Zhang Logo Background n-alkane degradation ? n-alkane degradation - PowerPoint PPT Presentation

Jul 25, 2020 •682 likes •901 views

Chengdu Sichuan University Jun Ju, Kaige Zhang, Chuanqiang Yan, Xu Tong, Mengping Jiang, Yi Zhang Logo Background n-alkane degradation ? n-alkane degradation Degradation Sensing Emulsification Stress tolerance Biofilm

Chengdu Sichuan University Jun Ju, Kaige Zhang, Chuanqiang Yan, Xu Tong, Mengping Jiang, Yi Zhang
Logo Background
n-alkane degradation ?
n-alkane degradation  Degradation  Sensing  Emulsification  Stress tolerance  Biofilm formation ··········
n-alkane degradation  Degradation  Sensing  Emulsification
Outline
Degradation system  2 main genes: P450: cytochrom, hydroxylate C 8 -C 20 alkane or longer  ALKB2: monooxydase, hydroxylate alkaneC8-C16   Facilitated genes: AlkJ: medium-chain-length-alcohol dehydrogenase  Aldh: ketone dehydrogenase 
Electronic Chain Non-haem alkB Hemoprotein Cytochrom
T emplate vector Freiburg prefix and surfix: adding NgoMIV and AgeI restriction site to form the scar of ACCGGC
n-alkane degradation  Degradation  Sensing  Emulsification
Source idea P1: Palks1 P2: Palks2 P3: Palkb
Our design
n-alkane degradation  Degradation  Sensing  Emulsification
Protein structure β -barrel ompA
Experiment  Amplification  Digestion  Ligation  Transformation & Expression  GCMS
Methods
Electron-chain BBa_K826011 BBa_K826000 BBa_K826008 BBa_K826007 BBa_K826009 BBa_K826006
Emulcification Oprf: absorbance at 660nm UV 0.08 0.07 0.06 0.05 0.04 0.03 0.02 0.01 0 control test1 test2
Human practice and outstretch  iGEM society  Weekly workshop  Public communication
Acknowledgement Instructors: Sponsors:

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