AIA , , TH THE PIN INK FORC RCE A GA INST M AL ARIA GAIN ALARIA - - PowerPoint PPT Presentation

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AIA , , TH THE PIN INK FORC RCE A GA INST M AL ARIA GAIN ALARIA - - PowerPoint PPT Presentation

Nov 18, 2023 342 likes •845 views

A SAI AIA , , TH THE PIN INK FORC RCE A GA INST M AL ARIA GAIN ALARIA Watch our animated movie which explains our project : http://2010.igem.org/Team:EPF_Lausanne/Humanpractices_Movie p2 p25 5 / p / p28 p2 p25 5 / p / p28 p2 p25 5 / p

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ASAI

AIA,

, TH

THE PIN INK FORC RCE

AGA

GAIN INST MAL ALARIA ARIA

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Watch our animated movie which explains our project : http://2010.igem.org/Team:EPF_Lausanne/Humanpractices_Movie

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p2 p25 5 / p / p28

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p2 p25 5 / p / p28

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p2 p25 5 / p / p28

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Im Immun unotox

  • toxin

in p2 p25 / p / p28

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Yoshida et al, Molecular and Biochemical Prasitology, 2000

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pelB FLAG Tag linker Light chain HIS Tag Heavy chain Shiva

Antibody fragment

Pore-forming Toxin

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1st goal

Find a way to deliver these proteins to the mosquito’s gut!

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ASAIA

ADDRESS : mos

  • squito's

ito's intestin estinal al tra ract

SPECIAL CHARACTERISTIC : pink!

k!

ADDITIONAL INFO : speci

ecific fic or

  • rigi

gin

RECENTLY FRIEND WITH : E.

E.col

  • li
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Male terminal portion of mitgut Female terminal portion of mitgut Favia et al., PNAS 2007

Asaia is a commensal bacterium of mosquitoes

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Horizontally Vertically

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Our project

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Find a way to deliver these proteins to the mosquito’s gut!

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2nd goal

Establish Asaia as a new chassis for iGEM

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pH5

Best conditions : 30°C, pH5

Asaia growth characterization

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Asaia GFP in Drosophila

Observation of fluorescent Asaia in the Drosophila's gut 3 hours after infection

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Crotti et al., AEM Acetic acid bacteria, new emerging symbionts of insects, 2010

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Extracted from pHM2-GFP Plasmid Compatible with E. coli

Asaia

  • rigin
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Asaia

  • rigin
  • E. coli
  • rigin

Asaia

  • rigin
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 Characterized Asaia growth  Characterized Asaia’s antibiotic resistance spectrum  Tested Asaia infection in Drosophila  Generated tech sheets for working with Asaia: growth, competent cells, transformation, and cloning

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Establish Asaia as a new chassis

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Construct and characterize BioBricks

3rd goal

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Lysate Supernatant

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Construct and characterize BioBricks

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Human practices

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Express p25, p28, and the Immunotoxin in Asaia Test in vivo (Institute Pasteur) Introduce the engineered Asaia strain in the wild

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Acknowledgements

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