Cancer chemoprevention and identification of antiangiogenic - PowerPoint PPT Presentation

Cancer chemoprevention and identification of antiangiogenic properties of olive oil compounds (Sylvie Lamy, Ph.D. / Chaire en prévention et traitement du cancer – Université du Québec à Montréal, Canada) • Beneficial and preventive role of VEGF-A ! the Mediterranean diet in the occurrence of several chronic diseases including cancer. Taxifolin ! P! P! • These effects have partially been Hydroxytyrosol ! P! P! Tyrosol ! explained by the consumption of P! P! Oleuropein ! Oleic acid ! olive products which contain a VEGFR2 ! range of phenolic compounds, ERK RK JNK JNK P3 P38 pathway pathway ! pathway pathway ! pathway pathway ! (natural antioxidants and highly bioavailable). TAK ! MEKK ! RAF ! MEK1 ! MKK4 ! MKK3 ! • Aim of this study was to investigate MEK2 ! MKK7 ! MKK6 ! and compare the effects of dietary JNK ! ERK ! P38 ! compounds from virgin olive oil on endothelial cell angiogenic PROLIFERATION ! MIGRATION ! process. ANGIOGENESIS ! ! ROP9 Track 1 : Prevention and early detection Disclosure of interest : None declared

Inhibition of endothelial capillary-like tube formation VEGF A Control Tyrosol Oleuropein Control (% of stimulated control) Lenght of tube network 100 75 50 25 0 Ctl Ctl Tyr OL HT Tax OA (50 µ M) VEGF (1 µ g/mL) Hydroxytyrosol Taxifolin Oleic acid Inhibition of endothelial cell proliferation and migration B VEGF (25 ng/mL) VEGF (50 ng/mL) (X-fold of stimulated control) (X-fold of stimulated control) Proliferation Migration 1.0 1.0 Cell proliferation Cell migration 0.5 0.5 0.0 0.0 Ctl Tyr OL Tax HT OA (50 µ M) Ctl Tyr OL Tax HT OA (50 µ M) ROP9 Sylvie Lamy Track 1 : Prevention and early detection Disclosure of interest : None declared

Inhibition of VEGF ligand/receptor (VEGF/VEGFR2) signaling C D VEGF (50 ng/mL) VEGF VEGF VEGF Ctl Ctl HT Ctl Ctl Tax Ctl Ctl OA Ctl Ctl HT Tax Tyr OL OA (50 µM) pVEGFR2 pERK (Tyr951) VEGFR2 ERK pVEGFR2 (Tyr1059) pJNK VEGFR2 JNK pVEGFR2 (Tyr1175) VEGFR2 VEGF Ctl Ctl HT Tax OA pVEGFR2 (Tyr1214) pP38 VEGFR2 P38 Our study demonstrated that HT, Tax and OA are the most potent angiogenesis inhibitors and can have chemopreventive role in cancer through the inhibition of VEGFR2 pathway. These findings highlight the importance of the diet in cancer prevention. ROP9 Sylvie Lamy Track 1 : Prevention and early detection Disclosure of interest : None declared