History SensoQuest SensoQuest History Biotron Biotron GmbH was - - PowerPoint PPT Presentation

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History History SensoQuest SensoQuest

• Biotron

Biotron GmbH was GmbH was founded founded in 1985 in 1985 by by J Jö örg Schrader, rg Schrader, now now CEO of SensoQuest CEO of SensoQuest

• The

The main main business business was: was: Production Production of thermocyclers

• f thermocyclers for

for Biometra Biometra

• 1993

1993 Whatman Whatman bought bought Biometra Biometra

• 1997

1997 Whatman Whatman-

• Biometra

Biometra bought bought Biotron Biotron

• 2001

2001 – – 05 05 Development Development of Labcycler

• f Labcycler
• 2005:

2005: Foundation Foundation of

• f SensoQuest GmbH

SensoQuest GmbH

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Conception Conception of the

• f the

Labcycler Labcycler

• Long life

Long life

• High

High quality quality

• Modern and solid

Modern and solid design design

• Easy

Easy handling handling

• Touchscreen

Touchscreen

• High

High speed speed

• Low

Low energy energy consumption consumption

• Low

Low noise noise in the in the laboratory laboratory

• Universal

Universal block block-

• changing

changing

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High High Quality Quality, Long Life, , Long Life, Modern & Solid Design Modern & Solid Design

• Materials: Gold,

Materials: Gold, Silver Silver, , Copper Copper & Aluminium & Aluminium

• Aluminium front

Aluminium front plate plate, gold , gold coated coated silver silver blocks blocks, , copper copper lids lids

• 6

6 seperately seperately controlled controlled peltier peltier elements elements of the

• f the

newest newest generation generation

• Blocks

Blocks with with self self calibration calibration temperature temperature measuring measuring

• Therfore

Therfore a a tube tube thermistor thermistor is is not not necessary necessary for for calibrating calibrating

• Life Time:

Life Time: More More then then 600,000 600,000 cycles cycles are are tested tested

• 20

20 years years life time in a life time in a standard standard laboratory laboratory

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Display & Keyboard Display & Keyboard

• A modern Touchscreen

A modern Touchscreen

• TFT

TFT ¼ ¼ VGA VGA iluminated iluminated colour colour

• 76,800

76,800 pixel pixel, 5.7 , 5.7“ “diagonal diagonal

• Finger

Finger tapping tapping or

• r Touch

Touch pen pen tapping tapping

• Languages

Languages English and German English and German

• Alphanumeric

Alphanumeric function function keys keys

• Easy

Easy to to use use software software with with selfexplaning selfexplaning icons icons

• Context

Context-

• sensitive

sensitive help help function function

• Graphic

Graphic monitoring monitoring of PCR

• f PCR process

process

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Display & Keyboard Display & Keyboard

Start window Thermoblock 96

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Display & Keyboard Display & Keyboard

Start window Triple Block 3 x 21 [0.2 ml tubes]

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Automatic Lid Automatic Lid

• Heated

Heated lid lid

• Fully

Fully programmable programmable with with the the touch touch screen screen

• Controlled

Controlled by by an an electric electric motor motor

• Power 200 Watt

Power 200 Watt

• Fast:

Fast: Heating Heating rate 1 rate 1 °

°C/s

C/s

• Lid

Lid can can be be deactivated deactivated

• Preheating

Preheating can can be be deactivated deactivated

• Pressure

Pressure 30 to 120 Newton (N) 30 to 120 Newton (N)

• E.g

E.g.: 30 .: 30-

• 60 N

60 N for for single single tubes, 60 to 90 N tubes, 60 to 90 N for for stripes stripes, 90 to 120 N , 90 to 120 N for for microtiterplates microtiterplates

• Hotstart

Hotstart-

• procedure

procedure possible possible

• Pause: Manual and

Pause: Manual and programmable programmable

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Automatic Lid Automatic Lid

Lid Unbolt handling key

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Thermoblocks Thermoblocks

• Thermoblock 48, 96, and 384

Thermoblock 48, 96, and 384 Electroformed Electroformed gold gold plated plated silver silver Thermal Thermal conductivity conductivity 429 W/mK 429 W/mK Heating Heating rate 4.2 rate 4.2 °

°C/s,

C/s, cooling cooling rate 3.6 rate 3.6 °

°C/s

C/s

• Control

Control accuracy accuracy ±

± 0.01

0.01 °

°C

C

• 6

6 seperately seperately controlled controlled peltier peltier elements elements of the

• f the

newest newest generation generation

• Temperature

Temperature – – 5 5 °

°C to 99

C to 99 °

°C

C

• Blocks

Blocks with with self self calibration calibration temperature temperature measuring measuring

• Uniformity

Uniformity ± ± 0.25 0.25 °

°C at 55

C at 55 °

°C,

C, ±

± 0.4

0.4 °

°C at 95

C at 95 °

°C

C

• Ramp rate 0.001

Ramp rate 0.001 °

°C/s to 5.0

C/s to 5.0 °

°C/s

C/s

• De(In)crements

De(In)crements: Temperature : Temperature ±

± 9.99

9.99 °

°C

C

Time Time ±

± 99.99 seconds

99.99 seconds

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Thermoblock 48 Thermoblock 48

48 Wells, 8 zone gradient, 0.5 ml tubes

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Thermoblock 96 Thermoblock 96

96 Wells, 12 zone gradient, 0.2 ml tubes or microtiterplates

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Thermoblock 96 Thermoblock 96

Detail picture of the electroformed gold plated silver

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Thermoblock 384 Thermoblock 384

384 Wells, 24 zone gradient, microtiterplates The unique 384 well silver block

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Triple Block 3 x 21 Triple Block 3 x 21

• Material:

Material: Anodised Anodised aluminium aluminium

• Thermal

Thermal conductivity conductivity 209 W/mK 209 W/mK

• Heating

Heating rate 2.5 rate 2.5 °

°C/s,

C/s, cooling cooling rate 2.2 rate 2.2 °

°C/s

C/s

• 3 independent PCR

3 independent PCR runs runs

• 3 x 21 wells

3 x 21 wells for for 0.2 ml tubes 0.2 ml tubes

• Minimum

Minimum volume volume 10 10 µ µl l

• Protection

Protection against against condensation condensation by by 3 passive 3 passive lids lids

• Seperate

Seperate and parallel and parallel monitoring monitoring of all

• f all blocks

blocks

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Triple Block 3 x 21 Triple Block 3 x 21

Without passive lids

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Triple Block 3 x 21 Triple Block 3 x 21

With one passive lid

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Quick Block Changing Quick Block Changing System System

Picture 1

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Quick Block Changing Quick Block Changing System System

Picture 2

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Quick Block Changing Quick Block Changing System System

Picture 3

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Quick Block Changing Quick Block Changing System System

Picture 4

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Quick Block Changing Quick Block Changing System System

Picture 5

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Auto Re Auto Re-

• Start

Start Function Function

• Line

Line Voltage Voltage 85 V to 265 85 V to 265 without without switching switching, 50 to 60 Hz , 50 to 60 Hz Black Out Black Out during during elongation elongation cycle cycle step step 72 72°

°C,

C, automatic automatic R Re e-

• S

St ta ar rt t at the last at the last denaturation denaturation step step 95 95°

°C

C to to inhibite inhibite unspecific unspecific annealing annealing

• Restart at the last

Restart at the last denaturing denaturing cycle cycle inhibite inhibite unspecific unspecific products products

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User User interface interface

• Alphanumeric

Alphanumeric and and function function keys keys in the in the touchscreen touchscreen

• Special

Special characters characters at at special special

• Figures

Figures at at special special and and numeric numeric silicone silicone keys keys

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Programm Administration Programm Administration

• More

More than than 680 680 programmes programmes

• Classification

Classification in in folder folder, , person person, and , and group group, up to 4 , up to 4 levels levels

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Programm Administration Programm Administration

• Copy

Copy function function via via copy copy and and paste paste

• Intersystem

Intersystem copy copy function function between between two two devices devices also via also via copy copy and and paste paste

• Interfaces: 2* RS232

Interfaces: 2* RS232 for for software software-

• updates

updates

• Software

Software updates updates are are free free and and always always compatible compatible with with old

• ld devices

devices

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Programme INFO Programme INFO

• Runtime

Runtime

• Display of the

Display of the remaining remaining runtime runtime of the

• f the active

active process process (PCR) (PCR)

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Standard PCR Standard PCR

1.

• 1. Initial

Initial Denaturating Denaturating Step Step

• 1

1-

• 5 min, 95

5 min, 95-

• 98

98°

°C

C 2.

• 2. Denaturing

Denaturing Step Step

• 0.5

0.5-

• 2 min, 94

2 min, 94-

• 95

95°

°C

C 3.

• 3. Primer Annealing Step

Primer Annealing Step

• 0.5

0.5-

• 2 min, 5

2 min, 5°

°C

C lower lower than than melting melting temperature temperature ( (e.g e.g. 58 . 58°

°C)

C) 4.

• 4. Extension Step

Extension Step

• 1 min

1 min for for each each 1000 bp 1000 bp using using Taq Taq-

• polymerase, 70

polymerase, 70-

• 75

75°

°C

C 5.

• 5. Number

Number of

• f Cycles

Cycles

• 25

25-

• 35 (max. 40)

35 (max. 40) 6.

• 6. Final Extension Step

Final Extension Step

• 5

5-

• 15 min, 72

15 min, 72°

°C

C 7.

• 7. Storage

Storage after after PCR PCR

• Endless

Endless ( (∞

∞), 4

), 4-

• 8

8°

°C

C

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PCR Programm at the PCR Programm at the Screen Screen

• TFT

TFT-

• Picture

Picture: Standard Programme : Standard Programme

• The

The cycle cycle is is marked marked in in blue blue

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Insert Insert a a Step Step

• The

The new new step step is is marked marked in red in red

• Tapping

Tapping on the

• n the numbers

numbers here here 1 1-

• 5

5

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Online Observation Online Observation

• Graphicel

Graphicel online

• nline monitoring

monitoring of 3 different

• f 3 different PCRs

PCRs in the Triple Block in the Triple Block

• Red

Red is is the block the block left left, , yellow yellow the the middle middle block, and block, and green green the right the right one

• ne

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Help Help Function Function

• Also

Also during during the the process process

• Help

Help library library

• The

The same same information information that that is is written written in the in the manual manual

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Reports Reports

• The last 16

The last 16 programme programme runs runs can can be be displayed displayed any any time ! time !

SLIDE 34

Gradient Gradient Function Function

• 40

40 °

°C,

C, ± ± 20 20 ° °C between the blocks from left to right C between the blocks from left to right

• Thermoblock 48: 8 zones,

Thermoblock 48: 8 zones, -

• 96: 12 zones, 384: 24 zones

96: 12 zones, 384: 24 zones

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Gradient Gradient Function Function

• 40

40 °

°C,

C, ± ± 20 20 ° °C between the blocks from left to right C between the blocks from left to right

• Thermoblock 48: 8 zones,

Thermoblock 48: 8 zones, -

• 96: 12 zones, 384: 24 zones

96: 12 zones, 384: 24 zones

• Green: Maximum

Green: Maximum temperatur temperatur, yellow: middle value, red: lowest value , yellow: middle value, red: lowest value

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Fast Fast Control Control

• With

With deactivated deactivated fast fast control control

• The ramping

The ramping is is smoother smoother

• The PCR

The PCR process process is is 15 % 15 % slower slower

• Interesting

Interesting for for PCR PCR processes processes with with slower ramping, e.g. multiplex PCR slower ramping, e.g. multiplex PCR

• With

With activated activated fast fast control control

• Standard

Standard adjustment adjustment

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Thermal Thermal Conductivity Conductivity

• The

The flow flow of thermal

• f thermal energy

energy through through a a substance substance from from a a higher higher-

• to

to a a lower lower-

• temperature

temperature region region. . OR: OR: Thermal

Thermal conductivity conductivity = = heat heat flow flow rate rate ×

× distance / (

distance / (area area ×

×

temperature temperature difference difference) ) Material Material Thermal Thermal Conductivity Conductivity Diamond Diamond 2000 (W/mK) 2000 (W/mK) Silver Silver

SensoQuest Blocks SensoQuest Blocks

429 429 Gold Gold 318 318 Aluminium Aluminium

Most Most Competitors Competitors Blocks Blocks

205 205 Iron Iron 80 80 Titanium Titanium 21 21 Plastics Plastics

e.g e.g. Tubes . Tubes

1 1 Water Water

e.g e.g. . PCR PCR-

• Reaction

Reaction

0.6 0.6

Important Important for for Heating Heating & & Cooling Cooling Rate Rate

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Edit Edit Program Program

Gradient: 10 °C [± 20 °C] Time in(de)crements: 0.50 s [± 99.99 s] Ramping: 1.5 °C/s [0.001 - 5.0 °C/s] Temperature in(de)crements: – 0.30 °C [± 9.99 °C]

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Temperature Temperature & Time & Time In(de)crements In(de)crements

• Temperature Increments: -9.99°C to + 9.99°C (dT [°C]/Cycle)
• Time Increments: -99.99 sec to + 99.99 sec (dt [s]/Cycle)
• For troubleshooting: E.g, nonspecific bands or poor amplification yield

1)

Annealing temperature is too low: raise the temperature in increments

• f 2°C/(No. of cycles)

2)

Denaturing conditions are too low: optimize the temperature in increments of 0.2°C

3)

Extension time too short: raise in increments of 1 min

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• World wide distributor network: Focus Europe and Asia

www.sensoquest.com www.sensoquest.com