Food Safety Laboratories of the future Use of drones, smartphones - - PowerPoint PPT Presentation

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Food Safety Laboratories of the future Use of drones, smartphones - - PowerPoint PPT Presentation

Jun 21, 2023 245 likes •556 views

Food Safety Laboratories of the future Use of drones, smartphones and mobile mass spectrometry Arjen Gerssen Wageningen Food Safety Research So imagine.... WFSR Tools for safety and quality control Production Transport Consumers Inspection

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Food Safety Laboratories of the future

Use of drones, smartphones and mobile mass spectrometry Arjen Gerssen Wageningen Food Safety Research

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So imagine....

WFSR

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Circular economy

Inspection and Control Consumers Retail Production Transport Storage Tools for safety and quality control

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Administration On-site sampling Transportation Lab Screening Few % suspects Lab Confirmation <1% non-compliant

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Current changes

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Paradigm shift Administration On-site sampling Transportation Lab Screening Few % suspects Lab Confirmation <1% non-compliant Administration On-site sampling & Pre-screening Transportation Few % suspects Lab Confirmation Non-compliant

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Current changes

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So imagine....

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Tools for safety and quality control

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  • We’re not machine builders [smartphones, camera’s and equipment

(i.e. mass spectrometers)]

  • Collaborate with machine builders (give constraints and α and β

testers)

  • Hyphenate existing and new technologies
  • Apply them in our area of research (Food and Feed Safety)
  • Bring them to the applicants

The role of WFSR

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Tools for safety and quality control

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  • Precision farming
  • Delivering goods / services
  • Remote sensings

Drones

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Drones and remote sensing

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  • Metal Oxide (MOx) sensors
  • Creating a chemical fingerprint and combine with chemometric

modelling (machine learning)

Drones and remote sensing (electrochemical)

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Drones and remote sensing

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  • Classification model

various types of fires

  • Prediction towards

potential food safety problems (PAHs, PCBs and Dioxins)

  • Remote sampling
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  • Biochemical recognition elements
  • Ultra-fast biorecognition
  • Optical detection using smartphone camera
  • Electrochemical detection via portable potentiostat
  • 3D printed microfluidics
  • Robustness and ease of use
  • Software, server and cloud interfacing
  • App development

Smartphones

11 This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 720325

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  • 3D printed manifold
  • Pre-contained reagents
  • No pipetting skills
  • Finger pumps

Smartphones and LOC

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Suska, A et al., Sci Rep 9, 20320 (2019). https://doi.org/10.1038/s41598-019-55111-z

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  • Applicability of assay – Hazelnut allergens in cookies
  • Allergen LFIA featuring carbon NP labelled secondary mAbs
  • Record-breaking assay speed (30s! for test line development)

Smartphones and allergen detection

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Ross, G.M et al., Biosensors 8, 130 (2018). https://doi.org/10.3390/bios8040130

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  • Read-back by

smartphone

  • Quantitative
  • More reliable

Smartphones and allergen detection

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Ross, G.M et al., Biosensors 8, 130 (2018). https://doi.org/10.3390/bios8040130

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Spectral techniques

15 This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 732541

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Spectral techniques (chemometric models)

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Weesepoel Y. et al., Journal of AOAC INTERNATIONAL (2020) https://doi.org/10.1093/jaoacint/qsaa099

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  • Adulteration of Extra Virgin Olive Oil (EVOO)
  • Data fusion

Spectral techniques

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Weesepoel Y. et al., Journal of AOAC INTERNATIONAL (2020) https://doi.org/10.1093/jaoacint/qsaa099

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Spectral techniques (NIR)

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  • On-site Drug testing (cocaine) handheld near‐infrared

spectrometer and machine learning algorithms

Kranenburg R.F. et al., Drug Testing and Analysis 12(10), 1404-1418 (2020) https://doi.org/10.1002/dta.2895

(A) Cocaine HCl (86.6%) sample (13 scanner 5 spectra each) (B) Cocaine HCl (green line) and adulterated cocaine (red line)

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  • Weight of vacuum systems, power and gas

supplies, high voltage etc.: serious engineering challenges!

  • Easy to operate, WiFi, low weight, libraries
  • Preferably MS/MS analyzers

(Trans)portable lab equipment

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Transportable Multiplex assay (biorecognition) Transportable Mass spectrometry Portable Mass spectrometry Screening Confirmation

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(Trans)portable lab equipment - Luminex

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Toxin Antibody Magnetic beads with ‘toxines’

magnet

Reporter

magnet

Measurement

  • On-site application for shellfish

industry to check for the presence of multiple toxins

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(Trans)portable lab equipment

  • Current workflow in a laboratory

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Samples Extraction Chromatography Ionization Mass Spectrum Samples Ionization Mass Spectrum Direct sample Introduction

  • Workflow for on-site analysis
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Transportable lab equipment (MS)

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Jager J. et al., J. Am. Soc. Mass Spectrom. (2020) https://doi.org/10.1021/jasms.0c00307

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Patent pending

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Simplified read-out for non-technician

Portable lab equipment (MS)

  • No external power or gas supply needed
  • Operational within 10 minutes
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Portable lab equipment (MS)

  • Evaluation for on-site fraud detection with spices
  • Adulterated pepper

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Portable lab equipment (MS)

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TIC real pepper TIC adulterated pepper

50 60 70 80 90 100 110 120 130 140 150 50 100 51 53 55 63 65 67 77 79 91 93 102 105 107 121 136

Limonene

50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 50 100 53 55 65 67 69 77 79 81 89 91 95 105 119 133 147 158 161 176 189 205

Aromadendrene

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  • Validation and accreditation
  • Quality assurance
  • Consumer behaviour when using these tools
  • Technical improvements needed
  • portable MS/MS for selectivity / sensitivity
  • View on the classical laboratories should be changed

Challenges

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Conclusion

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2030 High-end laboratories ‘Personalized’ ‘on-site’ ‘citizen science’ Classical laboratories 2016

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So be prepared!

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Your lab

Circular economy

Inspection and Control Consumers Retail Production Transport Storage Tools for safety and quality control

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Acknowledgement Sandra Munniks Yannick Weesepoel Marco Blokland Michel Nielen Arjen.Gerssen@wur.nl

30 This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 720325 This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 732541

  • Project KB-37-002-005 Innovative tech development

chromatography and mass spectrometry

  • Project KB-37-002-018 Fast On-site method

development

Ministry of Agriculture, Nature and Food Quality

Funding

TKI LWV244, with the title: “Point of Need Testing (PoNT) for transparent, sustainable production chains”.