Designing DNA Nanomolecular Devices DNA is more than just the secret - PowerPoint PPT Presentation

Designing DNA Nanomolecular Devices “DNA is more than just the secret of life… Presenter: Jenny Kashmirian, 20 th January 2005

Designing DNA Nanomolecular Devices “DNA is more than just the secret of life… It is also a versatile component for making nanoscopic structures and devices – Seeman NC” Presenter: Jenny Kashmirian, 20 th January 2005

Designing DNA Nanomolecular Devices • Why DNA? • DNA Structures • DNA Devices • Walking DNA • Rolling DNA • New Improved Walking DNA • Hurdles to Overcome • References

Why DNA? • Strands of DNA interact in a programmable and predictable way. • DNA can be induced to form structures different to a double helix. • DNA is easy to synthesise • We have the tools – CUT -Restriction, exo and endo nucleases – PASTE - ligases

DNA Structures DNA Structures are self assembling nanostructures USES : • Macroscopic Matter Fabrication • Scaffolding – Xray Chrystralograpy DNA Devices would be – Nano Electronics embedded in – Chemical Assembly Lines these structures

DNA Structures Truncated Octaheadron Cube Borromean Rings

DNA Structures DNA is a good material because: • Propensity of DNA to form a double helix. • Branched DNA • Sticky Ends

DNA Devices DNA Devices are self assembling nanodevices General Classes of Devices: 1. Open-Closing Devices eg tweezers

DNA Devices – General Classes 2. Extension – Contraction 3. Rotation eg screwdriver

DNA Devices – General Classes 4. Walking Devices Transportation of a nano-object from one location to another

DNA Walker Features: Restriction Enzyme 1. Autonomous 2. Bidirectional 3. Random Hydrolysis 4. Translational

Rolling DNA a b b Features: a A B A B A B 1. Autonomous Hydrolisation 2. Unidirectional 3. Random b 4. Translational & a a b Rotational A B A B A B “a” is the complement and reverse of “A” with weak hybridisation

New Improved Walking DNA A B C A B C BstAP I T4 Ligase endonuclease A B C A B C PlfM I T4 Ligase endonuclease A B C

Hurdles to Overcome • Construction MUST be in an aqueous medium. • Control of Physical Chemistry. – What we want – What can happen • Large #’s molecules required to read outcome.

References These were the highlight papers of many more… • Walking & Rolling DNA – “Design of Autonomous DNA Nanomechanical Devices: Walking & Rolling DNA”, John Reif. • Improved Walker – “A Unidirectional DNA Walker That Moves Autonomously along a Track”, P Yin, H Yan, XG Daniell, AJ Turberfield, J Reif. • Overview Paper – “Nanotechnology and the Double Helix”, NC Seeman

Questions? Molecule Man