2

Caused by bacteria Coxiella burnetii Human infections after shedding by animals (goats, sheep, cattle, etc.) Found worldwide except New Zealand Main type of diagnostics: serology Main treatment: antibiotics (doxycycline) Largest outbreak: The Netherlands in 2007-2010: 100,000 infections by goats Vaccination programs: in Australia

Q fever

SLIDE 3

First diagnostic test based on cellular immune response In vitro blood stimulation with killed off Coxiella bacteria* Interferon-ɣ production used as response measure

What’ s new?

* Schoffelen et al., 2013

SLIDE 4

Q-detect looks at T-cell response

Serology IgG - IgM Humoral immunity

B-cells

SLIDE 5

Q-detect looks at T-cell response

Q-detect Interferon ɣ Serology IgG - IgM Humoral immunity

B-cells

Cellular immunity

T-cells

+

SLIDE 6

Q-detect way of working

Blood withdrawal (8 ml) Stimulation with killed bacteria in biosafety level 2 laboratory

SLIDE 7

Q-detect way of working

24 h incubation @37⁰C Washing and colouring (ELISA) Interferon-ɣ measurement

SLIDE 8

Results based on individual:

Coxiella induced IFN-γ production (cox - neg)
Relative Coxiella Response vs. PHA

Large study* in high prevalence community compares IFA** to Q-detect ( > 5 years after infection ) Q-detect finds 80% more Q fever infected people

Q-detect results

* Q-Herpen II Study, 2014, >1,500 people included, financed by the Dutch Ministery of Health, Welfare and Sports ** Focus Diagnostics

100 200 300 400 500 600 700 800 900 1000 Q fever positive IFA Q-detect

+68%

SLIDE 9

Validated on > 1,600 volunteers Sensitivity: 94% (at prevalence of 10%) Specificity: > 92% CE marked (Dec 2014) Can be adapted for veterinary use Q-detect in combination with IL-2 investigated for QFS

Product information

SLIDE 10

Innatoss Laboratories

Molenstraat 110, 5342 CC Oss, the Netherlands (Pivot Park, Room RE 2428) Contact: Maarten van der Zanden E: maarten.vanderzanden@innatoss.com T: +31 637353787