Department of Zoology MSc Seminar and Project Orientation Programme - - PowerPoint PPT Presentation

Department of Zoology MSc Seminar and Project Orientation Programme

PRESENTATION OF CASE STUDY

By

T emitope O. Sogbanmu, PhD

Date: July 17, 2018

Case Study

❑ A case study in the context of seminar

presentation;

❑ a research article (published in a peer- reviewed journal) ❑ explains / expounds on the seminar topic ❑ should be selected based on local (department, Lagos, Nigeria,…) first before regional

• r

foreign/int’l examples.

April 12, 2020 Presentation of Case Study 2

Seminar Example

T

• pic:

V

• latile Organic

Compounds in Indoor Environments

April 12, 2020 Presentation of Case Study 3

Case Study 1

Title: Influence of V

• latile Organic Solvents’

Inhalation

• n

Activity Quotient and Biochemical Indices of Mus musculus Author(s): Akpan et al. 2012 Aim(s) of the study: to evaluate the effects of volatile organic solvents (xylene, thinner , and gasoline) inhalation on biochemical indices and activity in Mus musculus (laboratory mice)

April 12, 2020 Presentation of Case Study 4

Case Study 1, Cont’d

Methods:

❑A modified nose inhalation exposure method

was used.

❑Exposure chambers were saturated with the

test chemicals (xylene, nitrocellulose thinner and gasoline) for 8 h daily following which they were transferred to the vapour- free section of the chamber .

❑Exposure / study duration: 60 days ❑Average

values for activity quotient were evaluated at days 21, 45 and 60

April 12, 2020 Presentation of Case Study 5

Case Study 1, Cont’d

Methods:

❑ Liver samples were obtained from exposed and

control mice at days 21, 45 and 60 for biochemical studies

❑ Activity quotient was determined thus;

AQ = body movement per min Qty of feed consumed

❑ Biochemical indices evaluated were malondialdehyde

(MDA), reduced gluthathione (GSH), superoxide dismutase (SOD) and catalase (CAT).

April 12, 2020 Presentation of Case Study 6

Case Study 1, Cont’d

Results:

April 12, 2020 Presentation of Case Study 7

Figure 1: Activity quotient of control and exposed mice

• ver a period of 60 days

Case Study 1, Cont’d

Results, Cont’d:

April 12, 2020 Presentation of Case Study 8

Biochemical indices in the exposed and control mice;

• significant decrease (p<0.05) in the levels of

antioxidant enzymes such as reduced gluthathione (GSH), superoxide dismutase (SOD) and catalase (CAT) in exposed mice from day 21 to 60 compared to control.

• the level of lipid peroxidation indicated by

malondialdehyde (MDA) was significantly higher (p<0.05) in exposed mice compared to control.

Case Study 1, Cont’d

Conclusion

April 12, 2020 Presentation of Case Study 9

❑ W

• rkers who are exposed to these volatile
• rganic solvents for elongated periods

may be at risk of liver related disorders. ❑ There is a need for sensitisation and awareness programmes on the use of appropriate personal protection equipment (PPE) and reducing exposure through reduced-time shift regimes.

April 12, 2020 Presentation of Case Study 10