Automated Ki67 hot-spot detection and analysis Mieke Zwager MD, - - PowerPoint PPT Presentation
Automated Ki67 hot-spot detection and analysis Mieke Zwager MD, PhD student Department of Pathology University of Groningen, University Medical Center Groningen The Netherlands Background Ki67 Nuclear protein Expressed during cell
MD, PhD student Department of Pathology University of Groningen, University Medical Center Groningen The Netherlands
G1 M S G2 G0 Ki67 low Ki67 high
S.C.Y. Leung et al. Analytical validation of a standardised scoring protocol for Ki67 immunohistochemistry on breast cancer excision whole sections: an international multicentre collaboration. Histopathology 2019: 75;225-235.
S.C.Y. Leung et al. Analytical validationof a standardised scoring protocol for Ki67 immunohistochemistry on breast cancer excision whole sections: an international multicentre collaboration. Histopathology 2019: 75;225-235.
Koopman T. et al. Digital image analysis of Ki67 proliferation index in breast cancer using virtual dual staining on whole tissue sections: clinical validation and inter-platform agreement. Breast Cancer Res Treat. 2018; 169:33-42.
102 breast carcinomas Manual hot-spot detection and scoring
Automated hot-spot detection and scoring DIA manual hot-spot Manual hot-spot detection and scoring
identification
invasive tumor discrimination
quantification
creation
identification
quantification within hot spot
VirtualDoubleStaining™
R² = 0,78
10 20 30 40 50 60 70 80 90 10 20 30 40 50 60 70 80 90 100 Manual Scores Observer 2 Manual Scores Observer 1
R² = 0,90
10 20 30 40 50 60 70 80 90 100 10 20 30 40 50 60 70 80 90 100 Image Analysis Manual Scores
5 10 15 20 25 30 35 40 45 Observer 1 Observer 2 DIA
Mean Ki67 hot-spot score
Mean Ki67 hot-spot score