Designing DNA Nanomolecular Devices DNA is more than just the secret - - PowerPoint PPT Presentation

Designing DNA Nanomolecular Devices

Presenter: Jenny Kashmirian, 20th January 2005

“DNA is more than just the secret

• f life…

Designing DNA Nanomolecular Devices

“DNA is more than just the secret

• f life…

It is also a versatile component for making nanoscopic structures and devices – Seeman NC”

Presenter: Jenny Kashmirian, 20th January 2005

• Why DNA?
• DNA Structures
• DNA Devices
• Walking DNA
• Rolling DNA
• New Improved Walking DNA
• Hurdles to Overcome
• References

Designing DNA Nanomolecular Devices

Why DNA?

• Strands of DNA interact in a

programmable and predictable way.

• DNA can be induced to form

structures different to a double helix.

• DNA is easy to synthesise
• We have the tools

– CUT -Restriction, exo and endo nucleases – PASTE - ligases

DNA Structures

• Macroscopic Matter Fabrication
• Scaffolding

– Xray Chrystralograpy – Nano Electronics – Chemical Assembly Lines DNA Structures are self assembling nanostructures USES:

DNA Devices would be embedded in these structures

DNA Structures

Truncated Octaheadron Cube Borromean Rings

DNA Structures

• Propensity of DNA to form a double

helix.

• Branched DNA
• Sticky Ends

DNA is a good material because:

DNA Devices

1. Open-Closing Devices eg tweezers DNA Devices are self assembling nanodevices

General Classes of Devices:

DNA Devices – General Classes

2. Extension – Contraction 3. Rotation eg screwdriver

DNA Devices – General Classes

4. Walking Devices

Transportation of a nano-object from one location to another

DNA Walker

Restriction Enzyme Hydrolysis

Features:

• 1. Autonomous
• 2. Bidirectional
• 3. Random
• 4. Translational

Rolling DNA

a a b b A A B B B A a b a b A A B B B A

Hydrolisation “a” is the complement and reverse of “A” with weak hybridisation

Features:

• 1. Autonomous
• 2. Unidirectional
• 3. Random
• 4. Translational &

Rotational

New Improved Walking DNA

A B C A B C A B C A B C A B C

T4 Ligase PlfM I endonuclease T4 Ligase BstAP I endonuclease

Hurdles to Overcome

• Construction MUST be in an

aqueous medium.

• Control of Physical Chemistry.

– What we want – What can happen

• Large #’s molecules required to

read outcome.

References

These were the highlight papers of many more…

• Walking & Rolling DNA – “Design of

Autonomous DNA Nanomechanical Devices: Walking & Rolling DNA”, John Reif.

• Improved Walker – “A Unidirectional DNA Walker

That Moves Autonomously along a Track”, P Yin, H Yan, XG Daniell, AJ Turberfield, J Reif.

• Overview Paper – “Nanotechnology and the

Double Helix”, NC Seeman

Questions?

Molecule Man