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Chengdu Sichuan University Jun Ju, Kaige Zhang, Chuanqiang Yan, Xu Tong, Mengping Jiang, Yi Zhang
Zhang Logo Background n-alkane degradation ? n-alkane degradation - - PowerPoint PPT Presentation
Zhang Logo Background n-alkane degradation ? n-alkane degradation - - PowerPoint PPT Presentation
Chengdu Sichuan University Jun Ju, Kaige Zhang, Chuanqiang Yan, Xu Tong, Mengping Jiang, Yi Zhang Logo Background n-alkane degradation ? n-alkane degradation Degradation Sensing Emulsification Stress tolerance Biofilm
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n-alkane degradation
?
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n-alkane degradation
Degradation Sensing Emulsification Stress tolerance Biofilm formation
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n-alkane degradation
Degradation Sensing Emulsification
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Outline
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Degradation system
2 main genes:
P450: cytochrom, hydroxylate C8-C20alkane or longer
ALKB2: monooxydase, hydroxylate alkaneC8-C16
Facilitated genes:
AlkJ: medium-chain-length-alcohol dehydrogenase
Aldh: ketone dehydrogenase
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Electronic Chain
Non-haem alkB Hemoprotein Cytochrom
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T emplate vector
Freiburg prefix and surfix: adding NgoMIV and AgeI restriction site to form the scar of ACCGGC
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n-alkane degradation
Degradation Sensing Emulsification
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Source idea
P1: Palks1 P2: Palks2 P3: Palkb
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Our design
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n-alkane degradation
Degradation Sensing Emulsification
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Protein structure
- mpA
β-barrel
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Experiment
Amplification Digestion Ligation Transformation & Expression GCMS
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Methods
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Electron-chain
BBa_K826011 BBa_K826000 BBa_K826008 BBa_K826007 BBa_K826009 BBa_K826006
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Emulcification
0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08 control test1 test2
Oprf: absorbance at 660nm UV
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Human practice and outstretch
iGEM society Weekly workshop Public communication
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Acknowledgement
Instructors: Sponsors: