generalist neralist Rhabdom bdomys dilect lectus us in n East - - PowerPoint PPT Presentation

Geneti tic diversit ity y of the forest est dwelling ling Myosorex cafer er and the generalist neralist Rhabdom bdomys dilect lectus us in n East stern ern Cape

MATAMBA E*1 LR RICHARDS2; CHERRY MI1 RAMBAU RV1 1University of Stellenbosch, Department of Botany and Zoology, Stellenbosch 2Department of Mammalogy, Durban Natural Science Museum, Durban

Myosorex cafer Rhabdomys dilectus

Aim

 To compare the genetic structures of forest

dwelling taxa M. cafer and forest associated

• R. dilectus

& Hypothesis

 Forest dwelling species, M. cafer will have

significantly higher genetic structure than generalist species R. dilectus

Localities

Forest st types es Amatole mistbelt Transkei mistbelt Eastern mistbelt Pondoland coastal scarp Transkei coastal scarp (Von Maltitz et al., 2003)

• 1. Fort Fordyce
• 2. Isidenge
• 3. Baziya
• 4. Nqadu
• 5. Gomo
• 6. Ngeli
• 7. Mkhambathi
• 8. Nxumeni

1 2 3 4 5 6 8 7

• M. cafer

Tajima's D: -0.44542 P < 0.05 Number of haplotypes, h: 12 Haplotype diversity, Hd: 0.8995 DnaSP v5 (Librado and Rozas 2009)

Ngeli 105 Ngeli 108 Gomo 77 Gomo 76 Gomo 75 Gomo 73 Gomo 72 Ngeli 109 Ngeli 104 Gomo 71 Ngeli 106 Ngeli 107 Baziya 134 Baziya 135 Baziya 138 Mkhambathi 88 Ngeli 103 Isidenge 27 Isidenge 30 Isidenge 31 Isidenge 33 isidenge 32 Isidenge 28 Isidenge 29 Isidenge 48 Isidenge 58 Fort Fordyce 51 Isidenge 26 Crocidura obscurior

91 91 92 92

71 71 78 78 71 71 79 79 64 64 64 64 64 64 63 63

0.020

COI NJ/ML Kimura-2- parameter (Kimura 1980) In Mega 7 (Tamura et al. 2017)

• R. dilectus

Isidenge 59 Ngeli 112 Fort Fordyce 50 Gomo 94 Mkhambathi 92 Nxumeni 167 Isidenge 64 Nxumeni 162

Isidenge 61

Baziya 140 Gomo 82 Baziya 141 Gomo 99 Gomo 93 Baziya 136 Fort Fordyce 97 Baziya 179 Fort Fordyce 49 Isidenge 46 Nxumeni 157 Baziya 137 Nxumeni 169 Nqadu 102 Baziya 177 Ngeli 111 Isidenge 43 Nxumeni 158 Fort Fordyce 95 Isidenge 34 Gomo 81 Ngeli 114 Isidenge 44 Nqadu 101 Baziya 143 Nxumeni 168 Ngeli 113 Nxumeni 160 Isidenge 41

• utgroup 96

90 90 90 90 66 66 70 70 88 88 90 90 51 51 60 60 68 68 70 70 66 66 42 42 41 41 10 10 0.020

Tajima's D: -2.02551 P < 0.05 Number of haplotypes, h: 25 Haplotype diversity, Hd: 0.9551 DnSP v5 (Librado and Rozas 2009) COI NJ/ML Kimura-2- parameter (Kimura 1980) In Mega 7 (Tamura et al. 2017)

Conclusions

 There is genetic structure for

• M. cafer, and not for R.

dilectus

 Genetic data suggest recent

population size expansions

ACKNOWLEDGEMENTS

 Supervisors  Laboratory mates  Field assistance  National Museums

FBIP

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