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Chengdu Sichuan University Jun Ju, Kaige Zhang, Chuanqiang Yan, Xu Tong, Mengping Jiang, Yi Zhang Logo Background n-alkane degradation ? n-alkane degradation Degradation Sensing Emulsification Stress tolerance Biofilm

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  1. Chengdu Sichuan University Jun Ju, Kaige Zhang, Chuanqiang Yan, Xu Tong, Mengping Jiang, Yi Zhang

  2. Logo Background

  3. n-alkane degradation ?

  4. n-alkane degradation  Degradation  Sensing  Emulsification  Stress tolerance  Biofilm formation ··········

  5. n-alkane degradation  Degradation  Sensing  Emulsification

  6. Outline

  7. Degradation system  2 main genes: P450: cytochrom, hydroxylate C 8 -C 20 alkane or longer  ALKB2: monooxydase, hydroxylate alkaneC8-C16   Facilitated genes: AlkJ: medium-chain-length-alcohol dehydrogenase  Aldh: ketone dehydrogenase 

  8. Electronic Chain Non-haem alkB Hemoprotein Cytochrom

  9. T emplate vector Freiburg prefix and surfix: adding NgoMIV and AgeI restriction site to form the scar of ACCGGC

  10. n-alkane degradation  Degradation  Sensing  Emulsification

  11. Source idea P1: Palks1 P2: Palks2 P3: Palkb

  12. Our design

  13. n-alkane degradation  Degradation  Sensing  Emulsification

  14. Protein structure β -barrel ompA

  15. Experiment  Amplification  Digestion  Ligation  Transformation & Expression  GCMS

  16. Methods

  17. Electron-chain BBa_K826011 BBa_K826000 BBa_K826008 BBa_K826007 BBa_K826009 BBa_K826006

  18. Emulcification Oprf: absorbance at 660nm UV 0.08 0.07 0.06 0.05 0.04 0.03 0.02 0.01 0 control test1 test2

  19. Human practice and outstretch  iGEM society  Weekly workshop  Public communication

  20. Acknowledgement Instructors: Sponsors:

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