Integrate Control for Aedes aegypti Population Supression Margareth - - PDF document

▶
integrate control for aedes aegypti population supression
SMART_READER_LITE
LIVE PREVIEW

Integrate Control for Aedes aegypti Population Supression Margareth - - PDF document

Jul 14, 2023 13 likes •129 views

Integrate Control for Aedes aegypti Population Supression Margareth L. Capurro mcapurro@icb.usp.br Integrate Control for Aedes aegypti Population Supression 1 Sterile Insect Technique (SIT) 1. MALE STERILISATION Irradiation Birth Control

slide-1
SLIDE 1

1

Integrate Control for Aedes aegypti Population Supression

Margareth L. Capurro mcapurro@icb.usp.br

Integrate Control for Aedes aegypti Population Supression

slide-2
SLIDE 2

2

Sterile Insect Technique (SIT)

Birth Control Method:

  • Mass rearing
  • Sex separation
  • Sterilization (irradiation)
  • Packing, transport, release
  • Sterile matings

= no offspring

  • 1. MALE STERILISATION
  • 2. RELEASE

Irradiation

  • 3. POPULATION SUPPRESSION

uninfected infected

♂ ♂

The Wolbachia suppression approach (IIT/SIT)

uninfected

♀

infected

♀

Population Suppression

slide-3
SLIDE 3

3

SIT X IIT/SIT

SIT

  • Male sterilization
  • 70 Gy

IIT/SIT

  • Male incompatibility
  • Female sterilization
  • 28 – 30 Gy

Open Field Release of OX513A Aedes aegypti Transgenic line evaluation

slide-4
SLIDE 4

4

tTA

Minimal promoter

tetO Antidote (Tetra)

death

Thomas et al. 2000 Science 287: 2474-6

Repressive of Insects carrying a Dominant Lethal gene (RIDL) – From OXITEC Biotech (UK)

X

slide-5
SLIDE 5

5

Aedes aegypti Production (UPAT)

Males for releases 1,5 million/week

Itaberaba – Field site

slide-6
SLIDE 6

6

PLOS Neglected Tropical Diseases | DOI:10.1371/journal.pntd.0003 864 July 2, 2015

Project Phase 2 – Jacobina - Bahia

slide-7
SLIDE 7

7

Pupa transportation (LEMI)

C25

BOD 16oC ON Preparation for release Arriving at LEMI Emergency , Monitoring and Information Lab 180,000 per container

Project Phase 2 – Jacobina - Bahia

slide-8
SLIDE 8

8

Project Phase 2 – Jacobina - Bahia

80-90% 40-60%

How to implement Transgenic mosquitoes in Integrate Control Programs?

  • Egg distribution is easy
  • Hatch centers – no larvae sorter
  • no tetracycline needs
  • after release no offspring
slide-9
SLIDE 9

9

Improving transgenic lines Aedes aegypti and Aedes albopictus

  • Producing GSS (Genetic Sexing Strain)
  • Producing Sterile male strain (no Larvae)
  • Use of tetracycline only in colonies

X

slide-10
SLIDE 10

10

60% Homozygous 80% Reduction

Sterile Conditional Construct (SCC)

Improving transgenic lines Aedes aegypti and Aedes albopictus

  • Producing GSS (Genetic Sexing Strain)
  • Producing Sterile male strain (no Larvae) ✔
  • Use of tetracycline only in colonies ✔
slide-11
SLIDE 11

11

2016 2017

Production for Release

Capacity: 12 million males for release

SCC

SCC

Universidade de São Paulo Moscamed Brasil LEMI UPAT

INCT - EM PRONEX/ DECIT