X- -ray ray micobeam micobeam facility for single cells - - PowerPoint PPT Presentation

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X- -ray ray micobeam micobeam facility for single cells irradiations facility for single cells irradiations X S.Bozek 1,2 , J.Bielecki 1 , A.Wiechec 1 , E.Lipiec 1 , J.Lekki 1 , Z.Stachura 1 and W.M.Kwiatek 1 1. Institute of Nuclear Physics,

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X X-

ray micobeam micobeam facility for single cells irradiations facility for single cells irradiations

S.Bozek1,2, J.Bielecki1, A.Wiechec1, E.Lipiec1, J.Lekki1, Z.Stachura1 and W.M.Kwiatek1

1. Institute of Nuclear Physics, Polish Academy of Sciences
2. Jagiellonian University, Medical College
COST MP0601 Dublin

COST MP0601 Dublin Meeting Meeting, 31 , 31 May May 2011 2011

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Outline Outline

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Motivation Motivation %&$! &radiation &cellular level &microbeams

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SLIDE 4

Facility overview Facility overview

Opentype xray source .! α "/ * 010/ .0 2* 0° )! $! 2µ!

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X X ray focusing ray focusing .&)!& ! $

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1000 2000 3000 4000 5000 0,02 2,26 4,50 6,74 8,98 11,22 13,46 15,69 17,93 1000 2000 3000

1 101 201 301 401

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Scintillator material

4 um layer of P43 phosphor
2 um aluminum coating

Focusing process is realised with x-ray sensitive CCD camera

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Microscope alignment in the beam longitudinal direction Microscope alignment in the beam longitudinal direction

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C E L L S C E L L S

' 35 mm !Petri

dishes & 0!! && $&

)! .&)!&& 1.5 'm thick Mylar foil *$$) 105 cells 4 'l medium & $& 16–18 hours

before &4$!

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Positioning calibration Positioning calibration

9,7 9,694 9,688 9,682 9,676 9,67 9,664 9,658 46 63 83 103 121 141 162 181 66 83 103 122 141 160 181 199 84 102 121 142 160 179 200 219 104 121 141 161 179 199 220 238 123 141 161 180 198 218 238 257 142 160 180 199 218 237 258 277 Positioner coordinates [mm]

Position of maxima in the pixels readout

* resolution pattern ) $ ! & micrometer per

pixel calibration ratio &

&!$$ ! #&&) !4! 19,3 ± 0.2 px .&) 6 um ⇒ 0.311 um/px ) ⇒ 60 nm $

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Acknowledgements Acknowledgements

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THANK YOU FOR YOUR ATTENTION THANK YOU FOR YOUR ATTENTION

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Dose calculation Dose calculation first approach with the first approach with the NIST XCOM NIST XCOM database database For 4.5 keV mass attenuation coefficient for water is 58.34 cm2/g Linear attenuation coefficient is 58.34 cm2/g 1g/cm3 = 58.34 / cm

µ µ µ µ = 58.34 1044 / um

Cell thickness x = 10 um For parallel monochromatic beam I = I0 e4 µ

µ µ µ x = 0.94 I0

The spot intensity is 5000 counts / sec 5000 6% = 300 photons/sec deposited in cell. 300 4.5 keV = 1350 keV/s = 2,16 10412 J/s Mass of 10 um in diameter water ball is 4,19 10412 kg The dose is about 500 mGy/s

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Source resolution measurements

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Focal spot size measurements Focal spot size measurements

X=1.0 X=2.0 2 mm

Precise thin copper foil as the edge

Knife edge method

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Calculation of the spot size Calculation of the spot size

4 3 2 1 2 1 P P P x erf P y +               − + =

P1 – beam intensity P2 – coordinates of the peak maximum P3 – width of the peak P4 – bias Obtained curve y is the result of convolution of Gaussian distribution and step function.

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Spot size Spot size