BACTERIAL CAUSES OF FIN BACTERIAL CAUSES OF FIN ROT IN SOME FRESH - - PowerPoint PPT Presentation

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BACTERIAL CAUSES OF FIN BACTERIAL CAUSES OF FIN ROT IN SOME FRESH WATER ROT IN SOME FRESH WATER FISHES. FISHES.

Enany Enany, M. E., El , M. E., El Sayed Sayed, M. E., , M. E., Diab

• Diab. A. S.,

. A. S., Hassan Hassan, S. M., and El , S. M., and El – – Gamal Gamal, R.M.** , R.M.** *Department of Microbiology, Faculty of Vet. Medicine, *Department of Microbiology, Faculty of Vet. Medicine, Suez Canal University. Suez Canal University. ** Central Lab. For aquaculture, ** Central Lab. For aquaculture, Agr

• Agr. Research Center;

. Research Center; Abbessa Abbessa

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170 naturally infected fishes (90 tilapia spp., 50 Clarias lazera and 30 Commen carp) with fin rot revealed clinically progressive erosions, congestion and hemorrhages of the body fins especially the caudal and dorsal fins with edema and sloughing in some cases .

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naturally infected fishes revealed the presence of 468 bacterial isolates related to 8 bacterial genera and species .

• A. hydrophila (198)
• P. fluorescens (102)

Streptococcus sp.(36)

• F. columnaris (36)

Klebsiella sp. (48)

• E. coli (24)

Proteus sp(12) And Shigٍ ٍ ella sp. (12)

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Table (1): Collective data of bacterial isolates from examined naturally infected fish species.

34.4 34.4 468 468 12 12 (2.6) (2.6) 12 12 (2.6) (2.6) 24 24 (5.1) (5.1) 36 36 (7.7) (7.7) 48 48 ( (10.3 10.3) ) 36 36 (7.7) (7.7) 102 102 (21.8) (21.8) 198 (42.3) 198 (42.3) 170 170 Total Total 6.3 6.3 86 86 1 1 (1.16%) (1.16%) 1 1 (1.16%) (1.16%) 8 8 (9.30%) (9.30%) 9 9 (10.47%) (10.47%) 9 9 ( (10.47% 10.47%) ) 6 6 (6.98%) (6.98%) 18 18 (20.93%) (20.93%) 34 34 (39.53%) (39.53%) 30 30 Carp Carp 10.0 10.0 136 136 4 4 (2.94%) (2.94%) 3 3 (2.21%) (2.21%) 6 6 (4.41%) (4.41%) 9 9 (6.62%) (6.62%) 14 14 ( (10.29% 10.29%) ) 11 11 (8.09%) (8.09%) 30 30 (22.06%) (22.06%) 59 59 (43.38%) (43.38%) 50 50 Clarias Clarias 18.1 18.1 246 246 7 7 (2.85%) (2.85%) 8 8 (3.25%) (3.25%) 10 10 (4.06%) (4.06%) 18 18 (7.32%)) (7.32%)) 25 25 ( (10.16% 10.16%) ) 19 19 (7.73%) (7.73%) 54 54 (21.95%) (21.95%) 105 105 (42.68%) (42.68%) 90 90 Tilapia Tilapia Shegella Shegella Proteus Proteus species species

• E. Coli
• E. Coli

Streptoco Streptoco c c species species Klebsiella Klebsiella species species Flecibacte Flecibacte r r species species Pseudomo Pseudomo nas nas species species Aeromon Aeromon as as species species % % Total Total No. No.

• No. of
• No. of

infected infected fishes with fishes with fin rot fin rot Fish Fish species species

• N. B.: % was calculated according to total number of isolates of each species.

Total No. was calculated according to the total number of the samples.

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Table (2): Distribution of Bacterial isolates among various tissues and organs

• f naturally infected fish species with tail and fin rot.
• 50.00

50.00 3 3 16.67 16.67 1 1

• 33.33

33.33 2 2 6 6 Carp Carp

• 18.18

18.18 2 2 45.45 45.45 5 5

• 36.36

36.36 4 4 11 11 Clarias Clarias

• 36.84

36.84 7 7 31.58 31.58 6 6

• 31.58

31.58 6 6 19 19 Tilapis Tilapis F. F. columnaris columnaris

• 16.67

16.67 3 3 11.11 11.11 2 2

• 5.56

5.56 1 1 27.78 27.78 5 5 16.67 16.67 3 3 22.22 22.22 4 4 18 18 Carp Carp

• 6.67

6.67 2 2 3.33 3.33 1 1

• 6.67

6.67 2 2 3.00 3.00 9 9 20.00 20.00 6 6 33.33 33.33 10 10 30 30 Clarias Clarias

• 12.96

12.96 7 7 5.56 5.56 3 3

• 5.56

5.56 3 3 29.63 29.63 16 16 16.67 16.67 9 9 29.63 29.63 16 16 54 54 Tilapis Tilapis Ps Ps. . fluorscence fluorscence s s

• 2.94

2.94 1 1 14.71 14.71 5 5 5.88 5.88 2 2 20.59 20.59 7 7 11.76 11.76 4 4 14.71 14.71 5 5 29.41 29.41 10 10 34 34 Carp Carp 3.39 3.39 2 2 6.77 6.77 4 4 10.17 10.17 6 6 6.77 6.77 4 4 18.64 18.64 11 11 8.46 8.46 5 5 20.39 20.39 12 12 25.42 25.42 15 15 59 59 Clarias Clarias 3.81 3.81 4 4 6.67 6.67 7 7 12.37 12.37 13 13 5.70 5.70 6 6 17.14 17.14 18 18 8.59 8.59 9 9 18.1 18.1 19 19 37.62 37.62 29 29 105 105 Tilapis Tilapis A. A. hydrophila hydrophila % % No. No. % % No. No. % % No No . . % % N N

• .

. % % No. No. % % No No . . % % No. No. % % No. No. isolate isolate s s Species Species Ascitic fluid Ascitic fluid Ovaries Ovaries Muscle Muscle Spleen Spleen Gills Gills Kidney Kidney Liver Liver Fin & tail Fin & tail lesions lesions Total Total Fish Fish Isolates Isolates

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! !

• Fig. (1) Tilapia
• Fig. (1) Tilapia nilotica

nilotica showing progressive erosions showing progressive erosions

• f body fins, especially caudal and ventral fin, focal to
• f body fins, especially caudal and ventral fin, focal to

diffuse necrosis of muscle and detachment of scales. diffuse necrosis of muscle and detachment of scales.

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! !

• Fig. (2) Tilapia
• Fig. (2) Tilapia nilotica

nilotica showing erosions of body showing erosions of body fins, detachment of scales and skin congestion. fins, detachment of scales and skin congestion.

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! ! Fig.(3) Fig.(3) Clarias Clarias lazera lazera showing erosions and showing erosions and congestion of the body fins. congestion of the body fins.

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The postmortem changes of naturally infected fishes were abdominal ascitis, enlargement and congestion of the liver, kidneys, spleen and intestine with distension and congestion

• f the gall bladder.

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! ! Fig.(4) Common carp showing congestion of Fig.(4) Common carp showing congestion of internal organs specially liver as well as inflamed internal organs specially liver as well as inflamed muscle muscle

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! !

• Fig. (5)Common carp showing detachment of
• Fig. (5)Common carp showing detachment of

scales, erosion of caudal fin, congestion of internal scales, erosion of caudal fin, congestion of internal

• rgans & bloody ascitic fluid.
• rgans & bloody ascitic fluid.

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! !

• Fig. (6) Muscles showing extensive necrosis and
• Fig. (6) Muscles showing extensive necrosis and

focal replacement to the necrotic muscles by edema, focal replacement to the necrotic muscles by edema, hemorrhage, and mononuclear leukocytes hemorrhage, and mononuclear leukocytes

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! !

• Fig. (7) Gills showing edema, congestion, and hemorrhage
• Fig. (7) Gills showing edema, congestion, and hemorrhage

in the gill arch, necrosis and desquamation in the gill in the gill arch, necrosis and desquamation in the gill lamellae along with mononuclear lamellae along with mononuclear leukocytic leukocytic infiltration infiltration

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! !

• Fig. (8) Kidney showing wide spread necrosis of
• Fig. (8) Kidney showing wide spread necrosis of

the renal tubules along with interstitial edema, the renal tubules along with interstitial edema, hemorrhage and mononuclear leukocytes hemorrhage and mononuclear leukocytes

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! !

• Fig. (9) Liver showing extensive vacuolar
• Fig. (9) Liver showing extensive vacuolar

degeneration and focal areas of degeneration and focal areas of coagulative coagulative necrosis necrosis

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The pathogencity of isolated strains revealed that A.hydrophila appeared to be highly virulent ( 87-100%) mortality in injected groups followed by P .fluorescens (50%) and F .columnaris (37.5%)

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Table (3):Route of infection and pattern of mortality in armout catfish experimentally inoculated with fish pathogenic isolated bacteria.

• I/M

I/M Cytophaga Cytophaga 8 8 IX IX

• I/P

I/P Sterile broth Sterile broth 8 8 VIII VIII 87.5 87.5 7 7 1 1

• 1

1

• 1

1 1 1 1 1

• 2

2

• I/M

I/M A.

• A. ydrophila

ydrophila & & P.fluorescens P.fluorescens & & F.columnaris F.columnaris 8 8 VII VII 25 25 2 2

• 1

1

• 1

1

• I/P

I/P P.fluorescens P.fluorescens & & F.columnaris F.columnaris 8 8 VI VI 100 100 8 8

• 1

1

• 1

1

• 1

1 2 2 2 2 1 1

• I/M

I/M A.

• A. ydrophila

ydrophila & & F.columnaris F.columnaris 8 8 V V 87.5 87.5 7 7

• 1

1

• 1

1 1 1 2 2 1 1 1 1

• I/P

I/P A.

• A. ydrophila

ydrophila & & P.fluorescens P.fluorescens 8 8 IV IV 37.5 37.5 3 3

• 1

1

• 1

1

• 1

1

• I/M

I/M F.

• F. olumnaris
• lumnaris

8 8 III III 50 50 4 4 1 1

• 1

1 1 1

• 1

1

• I/P

I/P P.fluorescens P.fluorescens 8 8 II II 100 100 8 8

• 1

1

• 1

1

• 1

1

• 2

2 3 3

• I/P

I/P A.

• A. ydrophila

ydrophila 8 8 I I % % No. No. Total Total 15 15 14 14 13 13 12 12 11 11 10 10 9 9 8 8 7 7 6 6 5 5 4 4 3 3 2 2 1 1 Route of Route of inf. inf. Infected organism Infected organism

• No. fish
• No. fish

Fish Fish group group

8 fish inoculated

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Sensitivity test of isolated strains showed that kanamycin and nalidexic acid were the drugs of choice used for control and treatment of fin rot disease.

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Table(4): Antibiotics sensitivity of Bacteria isolated from naturally infected fish

+2 +2 +4 +4

• +1

+1

• +4

+4 +4 +4

• Carp

Carp ( (6 isolates 6 isolates ( ( +1 +1 +4 +4

• +1

+1 +1 +1 +4 +4 +3 +3

• +1

+1

• +1

+1

• Claris

Claris ( (11 isolates 11 isolates ( ( +2 +2 +4 +4

• +1

+1

• +4

+4 +4 +4

• +1

+1

• +1

+1 Tilapia Tilapia ( (19 isolates 19 isolates ( ( columnaris columnaris . . F F +2 +2 +3 +3 +2 +2 +2 +2 +3 +3 +3 +3 +2 +2

• +1

+1 +2 +2 +2 +2 +1 +1 Carp Carp ( (18 isolates 18 isolates ( ( +3 +3 +2 +2 +1 +1 +3 +3 +4 +4 +3 +3 +1 +1 +1 +1 +1 +1 +2 +2 +2 +2 +2 +2 Claris Claris ( (30 isolates 30 isolates ( ( +3 +3 +2 +2 +2 +2 +3 +3 +4 +4 +4 +4 +2 +2

• +2

+2 +2 +2 +2 +2

• Tilapia

Tilapia ( (54 isolates 54 isolates ( ( fluorscences fluorscences . . Ps Ps +1 +1 +3 +3

• +4

+4 +4 +4 +4 +4 +1 +1 +2 +2 +1 +1 +2 +2 +2 +2 Carp Carp ( (34 isolates 34 isolates ( ( +3 +3 +3 +3

• +3

+3 +3 +3 +4 +4 +3 +3 +1 +1 +1 +1

• +2

+2 +1 +1 Claris Claris ( (59 isolates 59 isolates ( ( +2 +2 +3 +3

• +1

+1 +3 +3 _+4 _+4 +4 +4 +1 +1 +3 +3 +3 +3 +2 +2 +2 +2 Tilapia Tilapia ( (105 isolates 105 isolates ( ( A A. . hydrophila hydrophila SXT SXT TE TE G G S S N N NA NA K K E E D D CL CL C C AM AM X X Antibiotics Antibiotics Fish Fish Species Species Isolates Isolates AMX= Amoxicillin E= Erythromycin G= Sulfonamides C= Chloramphincol K= Kanamycin NA= Nalidixic acid CL= Colistin N= Neomycin SXT= Trimethoprim sulfonamides D= Doxycycline S= Streptomycin Te= Tetracycline

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