Integrate Control for Aedes aegypti Population Supression Margareth - PDF document

Integrate Control for Aedes aegypti Population Supression Margareth L. Capurro mcapurro@icb.usp.br Integrate Control for Aedes aegypti Population Supression 1

Sterile Insect Technique (SIT) 1. MALE STERILISATION Irradiation Birth Control Method:  Mass rearing  Sex separation  Sterilization (irradiation)  Packing, transport, release 2. RELEASE  Sterile matings = no offspring 3. POPULATION SUPPRESSION The Wolbachia suppression approach (IIT/SIT) uninfected infected ♂ ♂ Population Suppression uninfected ♀ infected ♀ 2

SIT X IIT/SIT SIT IIT/SIT • Male sterilization • Male incompatibility • 70 Gy • Female sterilization • 28 – 30 Gy Open Field Release of OX513A Aedes aegypti Transgenic line evaluation 3

R epressive of I nsects carrying a D ominant L ethal gene (RIDL) – From OXITEC Biotech (UK) Antidote (Tetra) death X tTA tetO Minimal promoter Thomas et al. 2000 Science 287: 2474-6 4

Aedes aegypti Production (UPAT) Males for releases 1,5 million/week Itaberaba – Field site 5

PLOS Neglected Tropical Diseases | DOI:10.1371/journal.pntd.0003 864 July 2, 2015 Project Phase 2 – Jacobina - Bahia 6

Pupa transportation (LEMI) 180,000 per container C25 BOD 16 o C ON Arriving at LEMI Preparation for release Emergency , Monitoring and Information Lab Project Phase 2 – Jacobina - Bahia 7

Project Phase 2 – Jacobina - Bahia 80-90% 40-60% How to implement Transgenic mosquitoes in Integrate Control Programs? - Egg distribution is easy - Hatch centers – no larvae sorter - no tetracycline needs - after release no offspring 8

Improving transgenic lines Aedes aegypti and Aedes albopictus • Producing GSS (Genetic Sexing Strain) • Producing Sterile male strain (no Larvae) • Use of tetracycline only in colonies X 9

Sterile Conditional Construct (SCC) 60% Homozygous 80% Reduction Improving transgenic lines Aedes aegypti and Aedes albopictus • Producing GSS (Genetic Sexing Strain) • Producing Sterile male strain (no Larvae) ✔ • Use of tetracycline only in colonies ✔ 10

Production for Release SCC SCC 2017 2016 Capacity: 12 million males for release Moscamed Brasil Universidade de São Paulo UPAT LEMI INCT - EM PRONEX/ DECIT 11