in Nutraceutical Delivery Rahau Shirazi, Stephen Bloor, Kevin - - PowerPoint PPT Presentation

Nano-vehicles in Nutraceutical Delivery

Rahau Shirazi, Stephen Bloor, Kevin Mitchell

2016 Lipids and Nutraceuticals Conference Nelson November 2016

• Global Nutraceuticals Market
• Lipophilic Vitamins – Encapsulated or Exposed?
• Food grade Nano-vehicles
• Encapsulated Vitamins – Preparation to Characterization
• Conclusion and Future direction

Global Nutraceuticals Market

Analysts forecast the global Functional Food and Nutraceuticals market to grow at a CAGR (compound annual growth rate) of 7.39% during the period 2016-2020. The global dietary supplements market expected growth by 2025 - US$252 billion – from US$131 billion by 2016.

Market Reports World October 28th, 2016

$2.5 billion by 2020 – increases with growing awareness of deficiency links to health problems (e.g.

• steoporosis,
• steomalacia,

and rickets).

Vitamin D Market

Goal: Improve stability during processing and storage of fortified products. Physical and chemical factors: light (UV), heat, moisture, exposure to air, acid or alkaline environments. The biological effects of UV light:

• Degradation
• Safety, ex. formation of reactive species (singlet oxygen and

superoxide radical anion) in case of vitamin A  Damage lipids and DNA

Lipophilic Vitamins – Encapsulated or Exposed?

Food grade Nano-vehicles

Cholecalciferol (Vitamin D3) Retinol (Vitamin A1)

• ZSC-Formula
• C-Formula + Vitamin D3
• SSC-Formula
• SS-Formula + Vitamin A1

Key Characteristics: Food-grade protein-based and lipid- based materials, biodegradable, able to encapsulate lipophilic molecules (e.g. Retinol), easy to handle

SSC-Formula Vitamin D3 C-Formula Vitamin D3 ZSC-Formula Vitamin D3 SBS-Formula + Vitamin A1

Encapsulated vitamin in powder form

ZSC-Formula : Vitamin D3

SEM – ZetaSizer Nano

ZSC-Formula: Vitamin D3 pH Zeta Potential Encapsulated vitamins – Preparation to Characterization

500 nm 500 nm

ZSC-Formula: 4°C to 37°C size increases from 180- 200 nm and zeta potential changes -24 to -19 mv with increase in temperature ZSC-Formula + Vitamin D3: 4°C to 37°C size increases from 180-200 nm with 4.5 microm particles (3%) and zeta potential: -13 at 4°C, -20 mv at 25°C, -11 mv at 37°C

500 nm 500 nm

C-Formula : Vitamin D3

SEM – ZetaSizer Nano

Encapsulated vitamins – Preparation to Characterization

500 nm 500 nm

C-Formula : 4°C to 37°C size increases from 160- 860 nm and zeta potential from

• 10 to -13 mv with increase of temperature.

500 nm 500 nm

C-Formula+Vitamin D3 : 4°C to 37°C size slightly increases from 380-530 nm with microparticles showing(3%) at 25°C, and 80 nm particles (6%) at 37°C and zeta potential is -25 mv at lower temperatures and -23 mv at 37°C.

SSC : Vitamin D3

SEM

500 nm 500 nm 500 nm 500 nm

Preparation: Ethanol-water, Sonication, Shear mixer, Freeze Dried Encapsulated vitamins – Preparation to Characterization Encapsulated vitamin D3 in SSC: 4°C to 37°C size 200-300 nm. Zeta potential: -17 mv at lower temperatures and -22 mv at 37°C.

SS : Vitamin A

SEM

500 nm 500 nm

Preparation: Ethanol-water, Ultra-sonication, Freeze Dried Encapsulated vitamins – Preparation to Characterization Plant Sterols:

European Food Safety Authority (EFSA) authorised claim that allows food manufacturers to state that “plant sterols contribute to the maintenance

• f

normal blood cholesterol levels” with a “daily intake of at least 0.8g of plant sterols”. Ref: Lecithin and Plant Sterols Nutrigold Update Service

Encapsulated

Infant Formula

0.95 1.00 1.05 1.10 1.15 1.20 0.0 2.0 3.0

D3 μg/mL

Infant Formula UV Exposure Vitamin D3 Measurements

0.00 1.00 2.00 3.00 4.00 0.0 0.8 2.0 2.5 18.0 0.00 0.20 0.40 0.60 0.80 1.00 0.0 0.8 2.0 2.5 3.0 18.0

D3 μg/mL

Infant Formula + UV Exposure Vitamin A1 Measurements Infant Formula + UV Exposure Vitamin D3 Measurements

A1 μg/mL

Infant Formula Label Vitamin A 65 μg/100 mL, Vitamin D3 0.95 μg/100 mL

Added: SS Formula + Vitamin A1

Encapsulated vitamins – Preparation to Characterization

Stability of Fortified Milk: Sun Exposure - LCMS Measurements

0.00 1.00 2.00 3.00 4.00 5.00 6.00 0.0 4.5

D3 μg/mL

Conditions: Mixing & Cooling in Quartz Chamber in a sunny day ICED water

QUARTS SHEET

Encapsulated vitamins – Preparation to Characterization Fortified Milk contains vitamin D3

Vitamin D3 in Fortified Milk, UV - LCMS

500 1000 1500 2000 2500

No UV Olive-D3 UV+ Olive-D3 UV D3 μg/mL

Olive-based: Vitamin D3 In Fortified Milk

50 100 150 200 250 300 350 400

No UV Zein SC D3 UV+ Zein SC D3 UV D3 μg/mL

ZSC-Formula + D3 UV+ ZSC-Formula + D3 UV No UV

ZSC-Formula : Vitamin D3 In Fortified Milk

0.05 0.1 0.15 0.2 0.25

No UV Fortified Milk UV+ Fortified Milk UV

D3 μg/mL

Fortified Milk UV+ UV Conditions: varied Volumes no mixing – cooling in UV chamber

(18 hours)

Encapsulated vitamins – Preparation to Characterization

No UV Olive Based Formula D3 UV+ Olive-Based Formula D3 UV

(18 hours) (18 hours)

0.00 5.00 10.00 15.00 20.00 25.00 30.00

0.0 3.0 4.0 4.5 22.0

SSC:D3 in Fortified Milk - UV Exposure Sample Condition: Mixing & Cooling in UV Chamber

SSC : Vitamin D3 in Fortified Milk

D3 μg/mL

UV

Encapsulated vitamins – Preparation to Characterization

1000 2000 3000 4000 0.7 1.5 2 3.5 5.5 18 24 46

Time (hours) Fortified Milk + ZSC-Formula Vitamin D3 Fortified Milk + C-Formula Vitamin D3 Fortified Milk + SSC-Formula Vitamin D3

Encapsulated Vitamin D3 in Fortified Milk UV exposure – LCMS

D3 μg/mL

UV

Conditions: No Mixing - Cooling in UV Chamber

Encapsulated vitamins – Preparation to Characterization

Conclusion and Future Direction

• Stability of bioactive molecules such as vitamins

matters at delivery environment, storage, processing conditions.

• Next we will assess effect of heat, air, acid or

alkaline environments current formulas.

• Future: other vitamins and bioactives in current

and other encapsulation materials

The Integrated Bioactive Technologies Group (IBT) Providing high-value solutions for the industrial biotech sector Lipid Chemistry Create high-value nutraceuticals and cosmeceuticals using our lipid expertise Natural Products and Plant Chemistry Analytical Services Pilot Scale Facilities Process Development Supercritical Fluid Extraction Biology and biochemistry expertise and application to industry Fermentation & Bioprocessing Proteins and bioactive peptides Enzyme Technology

Other Presentations by Lipids Team Andrew McKenzie/Dawn Scott Talk: Natural Sources of Plasmalogens 10th on 11:45am Kirill Lagutin Poster: The use of NMR in the characterization of edible fats and oils. Special Thanks to IBT Group Members especially to Rosemary Webby Funded by Strategic Investment Fund – Callaghan Innovation

Thank You