SLIDE 1 Catherine J. Paul
Associate Professor Water Resources Engineering Applied Microbiology Lund University Lund, Sweden Catherine.paul@tvrl.lth.se
Livet i ett dricksvattensystem – nya avancerade analysmetoder ger nya insikter
- m vad som händer mellan vattentäkt och
konsument.
Sandy Chan Peter Rådström Alexander Keucken Kristjan Pullerits Kenneth M. Persson Caroline Schleich
SLIDE 2 Adapted from Proctor and Hammes. 2015. Current opinion in biotechnology 33: 87-94.
The microbial ecosystem of drinking water
Tomorrow: Sandy Chan Tomorrow: Kristjan Pullerits
SLIDE 3
Next Generation DNA Sequencing
16S rRNA Many DNA sequences Inventory of bacteria bioinformatics
SLIDE 4
Different source water or treatment plant, different bacteria
+GW
SLIDE 5 One bacteria at a time DNA-based, no growing One analysis can give:
number of cells number of intact cells
Rapid, simple, and potential for
Flow cytometry
Mälaren Bolmen Kranvatten Loka Grön fluorescens Normaliserat antal bakterier
SLIDE 6
Deep underground….
SLIDE 7
This biofilm is constantly interacting with the water
*not to scale
Biofilm Tap water (cells/mL) Lund: 200 000 Malmö: 470 000 Varberg: 750 000 Stockholm: 300 000 Pipe material*
Bacterium from biofilm Bacterium from Water/DWTP
Microbial Ecosystem
SLIDE 8
How do we find out who is in the biofilm?
IDEA: Too many cells in the water = Not possible to see Look at changes in the cells in the water Changes after the treatment plant from release from biofilm
SLIDE 9
*still not to scale
Biofilm Tap water (cells/mL) Varberg: 750 000 Pipe material*
Bacterium from biofilm Bacterium from Water/DWTP
Installation of UF in Varberg
IDEA:
SLIDE 10
Flow cytometry 16SrRNA amplicon
How many cells move into the water? Who is moving?
SLIDE 11
How many bacteria are leaving the biofilm?
From the biofilm Before UF: 0.5% After UF: 58% From the DWTP Before UF: around 750 000 cells/mL After UF: around 2000 cells/mL
SLIDE 12
Water entering the house
After UF, bacteria from the pipe biofilm are the majority (although less) Before UF, bacteria from treatment plant are the majority
SLIDE 13
Vs.
Single Carbon metabolism
Who is leaving the biofilm?
SLIDE 14
What is the story at the distribution system scale?
SLIDE 15
Are these changes due to biofilm?
? And ultimately… What is a good biofilm? And where does it come from?
SLIDE 16 Keynote speakers Frederik Hammes, EAWAG, Switzerland Isabel Douterelo, University of Sheffield, UK Other confirmed speakers Gang Liu, Oasen and TU Delft, Netherlands Jane Fowler, DTU, Denmark Wilhelm Hijnen, Evides, Netherlands Tarja Pitkänen, National Institute for Health and Welfare, Finland
