Degradation
Degradation Aggregation
Goals For The Summer
Address Plastic Pollution With the Registry: Proof of Principle artwork by Chris Jordan
Address Plastic Pollution With the Registry: Proof of Principle artwork by Chris Jordan
Create Novel Device to Mimic Oceanic Force
Create Novel Device to Mimic Oceanic Force
Pioneer Marine Chassis for the Registry
Pioneer Marine Chassis for the Registry
Question Access to the Registry with First Public Biobrick
Question Access to the Registry with First Public Biobrick
Conan the Bacterium: The Ancient Microscopic Hero.” Chaotic Utopia. 2006. 16 Feb 2007.
Conan the Bacterium: The Ancient Microscopic Hero.” Chaotic Utopia. 2006. 16 Feb 2007.
Growth Rates In Different Salt Concentrations Growth during exponential phase [h ] -1 LB + 0.3M NaCl LB + 0.6M NaCl LB
Our BioBrick Grows Better Comparing Increase in Exponential Growth Rate Between Biobricks of UCL ‘12 and TU Delft ‘10 in Salt Supplemented Media Percentage Increase in Growth Rates Salt Concentration (M) Our biobrick showed improved growth rates for higher salt concentration than the TU Delft ‘10 biobrick
from The Basics of Biology by Great Pacific Media
from The Basics of Biology by Great Pacific Media
Obtaining Nuclease Synthesise the Nuclease (from Streptococcus Aureus) and dsbA Sequence (Which Transports Nuclease to the Periplasm)
Sequence Design Results in the Transport of Nuclease to Periplasm
BioBrick Toxicity Growth Curve Assay OD (600nm) Time (Hours) BBa_K729019 W3110 (Control)
DNAse Agar Assay
Correlation Between Colony and Halo Diameter for Nuclease Expession of BBa_K729019 Halo Diameter (mm) Colony Diameter (mm)
Halo Diameter (m) Against Mass of Colony (kg) Halo Diameter (m) Mass of Colony (kg)
Use of NahR for Indirect Detection
R O T A N I M R E T R H A N B0015 S B R E V I T U T K228004 I T S N O C R E T O M O B0034 R P J23119 P O OP OP O P R h a N Plastic Betts, K., “Why small plastic particles may pose a big problem in the oceans”, Env. Sci. & Tech. 42 (24), 2008
Triggering Mechanism R h a N P O
Triggering Mechanism R h a N P O
Triggering Mechanism R h a N P O
Curli Construct Design
Congo Red Agar Assay
Shear Device Principle Shear = N - rotational speed Shear Force r - radius - distance between the discs x - viscosity of fluid μ Radius from Centre
Denny, M., “Wave forces on intertidal organisms: A case study’”, American Society of Limnology and Oceanography, Inc., 30(6), 1985, 1171-l 187
Melbourne iGEM 2007
Staying In The Zone Kukulka, T., G. Proskurowski, S. Morét-Ferguson, D. W. Meyer, and K. L. Law (2012), The effect of wind mixing on the vertical distribution of buoyant plastic debris, Geophys. Res. Lett., 39, L07601
Staying In The Zone Kukulka, T., G. Proskurowski, S. Morét-Ferguson, D. W. Meyer, and K. L. Law (2012), The effect of wind mixing on the vertical distribution of buoyant plastic debris, Geophys. Res. Lett., 39, L07601
Staying In The Zone Kukulka, T., G. Proskurowski, S. Morét-Ferguson, D. W. Meyer, and K. L. Law (2012), The effect of wind mixing on the vertical distribution of buoyant plastic debris, Geophys. Res. Lett., 39, L07601
Improved Biobrick Shows a Ten-Fold Increase in Fluorescence Fluorescence Fluorescence Time (min) Time (min) GFP T7 RNAP GFP
Our Improved Biobrick Shows a Three-Fold Better Expression of Fluorescence Over Previous Constructs Relative Fluorescence Time
Measurement of Extracellular Lacasse Activity Using a Syringaldazine Assay OD (530nm) Time (min) Control Laccase Positive Activity of Laccase-Positive Cells is 20-Fold Higher Than Control Cells
High Magnification Low Magnification (10 000x) (100x) Water SEM Results W3110 Lacasse
Measurement of Extracelluar Lacasse Activity Using a Syringaldazine Assay Collaboration with Bielefeld (BBa_K863005) OD (530nm) BL21 + Lacasse W3110 + Lacasse W3110 (Untransformed) Time (mins)
Human Practice
Ethics and Legal UN Legal Feedback Online Discussions Feedback from British Science Association Crowdfunding Speed Debate Public BioBrick iGEM Day Open Day Meeting of Young Minds Family Engagement gemFM Modelling Workshop for LMU iGEM Living Architecture UK Meet-Up Characterisation Paris Collaboration Collaboration with Bielefeld Outreach CAS Conference iGEM community
radio video
Ethics and Legal UN Legal Feedback Online Discussions Feedback from British Science Association Crowdfunding Speed Debate Public BioBrick iGEM Day Open Day Meeting of Young Minds Family Engagement gemFM Modelling Workshop for LMU iGEM Living Architecture UK Meet-Up Characterisation Paris Collaboration Collaboration with Bielefeld Outreach CAS Conference iGEM community
New Measurement Device
14 New BioBricks
14 New BioBricks + 1
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