Effect of hydropriming on germination and seedling vigor of - - PowerPoint PPT Presentation

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Effect of hydropriming on germination and seedling vigor of - - PowerPoint PPT Presentation

Jan 13, 2023 216 likes •508 views

Effect of hydropriming on germination and seedling vigor of pigeonpea [ Cajanus cajan (L.) Millsp.] Katie Carver Iowa State University ICRISAT, Patancheru 10 July 2013 Supervisor: Dr. Myer G Mula Scientist, Pigeonpea Seed Systems Outline

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SLIDE 1

Effect of hydropriming on germination and seedling vigor of pigeonpea [Cajanus cajan (L.) Millsp.]

Katie Carver

Iowa State University

ICRISAT, Patancheru 10 July 2013

Supervisor: Dr. Myer G Mula

Scientist, Pigeonpea Seed Systems

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SLIDE 2

Outline

  • Introduction
  • Objectives
  • Methodology
  • Results
  • Conclusion & Recommendation
  • Acknowledgements
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SLIDE 3
  • Hydropriming-meaning,

importance

  • Mixed results in other

species

  • Is it beneficial to

pigeonpea?

  • What duration of

hydropriming gives the best results?

Introduction

Figure 1. Pigeonpea seeds soaking in water, a process called hydropriming

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SLIDE 4

Objectives

  • Determine the effect of hydropriming on

germination and seedling vigor of pigeonpea

  • Determine the effect of soil type
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SLIDE 5

Methodology

  • Conducted under glasshouse conditions ICRISAT,

Patancheru, Andhra Pradesh, India

  • 5 June to 25 June 2013- rainy season 2013
  • 10 genotypes- medium and early duration

– 4 hybrids, 6 cultivars

  • 4 treatments

– No soaking (control), 2 hrs, 4 hrs, 6 hrs

  • 3 replications

– 5 samples per replication

  • 2 soil types

– Alfisol and Vertisol

Figure 2. Pigeonpea seedlings in the glasshouse

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SLIDE 6
  • Daily irrigation
  • Completely Randomized Block Design (CRD)
  • 5 observations

– Germination on day 3 – Days to 60% germination – Plumule length on day 8 – Plumule length on day 20 – Radicle length on day 20

Methodology

Figure 3. Measuring plumule length on day 8

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Table 1. Characteristics of pigeonpea genotypes Particulars Genotype Seed color 100 seed weight (g) Type Duration Hybrids ICPH 2671 Purple 11.9 NDT Medium ICPH 4431 Light brown 10.6 NDT Medium ICPH 4182 Cream 10.4 NDT Medium ICPH 2740 Brown 9.1 NDT Medium Cultivars ICPL 88039 Brown 9.0 NDT Early ICPL 87091 Brown 13.0 NDT Early ICPL 11811 Black 16.1 NDT Medium ICP 7035 Purple 22.0 NDT Medium ICP 14304 Cream 11.9 NDT Medium ICP 13395 Light brown 19.8 NDT Medium

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Table 2. Analysis of variance (ANOVA) for seedling characteristics across treatment, soil type, and genotype Source of Variation DF Plumule Length- Day 8 Plumule Length- Day 20 Radicle Length- Day 20 Germination

  • n Day 3

Days to 60% Germination Effect of Hydropriming Treatment 3 1.992* 6.375 14.407* 22.544** 6.293** Effect of Soil Type 1 10.965** 213.533** 148.712** 30.817** 6.338** Effect of Hydropriming Treatment x Soil Type 3 0.789 5.495 3.864 2.183 0.026 Effect of Genotype 9 39.061** 383.745** 34.666** 14.739** 2.421** Effect of Hydropriming Treatment x Genotype 27 0.596 3.205 2.695 1.671* 0.512* Effect Soil Type x Genotype 9 2.062** 51.581** 12.166** 7.067** 1.588** Effect of Hydropriming Treatment x Soil Type x Genotype 27 1.099** 3.312 4.158 0.853 0.548* Error 160 0.526 4.463 3.756 0.937 0.317

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Effect of hydropriming Results

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SLIDE 10

c b a c

0.5 1 1.5 2 2.5 No soaking (Control) 2 hr soaking 4 hr soaking 6 hr soaking

Number of seedlings Treatment

Number of seedlings germinated

  • n Day 3
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SLIDE 11

a b c a

3.2 3.4 3.6 3.8 4 4.2 4.4 No soaking (Control) 2 hr soaking 4 hr soaking 6 hr soaking

Days Treatment

Days to 60% Germination

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SLIDE 12

b ab a b

5.5 5.6 5.7 5.8 5.9 6.0 6.1 6.2

No soaking 2 hr soaking 4 hr soaking 6 hr soaking Length (cm) Treatment

Plumule Length- Day 8

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SLIDE 13

a ab a b

10.0 10.2 10.4 10.6 10.8 11.0 11.2 11.4 11.6 11.8 12.0

No soaking 2 hr soaking 4 hr soaking 6 hr soaking Length (cm) Treatment

Radicle Length- Day 20

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Effect of soil type Results

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a b

0.5 1 1.5 2

Alfisol Vertisol Number of seedlings Soil Type

Seedlings germinated on day 3

a b

3.6 3.7 3.8 3.9 4 4.1 4.2

Alfisol Vertisol Days Soil Type

Days to 60% germination

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SLIDE 16

b a a a b b

2 4 6 8 10 12 14 16 18

Plumule- Day 8 Plumule- Day 20 Radicle- Day 20 Length (cm) Plant part

Seedling performance across soil types

Alfisol Vertisol

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Effect of genotype Results

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SLIDE 18

0.5 1 1.5 2 2.5 3 3.5

ICP 14304 ICPL 87091 ICPH 4182 ICPL 88039 ICPH 2671 ICPH 4431 ICP 7035 ICP 11811 ICP 13395 ICPH 2740 Number of seedlings Genotype

Seedlings germinated on Day 3

1 2 3 4 5

ICP 14304 ICPL 87091 ICPH 2671 ICPL 88039 ICPH 4431 ICP 7035 ICP 11811 ICPH 4182 ICPH 2740 ICP 13395 Days Genotype

Days to 60% germination

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SLIDE 19

2 4 6 8 10 12 14 16 18 20 22

ICP 11811 ICP 7035 ICPL 87091 ICP 14304 ICP 13395 ICPH 2671 ICPL 88039 ICPH 4431 ICPH 2740 ICPH 4182

Length (cm) Genotype

Seedling performance across genotypes

Plumule- Day 8 Plumule-Day 20 Radicle- Day 20

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Effect of hydropriming and genotype Results

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2.5 3 3.5 4 4.5 5 ICPH 2671 ICPH 4431 ICPH 4182 ICPH 2740 ICPL 88039 ICPL 87091 ICP 11811 ICP 7035 ICP 14304 ICP 13395 Days Genotype

Days to 60% germination

1 2 3 4 5

ICPH 2671 ICPH 4431 ICPH 4182 ICPH 2740 ICPL 88039 ICPL 87091 ICP 11811 ICP 7035 ICP 14304 ICP 13395 Number of seedlings Genotype

Seedlings germinated on Day 3

No soaking 2 hr soaking 4 hr soaking 6 hr soaking

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Effect of soil type and genotype Results

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0.5 1 1.5 2 2.5 3 3.5 ICPH 2671 ICPH 4431 ICPH 4182 ICPH 2740 ICPL 88039 ICPL 87091 ICP 11811 ICP 7035 ICP 14304 ICP 13395

Number of seedlings Genotype

Seedlings germinated on Day 3

2 2.5 3 3.5 4 4.5 5 ICPH 2671 ICPH 4431 ICPH 4182 ICPH 2740 ICPL 88039 ICPL 87091 ICP 11811 ICP 7035 ICP 14304 ICP 13395

Days Genotype

Days to 60% germination

Alfisol Vertisol

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SLIDE 24

7 9 11 13 15 ICPH 2671 ICPH 4431 ICPH 4182 ICPH 2740 ICPL 88039 ICPL 87091 ICP 11811 ICP 7035 ICP 14304 ICP 13395 Length (cm)

Genotype

Radicle Length- Day 20

5 10 15 20 25 ICPH 2671 ICPH 4431 ICPH 4182 ICPH 2740 ICPL 88039 ICPL 87091 ICP 11811 ICP 7035 ICP 14304 ICP 13395 Length (cm)

Plumule Length- Day 20

Alfisol Vertisol

3 5 7 9

ICPH 2671 ICPH 4431 ICPH 4182 ICPH 2740 ICPL 88039 ICPL 87091 ICP 11811 ICP 7035 ICP 14304 ICP 13395 Length (cm)

Plumule Length- Day 8

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Effect of soil type and genotype and hydropriming treatment Results

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2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0

ICPH 2671 ICPH 4431 ICPH 4182 ICPH 2740 ICPL 88039 ICPL 87091 ICP 11811 ICP 7035 ICP 14304 ICP 13395 ICPH 2671 ICPH 4431 ICPH 4182 ICPH 2740 ICPL 88039 ICPL 87091 ICP 11811 ICP 7035 ICP 14304 ICP 13395 Alfisol Vertisol

Length (cm) Soil type and Genotype Interaction of Genotype, Soil type, and Hydropriming treatment for Plumule Length- Day 8

No soaking 2 hr soaking 4 hr soaking 6 hr soaking

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  • Hydropriming allows for faster seedling

emergence

– Utilize available moisture in rice-fallow systems

  • 4 hour treatments showed best performance

– Longer radicles = more drought-tolerant seedlings – Fastest emergence = successful stand establishment

  • Pigeonpea grows better in Alfisol soils
  • ICP 11811, ICP 7035, and ICP 87091 performed

best with regards to plumule and radicle lengths

  • Conduct experiment in rice-fallow system

Conclusion & Recommendations

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SLIDE 28
  • Plant Sciences Institute, Iowa State

University

  • Learning Systems Unit, ICRISAT
  • Biometrics Department, ICRISAT
  • Pigeonpea Department, ICRISAT

Acknowledgements