Conservation genetics of the rare species Delphinium exaltatum (R - PowerPoint PPT Presentation

Conservation genetics of the rare species Delphinium exaltatum (R ANUNCULACEAE ) of the Appalachian mountains and the Ozark highlands of Missouri Kelsey Huisman Dr. David Bogler Northern Michigan University Missouri Botanical Garden

I NTRODUCTION

objective Examine the genetic variation and differentiation within and among populations of D. exaltatum from the Appalachian mountains and the Ozark highlands of Missouri to distinguish the effects of geographic isolation.

M ETHODS

study sites

• Habitat – Dolomite glades with alkaline soils – An average of 65% shade • Height is between 4 – 6 feet, – Primarily found on southwest to • Individual and at times taller northwest facing slopes flowers are • Cauline • Associated plant species radially life cycle symmetrical • Petiolate – >85% Chinkapin oak ( Quercus with five sepals muehlenbergii ) • April-July: Vegetative • Deeply lobed – Hairy angelica ( Angelica venenosa ) • July-September: Flowering • One of the • August-October: Fruit • Encroaching plant species sepals creates a • Mid to late November-March: Dormant – Cedar ( Juniperus virginiana ) spur – Sugar maple ( Acer saccharum ) • Raceme of 8-30 blue to purple flowers

extractions

amplification

1st PCR cycle

gel-stab technique

2nd PCR cycle

sequencing • Yale University – DNA Analysis Facility • Sanger sequencing Missouri, Howell County sample 11 – large band, forward sequence

R ESULTS

Figure 1. Likelihood analysis. Missouri populations are shown in green.

D ISCUSSION

A CKNOWLEDGEMENTS • National Science Foundation • National Park Service – Daniel Drees – Christine Steinwand • Missouri Botanical Garden – Dr. David Bogler – Justin Zweck – Dr. Rosa Ortiz-Gentry – Missouri Botanical Garden Staff • REU Students

Questions?!