Chengdu Sichuan University Jun Ju, Kaige Zhang, Chuanqiang Yan, Xu Tong, Mengping Jiang, Yi Zhang
Logo Background
n-alkane degradation ?
n-alkane degradation Degradation Sensing Emulsification Stress tolerance Biofilm formation ··········
n-alkane degradation Degradation Sensing Emulsification
Outline
Degradation system 2 main genes: P450: cytochrom, hydroxylate C 8 -C 20 alkane or longer ALKB2: monooxydase, hydroxylate alkaneC8-C16 Facilitated genes: AlkJ: medium-chain-length-alcohol dehydrogenase Aldh: ketone dehydrogenase
Electronic Chain Non-haem alkB Hemoprotein Cytochrom
T emplate vector Freiburg prefix and surfix: adding NgoMIV and AgeI restriction site to form the scar of ACCGGC
n-alkane degradation Degradation Sensing Emulsification
Source idea P1: Palks1 P2: Palks2 P3: Palkb
Our design
n-alkane degradation Degradation Sensing Emulsification
Protein structure β -barrel ompA
Experiment Amplification Digestion Ligation Transformation & Expression GCMS
Methods
Electron-chain BBa_K826011 BBa_K826000 BBa_K826008 BBa_K826007 BBa_K826009 BBa_K826006
Emulcification Oprf: absorbance at 660nm UV 0.08 0.07 0.06 0.05 0.04 0.03 0.02 0.01 0 control test1 test2
Human practice and outstretch iGEM society Weekly workshop Public communication
Acknowledgement Instructors: Sponsors:
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