Carlos Calvo Garrido Agreenskills Meeting, 12-14 October 2015 - - PowerPoint PPT Presentation

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Carlos Calvo Garrido Agreenskills Meeting, 12-14 October 2015 - - PowerPoint PPT Presentation

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Carlos Calvo Garrido Agreenskills Meeting, 12-14 October 2015 SUMMARY Background The subject: Botrytis bunch rot and biological control The project: Bacterial biological control agents against Botrytis bunch rot and their effects on

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Carlos Calvo Garrido

Agreenskills Meeting, 12-14 October 2015

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SUMMARY

 Background  The subject: Botrytis bunch rot and biological control  The project: Bacterial biological control agents against Botrytis bunch rot and their effects on grape berry microbiota  Expected Outputs

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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BACKGROUND

2006 - Major Degree in Biology by the Autonomous University

  • f Madrid (Spain)

Ecology, Evolutive Biology and Biodiversity Research work on organic agriculture.

2010 - Master in Research on Agricultural and Food Production by the University of Lleida (Spain)

Research work on biological control of fruit pathogens using antagonist microorganisms.

2013 - PhD in Food and Agriculture Science and Technology by the University of Lleida (Spain)

Postharvest Pathology group at IRTA-Lleida (Catalan Institute for Research & Technology in Food & Agriculture; Spain) Thesis title: Efficacy of alternative strategies to synthetic fungicides to control of Botrytis bunch rot (Botrytis cinerea) in organic wine grapes Thesis supervisors: Dr. Neus Teixidó and Pr. Inmaculada Viñas).

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

Universitat de Lleida

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BACKGROUND

PhD in Food and Agriculture Science and Technology by the University of Lleida (Spain)

Other research topics:

  • B. cinerea epidemiology
  • Survival of Biological Control Agents (BCAs) under abiotic factors
  • Sour rot of grapes.

Research stays:

  • 2011 - The New Zealand Institute for Plant and Food Research

Ruakura Research Centre, Hamilton, New Zealand - 5 months

  • 2012 – Institut National de la Recherche Agronomique (INRA)

Centre Bordeaux-Aquitaine, Villenave d’Ornon, France – 6 months

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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BACKGROUND

Calvo-Garrido et al. (2014), Pest Management Science 70: 922-930 Calvo-Garrido et al. (2014), Biological Control. 79: 24-35 Calvo-Garrido et al. (2014), Journal of Applied Microbiology. 117: 800-811 Calvo-Garrido et al. (2014), Journal of Applied Microbiology 116: 967-979 Calvo-Garrido et al. (2014), Pest Management Science 70: 595-602 Calvo-Garrido et al. (2013), Plant Pathology 62: 510-519. Calvo-Garrido et al. (2013), Letters in Applied Microbiology. 57: 356-361

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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Botrytis bunch rot and biological control

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Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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Botrytis cinerea

Filamentous fungi Necrotrophic pathogen Diverse substrates Many economically important plant hosts

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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Botrytis cinerea

Grapevine: Botrytis bunch rot (BBR) Quantity losses Quality losses (Ky et al. 2013)

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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BBR Control

Synthetic fungicide application Development of pathogen resistance Negative effects on environment and health Fungicide use reduction and Alternative control strategies Cultural practices Decision Support Systems / Disease Risk Models Natural products Biological control Combinations!

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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Bacterial biological control agents against Botrytis bunch rot and their effects on grape berry microbiota

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Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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.011 Bacterial Biological Control Agents

Bacterial BCAs and Microbiota

INRA Bordeaux-Aquitaine Research Unit: SAVE (Vineyard Agroecology and Plant Health)

INRA Department of Health-Plant-Environment (SPE)

Project title: Biocontrol strategies against Botrytis in vineyards based on management indicators and developing Biological Control Agents Time period: 3 years Funding: Bordeaux Wine Council (CIVB) Partnerships: IFVV + Chambre Agriculture Gironde + INRA

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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.012 Bacterial Biological Control Agents

Bacterial BCAs and Microbiota

Project title: Biocontrol strategies against Botrytis in vineyards based on management indicators and developing Biological Control Agents Time period: 3 years Funding: Bordeaux Wine Council (CIVB) Partnerships: IFVV + Chambre Agriculture Gironde + INRA

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

Objectives: 1) to test field efficacy of bacterial BCAs, developing recommendations for vineyard applications based on a BBR risk model, 2) to develop specific BCA formulations, using natural products to achieve higher efficacy and best BCA survival in the field 3) to examine the effects of BCA applications on berry microbiota.

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.013 Bacterial Biological Control Agents

Bacterial BCAs and Microbiota

 Previous research: Screening of bacteria isolated from grapevine wood and berries against B. cinerea and Neofusicocum parvum Haidar et al. (in press). Biological Control.

Characterisation mode of action + Survival studies + Combination with additives Isolates selection for field applications

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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.014  Field efficacy: Application on key phenological stages Application based on Disease Risk Model Post-véraison infection model

(Ciliberti et al., in press; Phytopathology)

  • 8 B. cinerea isolates
  • 2 cultivars
  • T x RH combinations

Combination with Natural Products Larger scale applications on commercial vineyards (2017) Bacterial Biological Control Agents

Bacterial BCAs and Microbiota

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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.015 Bacterial Biological Control Agents

Bacterial BCAs and Microbiota

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

Comparison of microbial communities in treated or untreated plots by molecular biology methods: Molecular Footprint (SSCP) Metagenomics (Next-generation Sequencing) Study effect on: fermentative yeasts (wine tipicity), potential BCAs, pathogenic microorganisms…

Determination of the effects of bacteria massive applications on grape berry natural microbiota

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.016 Developing alternative strategies against BBR for growers

Other projects

Objectives:

  • Reference on registered products
  • Determine factor for successful application
  • Optimise its use with the help of other indicators
  • Transfer techniques to growers

Bacterial BCAs?

Project title: BIOBOT, Optimisation of Botrytis bunch rot biocontrol, integrated and global disease management Time period: 3 years Funding: ONEMA – Ministry of Agriculture (France) Partnership: IFVV + Chambre Agriculture Gironde + INRA

Vineyards: Organic winegrowers network of Aquitaine

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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Expected Outputs

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Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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Expected Outputs

 Development of new bacterial BCAs (At which stage?)  Transfer of alternative strategies to growers  Scientific publication

  • Bacterial BCAs and Microbiota project results
  • BIOBOT project results
  • Collaboration for publishing other research of the work team (PhD Rana Haidar)

 New personal research skills:

  • Metagenomics
  • Microbial ecology
  • Vineyard expertise and disease indicators
  • Disease risk modelling

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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Thanks for your attention!

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015

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Thanks for your attention!

Carlos Calvo Garrido/ BACTERIAL BCAs AGAINST BOTRYTIS BUNCH ROT Agreenskills Meeting, 12-14 October 2015