Degradation Degradation Aggregation Goals For The Summer Address - - PowerPoint PPT Presentation

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Degradation Degradation Aggregation Goals For The Summer Address - - PowerPoint PPT Presentation

Apr 07, 2023 7 likes •862 views

Degradation Degradation Aggregation Goals For The Summer Address Plastic Pollution With the Registry: Proof of Principle artwork by Chris Jordan Address Plastic Pollution With the Registry: Proof of Principle artwork by Chris Jordan

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Degradation

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Degradation Aggregation

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Goals For The Summer

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artwork by Chris Jordan

Address Plastic Pollution With the Registry:

Proof of Principle

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artwork by Chris Jordan

Address Plastic Pollution With the Registry:

Proof of Principle

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Create Novel Device to Mimic Oceanic Force

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Create Novel Device to Mimic Oceanic Force

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Pioneer Marine Chassis for the Registry

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Pioneer Marine Chassis for the Registry

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Question Access to the Registry with First Public Biobrick

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Question Access to the Registry with First Public Biobrick

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Conan the Bacterium: The Ancient Microscopic Hero.” Chaotic Utopia. 2006. 16 Feb 2007.

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Conan the Bacterium: The Ancient Microscopic Hero.” Chaotic Utopia. 2006. 16 Feb 2007.

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Growth Rates In Different Salt Concentrations

LB + 0.3M NaCl LB LB + 0.6M NaCl

  • 1

Growth during exponential phase [h ]

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Comparing Increase in Exponential Growth Rate Between Biobricks of UCL ‘12 and TU Delft ‘10

Our biobrick showed improved growth rates for higher salt concentration than the TU Delft ‘10 biobrick

Percentage Increase in Growth Rates

Salt Concentration (M)

Our BioBrick Grows Better in Salt Supplemented Media

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from The Basics of Biology by Great Pacific Media

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from The Basics of Biology by Great Pacific Media

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Synthesise the Nuclease (from Streptococcus Aureus) and dsbA Sequence (Which Transports Nuclease to the Periplasm)

Obtaining Nuclease

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Sequence Design Results in the Transport of Nuclease to Periplasm

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BBa_K729019 W3110 (Control) Time (Hours)

BioBrick Toxicity Growth Curve Assay

OD (600nm)

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DNAse Agar Assay

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Correlation Between Colony and Halo Diameter for Nuclease Expession of BBa_K729019

Colony Diameter (mm) Halo Diameter (mm)

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Halo Diameter (m) Against Mass of Colony (kg)

Mass of Colony (kg) Halo Diameter (m)

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Use of NahR for Indirect Detection

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B0034

B0015 J23119 K228004

R B S N A H R T E R M I N A T O R C O N S T I T U T I V E P R O M O T E R

N a h R

O P O P OP OP

Plastic

Betts, K., “Why small plastic particles may pose a big problem in the oceans”, Env. Sci. & Tech. 42 (24), 2008

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N a h R

O P

Triggering Mechanism

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N a h R

O P

Triggering Mechanism

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N a h R

O P

Triggering Mechanism

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Curli Construct Design

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Congo Red Agar Assay

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Radius from Centre Shear Force

Shear =

N r x μ

  • rotational speed
  • radius
  • distance between the discs
  • viscosity of fluid

Shear Device Principle

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Denny, M., “Wave forces on intertidal organisms: A case study’”, American Society of Limnology and Oceanography, Inc., 30(6), 1985, 1171-l 187

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Melbourne iGEM 2007

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Staying In The Zone

Kukulka, T., G. Proskurowski, S. Morét-Ferguson, D. W. Meyer, and K. L. Law (2012), The effect of wind mixing

  • n the vertical distribution of buoyant plastic debris, Geophys. Res. Lett., 39, L07601
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Staying In The Zone

Kukulka, T., G. Proskurowski, S. Morét-Ferguson, D. W. Meyer, and K. L. Law (2012), The effect of wind mixing

  • n the vertical distribution of buoyant plastic debris, Geophys. Res. Lett., 39, L07601
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Staying In The Zone

Kukulka, T., G. Proskurowski, S. Morét-Ferguson, D. W. Meyer, and K. L. Law (2012), The effect of wind mixing

  • n the vertical distribution of buoyant plastic debris, Geophys. Res. Lett., 39, L07601
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Fluorescence Time (min)

GFP T7 RNAP

Fluorescence Time (min)

GFP

Improved Biobrick Shows a Ten-Fold Increase in Fluorescence

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Time Relative Fluorescence

Our Improved Biobrick Shows a Three-Fold Better Expression of Fluorescence Over Previous Constructs

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Time (min) OD (530nm)

Laccase Positive Control

Measurement of Extracellular Lacasse Activity Using a Syringaldazine Assay

Activity of Laccase-Positive Cells is 20-Fold Higher Than Control Cells

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SEM Results

Water W3110 Lacasse

Low Magnification (100x) High Magnification (10 000x)

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OD (530nm) Time (mins)

BL21 + Lacasse W3110 + Lacasse W3110 (Untransformed)

Measurement of Extracelluar Lacasse Activity Using a Syringaldazine Assay Collaboration with Bielefeld (BBa_K863005)

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Human Practice

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Meeting of Young Minds Modelling Workshop for LMU iGEM Characterisation Collaboration with Bielefeld CAS Conference Paris Collaboration Living Architecture Family Engagement iGEM Day Open Day UK Meet-Up UN Legal Feedback Feedback from British Science Association Online Discussions Crowdfunding Speed Debate Public BioBrick gemFM

Ethics and Legal iGEM community Outreach

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radio video

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Meeting of Young Minds Modelling Workshop for LMU iGEM Characterisation Collaboration with Bielefeld CAS Conference Paris Collaboration Living Architecture Family Engagement iGEM Day Open Day UK Meet-Up UN Legal Feedback Feedback from British Science Association Online Discussions Crowdfunding Speed Debate Public BioBrick gemFM

Ethics and Legal iGEM community Outreach

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New Measurement Device

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14 New BioBricks

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14 New BioBricks + 1

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