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• April 11, 2007

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• ftp://ftp.ncbi.nih.gov/genbank/

ftp://genbank.sdsc.edu/pub ftp://bio-mirror.net/biomirror/genbank Release 158 February 2007

87 x 106 Records 157 x 109 Nucleotides

263 Gb (non-WGS) 1115 files .39$/

• full release every two months
• incremental and cumulative updates daily
• available only via ftp
• release notes: gbrel.txt
• Aug-97 Aug-98 Aug-99 Aug-00 Aug-01 Aug-02 Aug-03 Aug-04 Aug-05 Aug-06

20 40 60 80 100 120 140 160

Bases (billions)

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Non-WGS: 69.0 billion bases Non-WGS: 69.0 billion bases WGS: 81.6 billion bases WGS: 81.6 billion bases Release 157 Release 157 Doubling time 12-14 months

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“Organismal”

(Traditional)

PRI (28) Primate ROD (15) Rodent PLN (20) Plant and Fungal BCT (18) Bacterial/Archeal INV (7) Invertebrate VRT (7) Other Vertebrate VRL (4) Viral MAM (2) Mammalian PHG (1) Phage SYN (1) Synthetic ENV (4) Envir. samples UNA (1) Unannotated

“Functional”

(Bulk)

EST (570) Expressed Sequence Tag GSS (197) Genome Survey Sequence HTG (88) High Throughput Genomic PAT (27) Patent STS (9) Sequence Tagged Site CON (1) Contigs, virtual

• Organized by taxonomy (sort of)
• Direct submissions (Sequin/Bankit)
• Accurate (~1 error per 10,000 bp)
• Well characterized
• Organized by sequence type
• Batch submissions (ftp/email)
• Less accurate
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• 80-100,000 unique

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• isolate unique clones
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>IMAGE:275615 3', mRNA sequence NNTCAAGTTTTATGATTTATTTAACTTGTGGAACAAAAATAAACCAGATTAACCACAACCATGCCTTA TTATCAAATGTATAAGANGTAAATATGAATCTTATATGACAAAATGTTTCATTCATTATAACAAATTT AATAATCCTGTCAATNATATTTCTAAATTTTCCCCCAAATTCTAAGCAGAGTATGTAAATTGGAAGTT CTTATGCACGCTTAACTATCTTAACAAGCTTTGAGTGCAAGAGATTGANGAGTTCAAATCTGACCAAG GTTGATGTTGGATAAGAGAATTCTCTGCTCCCCACCTCTANGTTGCCAGCCCTC >IMAGE:275615 5' mRNA sequence GACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTACTCTCTCTTTCTGG TGGAGGTATCCAGCGTACTCCAAAGATTCAGGTTTACTCACGTCATCCAGCAGAGAATGGAAAGTCAA TTCCTGAATTGCTATGTGTCTGGGTTTCATCCATCCGACATTGAAGTTGACTTACTGAAGAATGGAGA GAATTGAAAAAGTGGAGCATTCAGACTTGTCTTTCAGCAAGGACTGGTCTTTCTATCTCTTGTACTAC TGAATTCACCCCCACTGAAAAAGATGAGTATGCCTGCCGTGTTGAACCATGTNGACTTTGTCACAGNC AAGTTNAGTTTAAGTGGGNATCGAGACATGTAAGGCAGGCATCATGGGAGGTTTTGAAGNATGCCGCN TTGGATTGGGATGAATTCCAAATTTCTGGTTTGCTTGNTTTTTTAATATTGGATATGCTTTTG

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• Genomic DNA

Genomic DNA ( (NC NC, , NT NT, , NW NW) ) Model mRNA Model mRNA (XM) (XM)

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Curated mRNA Curated mRNA (NM) (NM)

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Nucleotide PubMed Protein Structure Domains 3D Domains Taxonomy Journals PMC OMIM Books PopSet SNP UniGene UniSTS Genome Gene GEO MeSH CancerChromosomes Homologene PubChem Probe

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