2/9/16 BIOLOGY OPEN HOUSE February 07, 2016 Wednesday; Feb. 10, - - PDF document

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2/9/16 BIOLOGY OPEN HOUSE February 07, 2016 Wednesday; Feb. 10, 2016 Sim 2 Ecology and Evolutionary Biology Neurobiology & Behavior Marine Biology Insect Biology Extracellular recording Atrium of

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February ¡ 07, ¡2016 Sim 2 Extracellular ¡recording Crayfish ¡ Nerve ¡Recording Lab ¡Animal ¡Welfare ¡ ¡Question due ¡Friday, ¡ Feb ¡12

CONCENTRATION INFORMATION FAIR FOR UNDERGRADUATES

RESEARCH EXHIBITS • COURSES • CAREERS • CLUBS •

INSECT BIOLOGY NEUROBIOLOGY & BEHAVIOR

! Explore four concentrations in the

Biology Major

! Talk to faculty about research

pportunities

! Meet other student

concentrators

! Learn about courses ! Explore the neural basis of

behavior

! Embrace biodiversity and

complex ecosystems

! Discover Marine Biology

and Shoals Marine Lab

! Learn about summer

research and courses The Cornell community is invited

MARINE BIOLOGY

Background: Left: Visual cortex of human infant from Ramón y Cajal, S. (1909).. Center: The phylogene tree of vertebrates (Meyer, A. and Zardoya 2003). Right: dragonfly wings, richly diverse in morphological characters used in systematics. ipmnet.org/bugwing Working aboard the R/V Sharp, Marine Biology student Erin Eggleston (Hewson Lab) collects and filters water samples for her study assessing responses of communities of marine microbes to anoxic conditions in the Chesapeake. In the Lost Ladybug Project, Dr. John Losey, Entomology, involves citizen scientists to track distribution patterns of these native and invasive

species. The now rare 9-spot (right) is in decline.

BIOLOGY OPEN HOUSE

Wednesday; Feb. 10, 2016

Ecology and Evolutionary Biology • Neurobiology & Behavior
Marine Biology • Insect Biology •

Atrium of Corson/Mudd Hall 4:00-5:30PM

(215 Tower Road across from Trillium)

If you need accommodations to participate in this event, please contact Saundra Anderson (607-254-4389 or slf42) as soon as possible.

ECOLOGY & EVOLUTIONARY BIOLOGY

To understand the neural basis of communication, the Bass Lab investigates the neural circuits underlying vocal-motor patterning and auditory processing in fish. We also want to know whether behavioral and neural mechanisms, important for communication in fishes, are shared with other vocal vertebrates using investigatory tools ranging from neurophysiology and neuroanatomy to transcriptomics.

No C in space constant

Sim 2 Suction Electrode

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V = IR Amplifier Axon Extracellular fluid Membrane AP to amplifier output How do we apply Ohm’s law? Amplifier Axon Extracellular fluid

resting potential

Differential amplifier Common mode rejection for noise reduction To Oscilloscope/Computer

Amplification of current flowing across amplifier input resistance

Ohm’s Law V = IR

AP current flowing across saline Resistance generates AP voltage cha nge AP current flows across membrane resistance, powered by resting potential V

ltage change causes current

“flow” across metal electrodes

Summary

Electrode metals Bad: Al, Ch, Ti- metal oxide layer (low Ce) Ag, Cu toxic in longer term Good : Gold, SS, Pt- little or no oxide layer

Model Systems in Neuroscience

Large and identified neurons Small neural netw

Crayfish abdominal morphology Introduction to lab exercise for this week

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Key references for this week’s lab: Kennedy and Takeda, 1964b Wine et al 1974 Larimer and Moore 2003 Atwood, 2008

Methylene blue stain of abdominal nervous system

Motor Networks in VNC (Like spinal cord) Alive with reflex activity

Spontaneous activity of nerve 3

0.0 0.4 0.2

100 200 300 400 500 Time (ms) Amplitude (mV) Telson stimulated 0.0 0.4 0.2

100 200 300 400 500 Time (ms) Amplitude (mV) Swimmeret stimulated 0.0 0.4 0.2

100 200 300 400 500 Time (ms) Amplitude (mV) Baseline activity

Single unit analysis: reflex firing of individual axons

different diameters.

Stimulate tail fan Move swimmerets Example student data (last century) Example student data

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Sorting ¡ spikes Goals for lab this week:

How many AP classes? Any patterns of activity in total or individual AP activity? Break down firing activity of different axons Document activity in different sensory stimulation conditions. Activity change due to other reasons?? (time, temperature) Is size of AP related to conduction time? Make sure the computer is making sense. Check AP sizes on Oscilloscope too. Trigger o f f

ne AP and see what else shows up around it. Use the real time recording to verify

different AP shapes.