AnatoliaGreece Anatolia College, Thessaloniki, Greece Construct a - - PowerPoint PPT Presentation

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AnatoliaGreece

Anatolia College, Thessaloniki, Greece “Construct a product that recognizes UV radiation and emits Red Fluorescent Protein (RFP) in response” Foteini Kalampalika, Representing The Team

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Team Roster

1. Christos Papachristou (mascot) 2. Danai Kyriaki (advisor) 3. Dimitris Granouzis 4. Dimitris Gkontoras 5. Athina Prinou 6. Ioanna Deni 7. Kyriakos Zagoumidis 8. Galatia Stefanidou 9. Eliani Economou 10. Lysimachos Papoutsis 11. George Foundos 12. Sotiris Karagkounis 13. Foteini Kalampalika (presenter)

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Last Year’s Project

Worked on improving the efficiency of nitrogen fixing soil bacteria Researched, but decided to choose a different topic

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Research Ideas

We had access to: Google Scholar EBSCO (Elton B Stephens Company) Host Published books and articles found in Eleutheriadis Library (@Anatolia College)

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The Chosen Idea

“To construct a bacterium that recognizes UV radiation and emits RFP in response” The Reasons We Chose The Topic Practical Theoretical

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Practical Theoretical Practical Theoretical

Choose a topic that the genes were in the kit Greece has a lot of sun exposure throughout the year Ordering more genes would take too much time During the summer there is a high risk of sunburn The phenomenon of ozone depletion leads to higher amounts

• f UV entering the atmosphere

Due to the Greenhouse Effect the temperature is constantly increasing These issues are becoming more prominent in many more countries

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Carcinomas and Melanomas

Melanomas are usually diagnosed at a late stage

Rates at which melanoma is increasing Rates of melanoma in different countries in Europe

Ultra Violet Radiation Recognizes UV radiation 09 Emits RFP

(BBa-Jo4450) (Bba-I76500I)

THE PLAN

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What We Plan to Achieve Personal use Collective use

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Personal Use

It will be in bracelet form Like “Sunscreen Bands” but with bacterial instead of photochromic dye Or in a cream or liquid form Put on your skin, if the UV radiation is within the threshold turns red

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Collective Use

Be put on solar panels (in the meteorological centers) so the scientists may analyze the response that the bacterium gives, and then can inform the people

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Pre-Lab

Theoretical background  YouTube videos explaining procedures (ex. Restriction enzymes)  Imperial 2009 team-documentary

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The Lab

We used genes BBa-Jo4450, Bba-I76500I and a connector between them We used protocols given by iGem We used the lab facilities provided by the school

What We Did

Focus on:  understanding background theory  learning how to write a good presentation and posters

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Conclusions

Current achievements participated in a new and unique experience accumulated background knowledge on bacteria and lab procedures learned how to carry

• ut independent

research gained poster and presentation experience will organize next year’s team better

Acknowledgements

The Team The Advisor The Team Leader The school

• Ms. Kitwa Ng for all her help and support for the creation of

This experiment

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